Characterizations of Functions of Biological Materials Having Controlling-Ability Against Ice Crystal Growth

Kawahara, Hidehisa

doi:10.5772/54535

Cited by 7 publications

(7 citation statements)

References 95 publications

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“…When the time to pass through this temperature range is short, a fine ice crystal is formed. On the contrary, when the time is long, a large and rough ice crystal is formed [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

Control of cell proliferation by a porous chitosan scaffold with multiple releasing capabilities

Cai

Weihs

et al. 2017

Science and Technology of Advanced Materials

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The aim of this study was to develop a porous chitosan scaffold with long-acting drug release as an artificial dressing to promote skin wound healing. The dressing was fabricated by pre-freezing at different temperatures (−20 and −80 °C) for different periods of time, followed by freeze-drying to form porous chitosan scaffolds with different pore sizes. The chitosan scaffolds were then used to investigate the effect of the controlled release of fibroblast growth factor-basic (bFGF) and transforming growth factor-β1 (TGFβ1) on mouse fibroblast cells (L929) and bovine carotid endothelial cells (BEC). The biocompatibility of the prepared chitosan scaffold was confirmed with WST-1 proliferation and viability assay, which demonstrated that the material is suitable for cell growth. The results of this study show that the pore sizes of the porous scaffolds prepared by freeze-drying can change depending on the pre-freezing temperature and time via the formation of ice crystals. In this study, the scaffolds with the largest pore size were found to be 153 ± 32 μm and scaffolds with the smallest pores to be 34 ± 9 μm. Through cell culture analysis, it was found that the concentration that increased proliferation of L929 cells for bFGF was 0.005 to 0.1 ng/mL, and the concentration for TGFβ1 was 0.005 to 1 ng/mL. The cell culture of the chitosan scaffold and growth factors shows that 3.75 ng of bFGF in scaffolds with pore sizes of 153 ± 32 μm can promote L929 cell proliferation, while 400 pg of TGFβ1 in scaffolds with pore size of 34 ± 9 μm can enhance the proliferation of L929 cells, but also inhibit BEC proliferation. It is proposed that the prepared chitosan scaffolds can form a multi-drug (bFGF and TGFβ1) release dressing that has the ability to control wound healing via regulating the proliferation of different cell types.

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“…When the time to pass through this temperature range is short, a fine ice crystal is formed. On the contrary, when the time is long, a large and rough ice crystal is formed [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

Control of cell proliferation by a porous chitosan scaffold with multiple releasing capabilities

Cai

Weihs

et al. 2017

Science and Technology of Advanced Materials

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“…IRI is the second function of AFPs [ 11 ]. It explains a thermodynamically encouraging procedure where the larger ice grains are created by the smaller ones possessing high internal energy ( Figure 2 ) [ 12 ].…”

Section: Discussionmentioning

confidence: 99%

Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture

et al. 2020

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Antifreeze proteins (AFPs) are specific proteins, glycopeptides, and peptides made by different organisms to allow cells to survive in sub-zero conditions. AFPs function by reducing the water’s freezing point and avoiding ice crystals’ growth in the frozen stage. Their capability in modifying ice growth leads to the stabilization of ice crystals within a given temperature range and the inhibition of ice recrystallization that decreases the drip loss during thawing. This review presents the potential applications of AFPs from different sources and types. AFPs can be found in diverse sources such as fish, yeast, plants, bacteria, and insects. Various sources reveal different α-helices and β-sheets structures. Recently, analysis of AFPs has been conducted through bioinformatics tools to analyze their functions within proper time. AFPs can be used widely in various aspects of application and have significant industrial functions, encompassing the enhancement of foods’ freezing and liquefying properties, protection of frost plants, enhancement of ice cream’s texture, cryosurgery, and cryopreservation of cells and tissues. In conclusion, these applications and physical properties of AFPs can be further explored to meet other industrial players. Designing the peptide-based AFP can also be done to subsequently improve its function.

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“…This process, termed thermal hysteresis (TH), also alters the ice shape formation depending on the binding site of the proteins to the ice [ 61 ]. Apart from that, AFPs function by inhibiting the nucleation and growth of ice crystals that can cause cell damage, a process called ice recrystallization inhibition (IRI) [ 62 ]. These two features are the main characteristics of AFPs.…”

Section: Physiological Adaptationmentioning

confidence: 99%

Cold Adaptation Strategies and the Potential of Psychrophilic Enzymes from the Antarctic Yeast, Glaciozyma antarctica PI12

Yusof

Hashim

Bharudin

2021

JoF

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Psychrophilic organisms possess several adaptive strategies which allow them to sustain life at low temperatures between −20 to 20 °C. Studies on Antarctic psychrophiles are interesting due to the multiple stressors that exist on the permanently cold continent. These organisms produce, among other peculiarities, cold-active enzymes which not only have tremendous biotechnological potential but are valuable models for fundamental research into protein structure and function. Recent innovations in omics technologies such as genomics, transcriptomics, proteomics and metabolomics have contributed a remarkable perspective of the molecular basis underpinning the mechanisms of cold adaptation. This review critically discusses similar and different strategies of cold adaptation in the obligate psychrophilic yeast, Glaciozyma antarctica PI12 at the molecular (genome structure, proteins and enzymes, gene expression) and physiological (antifreeze proteins, membrane fluidity, stress-related proteins) levels. Our extensive studies on G. antarctica have revealed significant insights towards the innate capacity of- and the adaptation strategies employed by this psychrophilic yeast for life in the persistent cold. Furthermore, several cold-active enzymes and proteins with biotechnological potential are also discussed.

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Characterizations of Functions of Biological Materials Having Controlling-Ability Against Ice Crystal Growth

Cited by 7 publications

References 95 publications

Control of cell proliferation by a porous chitosan scaffold with multiple releasing capabilities

Control of cell proliferation by a porous chitosan scaffold with multiple releasing capabilities

Antifreeze Proteins and Their Practical Utilization in Industry, Medicine, and Agriculture

Cold Adaptation Strategies and the Potential of Psychrophilic Enzymes from the Antarctic Yeast, Glaciozyma antarctica PI12

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