Characterizing the Hematologic and Plasma Chemistry Profiles of Captive Crested Geckos (Rhacodactylus ciliatus)

Mayer, Jörg; Knoll, Joyce S.; Wrubel, Kathryn M.; Mitchell, Mark A.

doi:10.5818/1529-9651-21.2.68

Cited by 17 publications

(14 citation statements)

References 8 publications

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“…One study found no differences between the PCVs of male and female Asian water dragons, but another found that female green iguanas ( Iguana iguana ) had higher PCVs than males and a third study reported that RBC numbers are higher in males than females . Mayer et al more recently reported higher PCVs in captive male crested geckoes ( Rhacodactylus ciliatus ) compared with females . We identified no significant sex differences in RBC number or PCV in this study.…”

Section: Discussionsupporting

confidence: 49%

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Baseline morphometric, haematological and plasma biochemical parameters in free‐ranging eastern water dragons (Intellagama lesueurii lesueurii)

et al. 2018

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Objective The aim of this study was to determine baseline reference data for morphometric measurements and haematological and plasma biochemical parameters in clinically healthy eastern water dragons (Intellagama lesueurii lesueurii), accounting for the variables of season and sex. The clinical objective was to provide clinicians and researchers with baseline reference intervals (RIs) in order to assess accurately the health of a population or individual animals. Methods The study group comprised 39 free‐ranging eastern water dragons. To monitor seasonal changes in data two study periods were assigned: September–October 2009 (spring) and January–March 2010 (summer). The 18 males and 21 females were captured in the grounds of Taronga Zoo, Mosman (33°50′45′′ S, 151°15′20′′ E) in Sydney, New South Wales, Australia. Basic morphometric, haematological and plasma biochemical parameters were measured. RIs were generated in accordance with the American Society of Veterinary Clinical Pathology guidelines. Data were analysed for the effects of sex, season and sex–season interactions using restricted maximal likelihood modelling. Results Reference values were established in eastern water dragons for morphometric data and RIs were generated for haematological and plasma biochemical levels. The study showed seasonal variation in uric acid. Weight, total length, snout–vent length and basophil % showed significant differences according to sex. Sex–season interactions were detected for calcium, monocyte % and total protein (refractometry). Conclusion Multiple parameters exhibited normal physiological variation between season and sex and an interaction between the two factors. These variables need to be considered in the clinical context when interpreting results of haematological and biochemical analyses in eastern water dragons. The RIs established from this population will provide a basis for further studies in both free‐living and captive eastern water dragons, and for comparison in clinical cases.

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Section: Discussionsupporting

confidence: 49%

“…9 Mayer et al more recently reported higher PCVs in captive male crested geckoes (Rhacodactylus ciliatus) compared with females. 31 We identified no significant sex differences in RBC number or PCV in this study. The RI for PCV in reptiles is 20-40%, depending upon species, and plasma protein intervals range from 3 to 8 g/dL.…”

Section: Discussionmentioning

confidence: 53%

Baseline morphometric, haematological and plasma biochemical parameters in free‐ranging eastern water dragons (Intellagama lesueurii lesueurii)

et al. 2018

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“…Complete blood count, plasma biochemistry profile and faecal examinations were also performed. Venipuncture was performed under isoflurane sedation with an insulin syringe with a 27‐gauge needle either in the ventral coccygeal vein using a lateral approach or in the cranial vena cava as needed to avoid autotomy, depending on the temperament of the individuals, as previously described in geckos (Gould et al., ; Mayer, Knoll, Wrubel, & Mitchell, ). A maximum blood volume of 0.5 ml, corresponding to maximum 1% of the body weight, was sampled.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of β‐carotene assimilation in leopard geckos (Eublepharis macularius)

