Characterizing lysine acetylation of <i>Escherichia coli</i> type <scp>II</scp> citrate synthase

Venkat, Sumana; Chen, Hao; McGuire, Paige; Stahman, Alleigh; Gan, Qinglei; Fan, Chenguang

doi:10.1111/febs.14845

Cited by 30 publications

(39 citation statements)

References 42 publications

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“…Although it has been proposed that bud dormancy release is associated with the cellular metabolism (Beauvieux et al, 2018), there is no evidence showing how the cellular metabolism changes exactly during dormancy release. Previous studies in other species have shown an extensive role of Kac in regulation of cellular metabolism (Venkat et al, 2019; Zhang, Qu, et al, 2017; Zhang, Zhang, et al, 2017; Zhao et al, 2010). For example, in human cells, enoyl‐CoA hydratase‐1 (ECHS1; EC 4.2.1.17) plays a key role in fatty acid metabolism, acetylation of ECHS1 decreases its activity (Zhang, Qu, et al, 2017; Zhang, Zhang, et al, 2017).…”

Section: Discussionmentioning

confidence: 97%

“…Malate dehydrogenase (MDH, EC 1.1.1.37) catalyzes the NAD/NADH‐dependent inter‐conversion of the substrates malate and oxaloacetate in the TCA cycle, and acetylation of MDH activates its activity (Zhao et al, 2010). Citrate synthase (CS, EC 2.3.3.1) catalyzes the first reaction of the TCA cylce, and Kac of CS could decrease the overall CS activity in Escherichia coli (Venkat et al, 2019). In our study, we checked the effect of Kac modifications on PDH and IDH, another two key enzymes involved in pyruvate metabolism and the TCA cycle.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies in other species have shown an extensive role of Kac in regulation of cellular metabolism (Venkat et al, 2019;Zhang, Qu, et al, 2017;Zhao et al, 2010). For example, in human cells, enoyl-CoA hydratase-1 (ECHS1; EC 4.2.1.17) plays a key role in fatty acid metabolism, acetylation of ECHS1 decreases its activity (Zhang, Qu, et al, 2017; (Venkat et al, 2019).…”

Section: Profound Effect Of Kac Modification On Cellular Processes During Tree Dormancy Releasementioning

confidence: 99%

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Poplar acetylome profiling reveals lysine acetylation dynamics in seasonal bud dormancy release

Liao

Zhen-zhu

et al. 2021

Plant Cell & Environment

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For perennials in boreal and temperate ecosystems, bud dormancy is crucial for survival in harsh winter. Dormancy is released by prolonged exposure to low temperatures and is followed by reactive growth in the spring. Lysine acetylation (Kac) is one of the major post-translational modifications (PTMs) that are involved in plant response to environmental signals. However, little information is available on the effects of Kac modification on bud dormancy release. Here, we report the dynamics of lysine acetylome in hybrid poplar (Populus tremula × Populus alba) dormant buds. A total of 7,594 acetyl-sites from 3,281 acetyl-proteins were identified, representing a large dataset of lysine acetylome in plants. Of them, 229 proteins were differentially acetylated during bud dormancy release and were mainly involved in the primary metabolic pathways. Site-directed mutagenesis enzymatic assays showed that Kac strongly modified the activities of two key enzymes of primary metabolism, pyruvate dehydrogenase (PDH) and isocitrate dehydrogenase (IDH). We thus propose that Kac of enzymes could be an important strategy for reconfiguration of metabolic processes during bud dormancy release. In all, our results reveal the importance of Kac in bud dormancy release and provide a new perspective to understand the molecular mechanisms of seasonal growth of trees.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Section: Profound Effect Of Kac Modification On Cellular Processes During Tree Dormancy Releasementioning

confidence: 99%

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Poplar acetylome profiling reveals lysine acetylation dynamics in seasonal bud dormancy release

