Background: Phosphorylation of phospholamban regulates cardiac calcium transport, but the content and function of the four phosphorylation states of phospholamban are unknown. Results: Synthetic phosphopeptides solved both problems.
Conclusion:The phosphorylation states were quantified in normal and hypertrophic pig hearts, and each has a distinct effect on calcium transport. Significance: This information is needed for improved diagnosis and treatment of heart failure.