Characterizing the dual-wavelength dye indo-1 for calcium-ion sensing under pressure

Ryan, Jordan; Urayama, Paul

doi:10.1039/c1ay05486d

Cited by 3 publications

(3 citation statements)

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“…Those values are comparable to those that have been measured for metal ion chelators and other calcium sensitive fluorescent dyes. (Hasselbach et al[ 15 ] determined the calcium binding reaction volume of Ca 2+ and EDTA to be 17.8 ml mol -1 and Ryan et al[ 14 ] described the dissociation volume of Calcium from Indo-1 to be -23.5 ml mol -1 )…”

Section: Resultsmentioning

confidence: 99%

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Calcium Sensitive Fluorescent Dyes Fluo-4 and Fura Red under Pressure: Behaviour of Fluorescence and Buffer Properties under Hydrostatic Pressures up to 200 MPa

et al. 2016

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The fluorescent Ca2+ sensitive dyes Fura Red (ratiometric) and Fluo-4 (non-ratiometric) are widely utilized for the optical assessment of Ca2+ fluctuations in vitro as well as in situ. The fluorescent behavior of these dyes is strongly depends on temperature, pH, ionic strength and pressure. It is crucial to understand the response of these dyes to pressure when applying calcium imaging technologies in the field of high pressure bioscience. Therefore, we use an optically accessible pressure vessel to pressurize physiological Ca2+-buffered solutions at different fixed concentrations of free Ca2+ (1 nM to 25.6 μM) and a specified dye concentration (12 μM) to pressures of 200 MPa, and record dye fluorescence intensity. Our results show that Fluo-4 fluorescence intensity is reduced by 31% per 100 MPa, the intensity of Fura Red is reduced by 10% per 100 MPa. The mean reaction volume for the dissociation of calcium from the dye molecules is determined to -17.8 ml mol-1 for Fluo-4 and -21.3 ml mol-1 for Fura Red. Additionally, a model is presented that is used to correct for pressure-dependent changes in pH and binding affinity of Ca2+ to EGTA, as well as to determine the influence of these changes on dye fluorescence.

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Section: Resultsmentioning

confidence: 99%

“…Under isothermal equilibrium conditions and discretising the change in pressure, Eq (10) reduces to Eq (11) [ 14 ]. The equation is, in principle, similar to the isobaric Arrhenius-equation but describes the pressure-induced change in reaction equilibrium under isothermal conditions.…”

Section: Methodsmentioning

confidence: 99%

Calcium Sensitive Fluorescent Dyes Fluo-4 and Fura Red under Pressure: Behaviour of Fluorescence and Buffer Properties under Hydrostatic Pressures up to 200 MPa

et al. 2016

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“…Some optical sensors utilize a potential sensitive dye (PSD) that changes its optical properties in response to microenvironment changes (Mohr et al, 1997). Research has been conducted to develop optical sensors for various ions, such as nitrate (Mohr et al, 1995), nitrite (Mohr and Wolfbeis, 1996), chloride (Huber et al, 1999), ammonium (Stromberg and Hulth, 2005), and calcium (Ryan and Urayama, 2012). Most of these optical sensors used tridodecylmethyl ammonium chloride (TDMACl) as an ion carrier.…”

Section: Introductionmentioning

confidence: 99%

Disposable Nitrate-Selective Optical Sensor Based on Fluorescent Dye

Kim¹,

Sudduth²,

Grant³

et al. 2012

Journal of Biosystems Engineering

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Purpose: This study was performed to develop a simple, disposable thin-film optical nitrate sensor. Methods: The sensor was fabricated by applying a nitrate-selective polymer membrane on the surface of a thin polyester film. The membrane was composed of polyvinylchloride (PVC), plasticizer, fluorescent dye, and nitrate-selective ionophore. Fluorescence intensity of the sensor increased on contact with a nitrate solution. The fluorescence response of the optical nitrate sensor was measured with a commercial fluorospectrometer. Results: The optical sensor exhibited linear response over four concentration decades. Conclusions: Nitrate ion concentrations in plant nutrient solutions can be determined by direct optical measurements without any conditioning before measurements.

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Advancements in Cellular Imaging: Expanding Horizons with Innovative Dyes and Techniques

Oak,

Mali

2024

Colorants

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Advancements in cellular imaging have significantly enhanced our understanding of membrane potential and Ca2⁺ dynamics, which are crucial for various cellular processes. Voltage-sensitive dyes (VSDs) are pivotal in this field, enabling non-invasive, high-resolution visualization of electrical activity in cells. This review discusses the various types of VSDs, including electrochromic, Förster Resonance Energy Transfer (FRET)-based, and Photoinduced Electron Transfer (PeT)-based dyes. VSDs are essential tools for studying mitochondrial activity and neuronal function and are frequently used in conjunction with Ca2⁺ indicators to elucidate the complex relationship between membrane potential and Ca2⁺ fluxes. The development of novel dyes with improved photostability and reduced toxicity continues to expand the potential of VSDs in biomedical research. This review underscores the importance of VSDs in advancing our understanding of cellular bioenergetics, signaling, and disease mechanisms.

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Characterizing the dual-wavelength dye indo-1 for calcium-ion sensing under pressure

Cited by 3 publications

References 23 publications

Calcium Sensitive Fluorescent Dyes Fluo-4 and Fura Red under Pressure: Behaviour of Fluorescence and Buffer Properties under Hydrostatic Pressures up to 200 MPa

Calcium Sensitive Fluorescent Dyes Fluo-4 and Fura Red under Pressure: Behaviour of Fluorescence and Buffer Properties under Hydrostatic Pressures up to 200 MPa

Disposable Nitrate-Selective Optical Sensor Based on Fluorescent Dye

Advancements in Cellular Imaging: Expanding Horizons with Innovative Dyes and Techniques

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