Liao

Zhen-zhu

et al. 2021

Plant Cell & Environment

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“…CheY is an essential regulator of chemotaxis, the mechanism through which bacteria respond to changes in the chemical compositions of their environment. In addition to CheY, the N-hydroxyarylamine O-acetyltransferase (NhoA) protein, the citrate synthase (Cs) and the isocitrate dehydrogenase (IcdH) enzymes, both of them belonging to the tricarboxylic acids cycle, and the adenosylmethionine synthase (MetK) are also substrates of CobB sirtuin (Zhang et al, 2013;Sun et al, 2016a;Venkat et al, 2018Venkat et al, , 2019. Recently, the activity and stability of E. coli diguanylate cyclase DgcZ has also been demonstrated to be regulated by deacetylation catalyzed by CobB sirtuin (Xu et al, 2019).…”

Section: Sirtuins In Carbon and Nitrogen Metabolismmentioning

confidence: 99%

Bacterial Sirtuins Overview: An Open Niche to Explore

et al. 2021

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Sirtuins are deacetylase enzymes widely distributed in all domains of life. Although for decades they have been related only to histones deacetylation in eukaryotic organisms, today they are considered global regulators in both prokaryotes and eukaryotes. Despite the important role of sirtuins in humans, the knowledge about bacterial sirtuins is still limited. Several proteomics studies have shown that bacterial sirtuins deacetylate a large number of lysines in vivo, although the effect that this deacetylation causes in most of them remains unknown. To date, only the regulation of a few bacterial sirtuin substrates has been characterized, being their metabolic roles widely distributed: carbon and nitrogen metabolism, DNA transcription, protein translation, or virulence. One of the most current topics on acetylation and deacetylation focuses on studying stoichiometry using quantitative LC-MS/MS. The results suggest that prokaryotic sirtuins deacetylate at low stoichiometry sites, although more studies are needed to know if it is a common characteristic of bacterial sirtuins and its biological significance. Unlike eukaryotic organisms, bacteria usually have one or few sirtuins, which have been reported to have closer phylogenetic similarity with the human Sirt5 than with any other human sirtuin. In this work, in addition to carrying out an in-depth review of the role of bacterial sirtuins in their physiology, a phylogenetic study has been performed that reveals the evolutionary differences between sirtuins of different bacterial species and even between homologous sirtuins.

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“…The LC-MS/MS analyses were performed by Yale University Keck Proteomics Facility following previous protocols (Venkat et al, 2019). The purified ThrRS was digested in gel by trypsin and analyzed by LC-MS/MS on an LTQ Orbitrap XL equipped with a nanoACQUITY UPLC system.…”

Section: Mass and CD Spectrometrymentioning

confidence: 99%

A Synthetic Reporter for Probing Mistranslation in Living Cells

Chen

Ercanbrack

Wang

et al. 2020

Front. Bioeng. Biotechnol.

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Aminoacyl-tRNA synthetases (AARSs) play key roles in maintaining high fidelity of protein synthesis. They charge cognate tRNAs with corresponding amino acids and hydrolyze mischarged tRNAs by editing mechanisms. Impairment of AARS editing activities can reduce the accuracy of tRNA aminoacylation to produce mischarged tRNAs, which cause mistranslation and cell damages. To evaluate the mistranslation rate of threonine codons in living cells, in this study, we designed a quantitative reporter derived from the green fluorescent protein (GFP). The original GFP has multiple threonine codons which could affect the accuracy of measurement, so we generated a GFP variant containing only one threonine residue to specifically quantify mistranslation at the threonine codon. To validate, we applied this single-threonine GFP reporter to evaluate mistranslation at the threonine codon with mutations or modifications of threonine-tRNA synthetase and compared it with other methods of mistranslation evaluation, which showed that this reporter is reliable and facile to use.

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Characterizing lysine acetylation of Escherichia coli type II citrate synthase

Cited by 30 publications

References 42 publications

Poplar acetylome profiling reveals lysine acetylation dynamics in seasonal bud dormancy release

Poplar acetylome profiling reveals lysine acetylation dynamics in seasonal bud dormancy release

Bacterial Sirtuins Overview: An Open Niche to Explore

A Synthetic Reporter for Probing Mistranslation in Living Cells

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