Characterizing property and treatability of dissolved effluent organic matter using size exclusion chromatography with an array of absorbance, fluorescence, organic nitrogen and organic carbon detectors

Sustainable Treatment and Resource Recovery of Anion Exchange Spent Brine by Pilot-Scale Electrodialysis and Ultrafiltration

“…The SEC–DAD–FLD–OCD analyses of AIX spent brine, first-stage concentrate solution, second-stage dilute solution and second-stage concentrate solution to study the transport of organic substances through IEMs during the two-stage pilot-scale ED process are shown in Figure 4 . According to previous research [ 23 , 24 ], the DAD measurements (the wavelength range > 240 nm) characterize components that respond to UV absorbance, especially the aromatic and unsaturated functional groups in NOM. The FLD detector with multiple emission wavelength scanning identified humic-like substances at Em > 380 nm and protein-like substances at Em < 380 nm [ 38 ].…”

“…They exhibited strong humic-like fluorescence peaks in the FLD chromatogram and intensive UV absorbance at wavelengths >240 nmin the DAD chromatogram, shown in Figure 4 a. This reflected the presence of aromatic moieties typical for HSs [ 23 ]. The peak value of HSs was<600 mg/Lin the OCD chromatogram, for which apparent molecular weight (AMW) was located at ~7.2 kDa to ~1.3 kDa.…”

“…BP has been reported as mainly being polysaccharides, acromolecular proteins and amino acids [ 24 ]. The molecular weight of BB was relatively small, and it might be degradation products of humic substances as well as made up of both aromatic and aliphatic low molecular weight acids [ 23 , 24 ]. LMWS was mainly composed of low molecular weight neutrals.…”

“…Detailed experimental conditions of two-stage pilot-scale ED are available in the Supplementary Material . The SEC–DAD–FLD–OCD systems [ 23 , 24 ], which are described in detail in the Supplementary Material , were used to measure the properties of AIX spent brine, second-stage dilute solution, first-stage concentrate solution, and second-stage concentrate solution to study the transport of organic matters through the ion exchange membranes (IEMs) in the two-stage pilot-scale ED process. Dilute solution conductivity and concentrate solution conductivity were determined in the pilot-scale ED experiment process.…”

Identification of Extracellular Key Enzyme and Intracellular Metabolic Pathway in Alginate-Degrading Consortia via an Integrated Metaproteomic/Metagenomic Analysis

“…The absorbance change of alginate at UV 254 nm was determined by a UV–visible spectrophotometer (A560, AOE Instruments, CN). Profiles of alginate and hydrolysate at UV 254 nm were measured by a high-performance liquid chromatography system (Agilent 1260, USA) . The m / z values of alginate hydrolysate after dosing alginate lyase on day 4 were analyzed by MALDI-TOF-MS (Ultraflextreme, Bruker, GER).…”