Characterizing human pancreatic cancer precursor using quantitative tissue optical spectroscopy

Lee, Seung Yup; Lloyd, William R.; Chandra, Mudit; Wilson, Robert H.; McKenna, Barbara J.; Simeone, Diane M.; Scheiman, James M.; Mycek, Mary Ann

doi:10.1364/boe.4.002828

Cited by 27 publications

(28 citation statements)

References 21 publications

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“…This result is consistent with ex vivo studies [20][21][22][23][24] and is attributed to differences in scattering between tissue types [22]. Indeed, in vivo reflectance measurements on human pancreatic cancer xenografts grown in mice [20] (Fig.…”

Section: Optical Reflectance Differences Between Normal and Adenocarcsupporting

confidence: 87%

“…Representative in vivo reflectance measurements from human pancreatic adenocarcinoma (blue solid: A, B), (A) normal human tissue (green dashed), and (B) human pancreatic cancer tumor xenograft in a non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mouse (red dashed) [20]. In the diagnostically important wavelength range between 455 and 525 nm (shaded), adenocarcinoma tissues have greater relative reflectance than normal tissues, consistent with extensive ex vivo studies [20][21][22][23][24]. Figure 4 summarizes the results of quantitative analyses applied to in vivo human pancreatic tissue reflectance data.…”

Section: Optical Reflectance Differences Between Normal and Adenocarcsupporting

confidence: 60%

“…Measured spectral data were background subtracted and corrected for the spectral instrument response [20][21][22][23][24]. Reflectance measurements were acquired by delivering broadband white light from a CW tungsten halogen lamp (HL 2000FHSA, Ocean Optics) to tissue.…”

Section: Reflectance and Fluorescence Lifetime Spectrometer (Rfls)mentioning

confidence: 99%

“…Further, optical spectroscopy is compatible with clinical EUS-FNA procedures [10]. Optical techniques investigated in pancreatic tissues include optical coherence tomography [11,12], needle-based confocal laser endomicroscopy [13], near-infrared spectroscopy [14,15], nonlinear optical imaging [16], optical field effect analysis from duodenal tissues [17,18], diffuse optical tomography [19], and our own studies employing optical spectroscopy [20][21][22][23][24]. Advantages to our optical spectroscopy technology include addressing the primary medical need: improved and minimally invasive detection of pancreatic cancer in the presence of overall tissue inflammation.…”

Section: Introductionmentioning

confidence: 99%

“…Malignant tissues were distinguished from benign tissues with sensitivities and specificities of 85% and 86%, respectively [21], and with statistical significance [22] in the setting of chronic pancreatitis. In Stage 3, a PTI model was employed to detect a pancreatic cancer precursor [24]. Here, we address Stage 4 by reporting the in vivo feasibility of optical spectroscopy to detect malignant tissues.…”

Section: Introductionmentioning

confidence: 99%

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In vivo optical spectroscopy for improved detection of pancreatic adenocarcinoma: a feasibility study

Lloyd

Wilson

Lee

et al. 2013

Biomed. Opt. Express

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Pancreatic adenocarcinoma has a five-year survival rate of less than 6%. This low survival rate is attributed to the lack of accurate detection methods, which limits diagnosis to late-stage disease. Here, an in vivo pilot study assesses the feasibility of optical spectroscopy to improve clinical detection of pancreatic adenocarcinoma. During surgery on 6 patients, we collected spectrally-resolved reflectance and fluorescence in vivo. Site-matched in vivo and ex vivo data agreed qualitatively and quantitatively. Quantified differences between adenocarcinoma and normal tissues in vivo were consistent with previous results from a large ex vivo data set. Thus, optical spectroscopy is a promising method for the improved diagnosis of pancreatic cancer in vivo. ©2013 Optical Society of America

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Section: Optical Reflectance Differences Between Normal and Adenocarcsupporting

confidence: 87%

Section: Optical Reflectance Differences Between Normal and Adenocarcsupporting

confidence: 60%

Section: Reflectance and Fluorescence Lifetime Spectrometer (Rfls)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

In vivo optical spectroscopy for improved detection of pancreatic adenocarcinoma: a feasibility study

Lloyd

Wilson

Lee

et al. 2013

Biomed. Opt. Express

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Characterization of thermal and optical properties in porcine pancreas tissue

et al. 2022

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Background: Photothermal therapies have shown promise for treating pancreatic ductal adenocarcinoma when they can be applied selectively, but off-target heating can frustrate treatment outcomes. Improved strategies leveraging selective binding and localized heating are possible with precision medical approaches such as functionalized gold nanoparticles, but careful control of optical dosage and thermal generation would be imperative. However, the literature review revealed many groups assume liver properties for pancreas tissue or rely on insufficiently rigorous characterization studies. Objective: The objective of this study was to determine the thermal conductivity and optical properties at 808/1064 nm wavelengths in healthy samples of fresh and frozen porcine pancreas ex vivo. Methods: Thermal conductivity of the porcine pancreas tissue was measured by utilizing a hot plate and two K-type thermocouples. Experimental variables such as tissue sample thickness, hot plate temperature, and heat convection coefficient were estimated through the control experiments utilizing specimens with known thermal conductivity. Optical evaluations assessed light attenuation at the 808 and 1064 nm wavelengths (continuous wave, collimated beam) by measuring the light transmittance and reflectance of different tissue thicknesses. In turn, these measurements were input into an inverse adding-doubling program to estimate the optical absorption and reduced scattering coefficients. Results: Interestingly, pancreas tissue thermal conductivity was demonstrated to have no significant difference (p > 0.5) between samples that were fresh, frozen for 7 days, or frozen for 14 days. Conversely, optical property assessment exhibited a significant difference (p < 0.001) between fresh and frozen tissue samples, with increased absorbance and reflectance within the frozen group. However, the optical attenuation values measured were substantially less than that of the liver or reported in previous pancreas studies, suggesting a wide overestimation of these properties. Conclusions: These thermal and optical properties are critical to the development of novel therapeutic strategies like plasmonic photothermal therapy, but perhaps more importantly, are invaluable towards informing better surgical planning and operative technique among the existing thermal approaches for treating pancreas tissue. K E Y W O R D Sabsorption coefficient, fresh versus frozen porcine pancreas tissue, porcine pancreas optical properties, porcine pancreas thermal conductivity, scattering coefficient

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