2021
DOI: 10.1021/acs.chemrev.1c00217
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Characterizing Endogenous Protein Complexes with Biological Mass Spectrometry

Abstract: Biological mass spectrometry (MS) encompasses a range of methods for characterizing proteins and other biomolecules. MS is uniquely powerful for the structural analysis of endogenous protein complexes, which are often heterogeneous, poorly abundant, and refractive to characterization by other methods. Here, we focus on how biological MS can contribute to the study of endogenous protein complexes, which we define as complexes expressed in the physiological host and purified intact, as opposed to reconstituted c… Show more

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Cited by 41 publications
(45 citation statements)
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“…These approaches permit also competitive assays and fragment-based drug design in cells. MS-assisted drug binding was also evaluated from cells overexpressing a protein of interest, using native MS after cell lysis. , …”
Section: Integrating In-cell Nmr In the Field Of In-cell Structural B...mentioning
confidence: 99%
See 2 more Smart Citations
“…These approaches permit also competitive assays and fragment-based drug design in cells. MS-assisted drug binding was also evaluated from cells overexpressing a protein of interest, using native MS after cell lysis. , …”
Section: Integrating In-cell Nmr In the Field Of In-cell Structural B...mentioning
confidence: 99%
“…Structural information and drug binding characterization might be extracted from MS protein-protein interactomes obtained via biotinylation proximity assays, ,, even though these methods are biased toward IDPs . MS-derived structural information has also been obtained recently using cross-linking agents (disuccinimidyl-suberate or -sulfoxide) in cells ,, or in purified mitochondria. , The identified cross-linked peptides inform about their intra- and intermolecular proximities in cells. These great achievements have been carried out upon 0.5–3 h long incubation times in PBS at room temperature.…”
Section: Integrating In-cell Nmr In the Field Of In-cell Structural B...mentioning
confidence: 99%
See 1 more Smart Citation
“…The choice of strategy for selective purification of a bait protein is a critical part of AP-MS experiments [102]. The most straightforward way to achieve selective purification of a protein from cells is to use antibodies.…”
Section: Protein-protein Interactionsmentioning
confidence: 99%
“…Data from tandem mass spectrometry (MS/MS) experiments increasingly provide valuable structural information for proteins and protein complexes. An assortment of different techniques can be used to measure various types of structural information. For example, ion mobility (IM) can provide information on size and shape, chemical cross-linking (XL) can provide information on residue distances and contacts, and covalent labeling can provide information on solvent accessibility and flexibility of residues. The resulting structural information can then be used to better understand the roles of the specific proteins in biological processes. These sparse data have also been combined with computational modeling methods , to improve the accuracy of structural predictions. …”
Section: Introductionmentioning
confidence: 99%