Cojean

Lair

Vergneau‐Grosset

2018

Animal Physiology Nutrition

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Although leopard geckos (Eublepharis macularius) are commonly kept under human care, their vitamin requirements are largely unknown. Many invertebrate preys display a low vitamin A concentration; thus, gut-loading insects with vitamin A or carotenoids is a common practice. The objective of this prospective experimental study was to investigate whether dietary supplementation with β-carotene, including prey gut-loading, leads to sufficient vitamin A hepatic storage and prevents epithelial squamous metaplasia development in leopard geckos. Ten clinically healthy female leopard geckos were randomly divided in two groups with various supplementations: a group receiving vitamin A supplementation and a group receiving β-carotene. Insects were gut-loaded continuously with a supplement containing vitamin A or β-carotene, depending on the group. Oral supplementation with cod liver oil or carrot juice was administered weekly to each lizard from "vitamin A group" and "carotenoid group" respectively. After 10 weeks of supplementation, surgical hepatic biopsies were obtained in three geckos of each group while the two remaining geckos were euthanized to undergo complete necropsy. Hepatic vitamin A concentration was determined for each lizard (n = 10) by ultra-performance liquid chromatography. Histopathology revealed hepatocellular vacuolization and vitellogenic follicles in five females. Epithelial squamous metaplasia was not observed in any of the geckos. Hepatic vitamin A concentration was significantly higher in the carotenoid-supplemented group than in the vitamin A-supplemented group (p = 0.03). Our results suggest that in leopard geckos, dietary supplementation with β-carotene allows sufficient vitamin A hepatic storage.

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“…2011;Olayemi 2011;Salamat et al 2013). Results of previous studies showed variations, which were due to animal selection and the methods used for venepuncture and the processing of blood samples (Redrobe andMacDonald 1999, Pejrilova et al 2004;Hernandez-Divers 2006;Knotkova et al 2005;Knotkova et al 2008;Mayer et al 2011). Blood collection from the ventral tail vein is difficult and can potentially lead to caudal autotomy.…”

mentioning

confidence: 99%

“…The leopard gecko (Eublepharis macularius) is one of the most common species of captive lizards and the most traded species of the family Eublepharidae. Blood analysis in geckos has been a subject of interest for some authors (Sacchi et al 2007;Mayer at al. 2011;Olayemi 2011;Salamat et al 2013).…”

mentioning

confidence: 99%

Blood profile in captive adult male leopard geckos (Eublepharis macularius)

Knotková¹,

Morici²,

Oliveri³

et al. 2019

Vet. Med.

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The aim of this study was to determine blood profile data in captive adult male leopard geckos. Animals were manually restrained with the head and neck extended. The right external jugular vein was punctured with a pre-heparinised needle and insulin syringe. The means and standard deviations for haemoglobin concentration, packed cell volume, total red blood cell count, total white blood cell count and counts for heterophils, basophils, eosinophils, monocytes, azurophils and lymphocytes for 20 healthy male leopard geckos were 72.58 ± 11.03 g/l, 25.40 ± 3.68%, 0.85 ± 0.14 1012/l, 10.47 ± 2.58 109/l, 1.83 ± 0.92 109/l, 1.67 ± 1.04 109/l, 0.29 ± 0.33 109/l, 0.48 ± 0.40 109/l, 2.03 ± 1.07 109/l and 4.17 ± 2.12 109/l, respectively. The means and standard deviations for total protein, albumin, globulins, glucose, uric acid, aspartate aminotransferase, creatine kinase, calcium, phosphorus and potassium for 20 healthy adult captive male leopard geckos were 55.60 ± 7.52 g/l, 16.45 ± 2.37 g/l, 39.15 ± 5.74 g/l, 6.18 ± 1.35 mmol/l, 67.95 ± 42.63 µmol/l, 0.83 ± 0.42 µkat/l, 25.40 ± 29.46 µkat/l, 3.05 ± 0.18 mmol/l, 1.4 ± 0.23 mmol/l, and 5.78 ± 0.58 mmol/l, respectively. This is the first study to report blood haematology and biochemistry values for a group of captive adult male leopard geckos.

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Characterizing the Hematologic and Plasma Chemistry Profiles of Captive Crested Geckos (Rhacodactylus ciliatus)

Cited by 17 publications

References 8 publications

Baseline morphometric, haematological and plasma biochemical parameters in free‐ranging eastern water dragons (Intellagama lesueurii lesueurii)

Baseline morphometric, haematological and plasma biochemical parameters in free‐ranging eastern water dragons (Intellagama lesueurii lesueurii)

Evaluation of β‐carotene assimilation in leopard geckos (Eublepharis macularius)

Blood profile in captive adult male leopard geckos (Eublepharis macularius)

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