Characterizing the Role of TaWRKY13 in Salt Tolerance

Zhou, Shuo; Zheng, Wenbin; Liu, Baohua; Zheng, Jingwu; Dong, Fang; Liu, Zhifang; Wen, Zhi-Yu; Yang, Fan; Wang, Haibo; Xu, Zhao‐Shi; He, Zhao; Yongwei, Liu

doi:10.3390/ijms20225712

Cited by 22 publications

(8 citation statements)

References 63 publications

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“…In addition, TaWRKY4, 8, 6 had the highest numbers of upregulated genotypes. Our findings are consistent with previous results reporting the expression levels of TaWRKY44 and TaWRKY4 [58], TaWRKY93 [59], and TaWRKY7 and TaWRKY12 [16] in plants exposed to salinity. In contrast, TaWRKY2, 20 had the lowest numbers of upregulated genotypes.…”

Section: Discussionsupporting

confidence: 93%

Detection of genetic divergence among some wheat (Triticum aestivum L.) genotypes using molecular and biochemical indicators under salinity stress

Gowayed

El-Moneim

2021

PLoS ONE

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Wheat has remarkable importance among cereals in Egypt. Salt stress affects plant growth, development, and crop productivity. Therefore, salinity tolerance is an essential trait that must be incorporated in crops. This research aimed to investigate molecular and biochemical indicators and defence responses in seedlings of 14 Egyptian wheat genotypes to distinguish the most contrasting salt-responsive genotypes. Analysis of ISSR and SCoT markers revealed high polymorphism and reproducible fingerprinting profiles for evaluating genetic variability within the studied genotypes. The HB-10 and SCoT 1 primers had the highest values for all the studied parameters. All the tested primers generated a set of 66 polymorphic bands among tolerant and sensitive genotypes. The transcript profiles of eight TaWRKY genes showed significant induction under the salinity treatments. Moreover, the expression of TaWRKY6 for genotypes Sids 14 and Sakha 93 sharply increased and recorded the highest expression, while the expression of TaWRKY20 for Misr 1 recorded the lowest expression. Under salt stress, the total sugar, proline, and phenolic contents increased significantly, while the chlorophyll content decreased significantly. Additionally, five peroxidase and polyphenol oxidase isoforms were observed in treated leaves and clustered into five different patterns. Some isoforms increased significantly as salinity levels increased. This increase was clearer in salt-tolerant than in salt-sensitive genotypes. Eighteen protein bands appeared, most of which were not affected by salinity compared with the control, and specific bands were rare. Generally, the Sids 14, Sakha 93, Sohag 4, and Gemmeiza 12 genotypes are considered salt tolerant in comparison to the other genotypes.

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Section: Discussionsupporting

confidence: 93%

Detection of genetic divergence among some wheat (Triticum aestivum L.) genotypes using molecular and biochemical indicators under salinity stress

Gowayed

El-Moneim

2021

PLoS ONE

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“…SbWRKY30 enhanced the drought tolerance of sorghum by regulating the drought stress response gene SbRD19 [74]. The overexpression of the wheat WRKY transcription factor gene TaWRKY13 can significantly improve salt tolerance in rice, indicating that the WRKY transcription factor was a positive factor responding to salt stress [75].…”

Section: Analysis Of Differential Gene Expressionmentioning

confidence: 99%

De novo transcriptome sequencing and analysis of salt-, alkali-, and drought-responsive genes in Sophora alopecuroides

