Characterizing cytochrome P450 enzymes involved in plant apocarotenoid metabolism by using an engineered yeast system

Alagöz, Yağiz; Mi, Jianing; Balakrishna, Aparna; Almarwaey, Lamyaa; Al‐Babili, Salim

doi:10.1016/bs.mie.2022.03.003

Cited by 5 publications

(4 citation statements)

References 41 publications

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“…GSTs, UGTs and CYPs are also important metabolic enzymes in various tissues, including brain, where they can mediate the synthesis and metabolism of some endogenous and other substances. Moreover, CYPs have been involved in the metabolism of some bioactive secondary metabolites including carotenoids and apocarotenoids 57 , 58 , which is directly connected with the activation of the Retinal metabolism pathway. In this regard, the transcriptomics data indicates the up-regulation of bcmo-1 (FC = 8.5 in RNA-Seq; FC = 6.3 in RT-qPCR) and bcmo-2 (orthologs of the human bco1 and bco2 ), two genes directly involved in the carotenoid metabolism, catalyzing the oxidative cleavage of β-carotene into two retinal molecules, a form of vitamin A.…”

Section: Discussionmentioning

confidence: 99%

In vivo neuroprotective capacity of a Dunaliella salina extract - comprehensive transcriptomics and metabolomics study

Valdés,

Sánchez-Martínez,

Gallego

et al. 2024

npj Sci Food

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In this study, an exhaustive chemical characterization of a Dunaliella salina (DS) microalga extract obtained using supercritical fluids has been performed, and its neuroprotective capacity has been evaluated in vivo using an Alzheimer’s disease (AD) transgenic model of Caenorhabditis elegans (strain CL4176). More than 350 compounds were annotated in the studied DS extract, with triacylglycerols, free fatty acids (FAs), carotenoids, apocarotenoids and glycerol being the most abundant. DS extract significantly protects C. elegans in a dose-dependent manner against Aβ-peptide paralysis toxicity, after 32 h, 53% of treated worms at 50 µg/mL were not paralyzed. This concentration was selected to further evaluate the transcriptomics and metabolomics changes after 26 h by using advanced analytical methodologies. The RNA-Seq data showed an alteration of 150 genes, mainly related to the stress and detoxification responses, and the retinol and lipid metabolism. The comprehensive metabolomics and lipidomics analyses allowed the identification of 793 intracellular metabolites, of which 69 were significantly altered compared to non-treated control animals. Among them, different unsaturated FAs, lysophosphatidylethanolamines, nucleosides, dipeptides and modified amino acids that have been previously reported as beneficial during AD progression, were assigned. These compounds could explain the neuroprotective capacity observed, thus, providing with new evidences of the protection mechanisms of this promising extract.

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Section: Discussionmentioning

confidence: 99%

In vivo neuroprotective capacity of a Dunaliella salina extract - comprehensive transcriptomics and metabolomics study

Valdés,

Sánchez-Martínez,

Gallego

et al. 2024

npj Sci Food

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“…To discover prenyltransferase (PT) converting 2 to peucenin (3), we obtained one candidate gene SdPT (PCC > 0.95, Supplementary Table 8) among the 20 annotated PT genes. SdPT was sub-cloned to pESC-Leu vector and expressed in yeast WAT11 cells 28 (Supplementary Figs. 10-13).…”

Section: Biosynthesis Of the Furochromone Skeletonmentioning

confidence: 99%

“…Since these enzymes belong to the CYP736 family, we screened candidates from the same family in S. divaricata, and chose four potential genes whose expression levels were highly correlated with the furochromones contents (PCC >0.90, Supplementary Table 10). When SdPC was expressed in yeast WAT11 cells 28 9). HPLC and LC/MS analyses indicated the product had the same retention time and mass spectra with 4 (Fig.…”

Section: Biosynthesis Of the Furochromone Skeletonmentioning

confidence: 99%

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Complete biosynthetic pathway of furochromones and its evolutionary mechanism in Apiaceae plants

Ye,

Zou,

et al. 2024

Preprint

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Furochromones are bioactive and specific secondary metabolites of many Apiaceae plants. Their biosynthesis remains largely unexplored. In this work, we dissected the complete biosynthetic pathway of major furochromones in the medicinal plant Saposhnikovia divaricata by characterizing novel prenyltransferase, peucenin cyclase, methyltransferase, hydroxylase, and glycosyltransferases. De novo biosynthesis of prim-O-glucosylcimifugin and 5-O-methylvisamminoside was then realized in tobacco leaves. Through comparative genomic and transcriptomic analyses, we further found that proximal duplication and high expression of a pentaketide chromone synthase gene SdPCS, together with the presence of a lineage-specific peucenin cyclase gene SdPC, led to the predominant accumulation of furochromones in the roots of S. divaricata among surveyed Apiaceae plants. This study paves the way for metabolic engineering production of furochromones, and sheds light into evolutionary mechanism of furochromone biosynthesis among Apiaceae plants.

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Rice Big Grain1 enhances biomass and plant growth-promoting traits in rhizospheric yeast Candida tropicalis

Ekta,

Biswas,

Mukherjee

et al. 2023

Appl Microbiol Biotechnol

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Characterizing cytochrome P450 enzymes involved in plant apocarotenoid metabolism by using an engineered yeast system

Cited by 5 publications

References 41 publications

In vivo neuroprotective capacity of a Dunaliella salina extract - comprehensive transcriptomics and metabolomics study

In vivo neuroprotective capacity of a Dunaliella salina extract - comprehensive transcriptomics and metabolomics study

Complete biosynthetic pathway of furochromones and its evolutionary mechanism in Apiaceae plants

Rice Big Grain1 enhances biomass and plant growth-promoting traits in rhizospheric yeast Candida tropicalis

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