Characterizing microbial communities dedicated for conversion of coal to methane in situ and ex situ

Zhang, Ji; Liang, Yanna; Pandey, Rohit; Harpalani, Satya

doi:10.1016/j.coal.2015.05.001

Cited by 71 publications

(52 citation statements)

References 37 publications

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“…But these cells were not able to produce methane even though archaea strains similar to Methanobrevibacter sp. were present (Zhang et al, 2015); 3) increased CO 2 , but no CH 4 was detected in serum bottles with the inoculum and medium only. For these setups, no coal was provided.…”

Section: Methane Productionmentioning

confidence: 88%

“…Coal samples used in this study were the same as those investigated in another work (Zhang et al, 2015). Briefly, chunks of high volatile B bituminous coals were collected from Herrin Seam (No.…”

Section: Coal Samplesmentioning

confidence: 99%

“…All microcosms were maintained at 28°C in the dark. At days 10, 20 and 30, samples from the headspace in each serum bottle were analyzed by gas chromatography (GC) as described previously (Zhang et al, 2015).…”

Section: Methane Productionmentioning

confidence: 99%

“…Both the original and the adapted consortium contained bacterial and archaeal species and produced methane from coal in a laboratory setting (Zhang et al, 2015). To understand the functionality of the microbial community and the pathways leading to methane from coal, we aimed to identify proteins in anaerobic microcosms designed for bioconversion of coal to methane.…”

Section: Introductionmentioning

confidence: 99%

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A metaproteomic approach for identifying proteins in anaerobic bioreactors converting coal to methane

Zhang

Liang

Yau

et al. 2015

International Journal of Coal Geology

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To understand the processes involved in bioconversion of coal to methane, a metaproteomic approach was taken to identify proteins in microcosms containing coal, standard medium and an adapted microbial community. Concentrated and dialyzed protein samples were subjected to further cleanup and trypsin digestion followed by mass spectrometric analysis. Searching the generated peaklists against domains of bacteria, archaea and fungi revealed 152 ± 1.4, 96.5 ± 2.1 and 38 ± 1.4 protein families, respectively. Proteins associated with bacteria were distributed among transporter and membrane proteins (33.1%), cellular metabolism (28.5%), substrate utilization/conversion (7.3%), oxidative stress (5.3%), cell movement (3.3%) and hypothetical proteins (22.5%). Among the total archaea proteins, 37.8% were for substrate utilization related to methane production, 27.6% were for cellular metabolism, 6.1% responded to stress, 5.1% were transporter and membrane proteins and 23.5% were those with unknown functions. Proteins produced by fungi fell in two groups: cell metabolisms (45.7%) and hypothetical proteins (54.3%). Based on key enzymes identified, a pathway for methanogenesis in the tested samples was proposed. This pathway illustrated methane production from four starting compounds, acetate, formate, methanol and CO 2 . The proposed pathway will serve as a solid foundation for future effort aiming to increase methane yield from coal.Published by Elsevier B.V.

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Section: Methane Productionmentioning

confidence: 88%

Section: Coal Samplesmentioning

confidence: 99%

Section: Methane Productionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A metaproteomic approach for identifying proteins in anaerobic bioreactors converting coal to methane

Zhang

Liang

Yau

et al. 2015

International Journal of Coal Geology

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“…The study by Zhang et al (2015) was successful in formulating methods to produce methane from coal by simulating methanogenesis using suitable nutrient amendments. From a CBM perspective, wells which are depleted or nearing depletion, can be treated with suitable microbial consortia.…”

Section: Discussionmentioning

confidence: 99%

Changes in properties of coal as a result of continued bioconversion

Pandey¹

2016

International Conference and Exhibition, Barcelona, Spain, 3-6 April 2016

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Microbial actions on coal have long been identified as a source of methane in coalbeds.Andrew Scott (1995) was the first to propose imitating the natural process of biogenic gasification, possibly leading to recharging coalbed methane (CBM) reservoirs, or setting up natural gas reservoirs in non-producing coalbeds. This study was aimed at identifying the changes in coal properties that affect gas deliverability in coal-gas reservoirs, when treated with microbial consortia to generate/enhance gas production. The experimental work tested the sorption and diffusion properties for the coal treated and, more importantly, the variation in the relevant parameters with continued bio-conversion since these are the first two phenomena in CBM production.During the first phase, single component sorption-diffusion experiments were carried out using pure methane and CO2 on virgin/baseline coals, retrieved from the Illinois basin. Coals were then treated with nutrient amended microbial consortia for different periods. Gas production was monitored at the end of thirty and sixty days of treatment, after which, sorption-diffusion experiments were repeated on treated coals, thus establishing a trend over the sixty-day period.The sorption data was characterized using Langmuir pressure and volume constants, obtained by fitting it over the Langmuir isotherm. The diffusion coefficient, D, was estimated by establishing ii the variation trend as a function of pore pressure. The pressure parameter was considered critical since, with continued production of methane, the produced gas diffuses into the coal matrix, where it gets adsorbed with increasing pressure. During production, the pressure decreases and the process is reversed, gas diffusing out of the coal matrix and arriving at the cleat system.The results indicated an increase in the sorption capacity of coal as a result of bioconversion. This was attributed to increased pore surface areas as a result of microbial actions. However, significant hysteresis was observed during desorption of methane and was attributed to preferential desorption from sorption sites in the pathways leading to pore cavities. This is corroborated by the increased rates of diffusion, especially for methane, which exhibited rates higher than that for CO2. This contradicted the results for untreated/baseline coal, which were in agreement with previous studies. Effort was made to explain this anomaly by the nonmonotonic dependence of effective diffusion coefficient on the size of the diffusing particles, where in coalbed environments, CO2 has smaller kinetic diameter than methane.iii ACKNOWLEDGMENTS I would like to express my sincere appreciation and gratitude to my academic advisor and Thesis Chair, Dr. Satya Harpalani, for his guidance, encouragement, constructive criticism and patience throughout my study. Without his help and advice, this study would not have been possible. I would also like to thank him for giving me the opportunity to come to Southern Illinois University and work under his supervision.

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Single-chamber microbial electrochemical cell for CH₄production from CO₂utilizing a microbial consortium

et al. 2017

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Summary We report the first single‐chamber microbial electrochemical cell for conversion of CO2 to CH4, with an average CH4 production rate of 0.47 ± 0.05 mL day−1 cm−2 at an applied potential of 600 mV, utilizing a methanogenic microbial community collected from the formation water in the San Juan coal basin (Colorado, USA). CH4 production was only observed at the graphite rod cathode after an electrochemical pre‐treatment that facilitates biofilm formation. The carbon contained within the CH4 arose predominantly from the CO2 source, as verified by experiments during which the CO2 source was repeatedly turned off and on. Modest quantities of acetic acid and ethanol were also produced. DNA extraction and sequencing from the microbial community showed that from the Archaea kingdom, only 2 species survived prolonged exposure to CO2 and CH4 production, methanobacterium sp. (81.4%), and methanoculleus sp. (18.6%), while in the bacterial kingdom, anaerobaculum thermoterrnum (67.1%) was the predominant surviving species.

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Characterizing microbial communities dedicated for conversion of coal to methane in situ and ex situ

Cited by 71 publications

References 37 publications

A metaproteomic approach for identifying proteins in anaerobic bioreactors converting coal to methane

A metaproteomic approach for identifying proteins in anaerobic bioreactors converting coal to methane

Changes in properties of coal as a result of continued bioconversion

Single-chamber microbial electrochemical cell for CH₄production from CO₂utilizing a microbial consortium

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Characterizing microbial communities dedicated for conversion of coal to methane in situ and ex situ

Cited by 71 publications

References 37 publications

A metaproteomic approach for identifying proteins in anaerobic bioreactors converting coal to methane

A metaproteomic approach for identifying proteins in anaerobic bioreactors converting coal to methane

Changes in properties of coal as a result of continued bioconversion

Single-chamber microbial electrochemical cell for CH4production from CO2utilizing a microbial consortium

Contact Info

Product

Resources

About

Single-chamber microbial electrochemical cell for CH₄production from CO₂utilizing a microbial consortium