et al. 2020

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Background: Salinity, alkalinity, and drought stress are the main abiotic stress factors affecting plant growth and development. Sophora alopecuroides L., a perennial leguminous herb in the genus Sophora, is a highly salt-tolerant sand-fixing pioneer species distributed mostly in Western Asia and northwestern China. Few studies have assessed responses to abiotic stress in S. alopecuroides. The transcriptome of the genes that confer stress-tolerance in this species has not previously been sequenced. Our objective was to sequence and analyze this transcriptome. Results: Twelve cDNA libraries were constructed in triplicate from mRNA obtained from Sophora alopecuroides for the control and salt, alkali, and drought treatments. Using de novo assembly, 902,812 assembled unigenes were generated, with an average length of 294 bp. Based on similarity searches, 545,615 (60.43%) had at least one significant match in the Nr, Nt, Pfam, KOG/COG, Swiss-Prot, and GO databases. In addition, 1673 differentially expressed genes (DEGs) were obtained from the salt treatment, 8142 from the alkali treatment, and 17,479 from the drought treatment. A total of 11,936 transcription factor genes from 82 transcription factor families were functionally annotated under salt, alkali, and drought stress, these include MYB, bZIP, NAC and WRKY family members. DEGs were involved in the hormone signal transduction pathway, biosynthesis of secondary metabolites and antioxidant enzymes; this suggests that these pathways or processes may be involved in tolerance towards salt, alkali, and drought stress in S. alopecuroides. Conclusion: Our study first reported transcriptome reference sequence data in Sophora alopecuroides, a non-model plant without a reference genome. We determined digital expression profile and discovered a broad survey of unigenes associated with salt, alkali, and drought stress which provide genomic resources available for Sophora alopecuroides.

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“…Also, Arabidopsis plants heterologously expressing the maize ( Zea mays ) ZmWRKY40 gene exhibited improved tolerance to drought [ 12 ]. Similarly, heterologous expression of TaWRKY13 in Arabidopsis increased root length and proline content, and reduced malondialdehyde content, thus improving salt stress tolerance [ 13 ]. Overexpression of WRKY13 in Arabidopsis enhanced cadmium tolerance of transgenic plants by inducing the expression of PLEIOTROPIC DRUG RESISTANCE 8 ( PDR8 ), encoding an ATP-binding cassette transporter [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide Identification of WRKY transcription factor family members in sorghum (Sorghum bicolor (L.) moench)

et al. 2020

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WRKY transcription factors regulate diverse biological processes in plants, including abiotic and biotic stress responses, and constitute one of the largest transcription factor families in higher plants. Although the past decade has seen significant progress towards identifying and functionally characterizing WRKY genes in diverse species, little is known about the WRKY family in sorghum ( Sorghum bicolor (L.) moench). Here we report the comprehensive identification of 94 putative WRKY transcription factors ( Sb WRKYs). The Sb WRKYs were divided into three groups (I, II, and III), with those in group II further classified into five subgroups (IIa–IIe), based on their conserved domains and zinc finger motif types. WRKYs from the model plant Arabidopsis ( Arabidopsis thaliana ) were used for the phylogenetic analysis of all SbWRKY genes. Motif analysis showed that all Sb WRKYs contained either one or two WRKY domains and that Sb WRKYs within the same group had similar motif compositions. SbWRKY genes were located on all 10 sorghum chromosomes, and some gene clusters and two tandem duplications were detected. SbWRKY gene structure analysis showed that they contained 0–7 introns, with most SbWRKY genes consisting of two introns and three exons. Gene ontology (GO) annotation functionally categorized SbWRKYs under cellular components, molecular functions and biological processes. A cis -element analysis showed that all SbWRKYs contain at least one stress response-related cis -element. We exploited publicly available microarray datasets to analyze the expression profiles of 78 SbWRKY genes at different growth stages and in different tissues. The induction of SbWRKYs by different abiotic stresses hinted at their potential involvement in stress responses. qRT-PCR analysis revealed different expression patterns for SbWRKYs during drought stress. Functionally characterized WRKY genes in Arabidopsis and other species will provide clues for the functional characterization of putative orthologs in sorghum. Thus, the present study delivers a solid foundation for future functional studies of SbWRKY genes and their roles in the response to critical stresses such as drought.

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Characterizing the Role of TaWRKY13 in Salt Tolerance

Cited by 22 publications

References 63 publications

Detection of genetic divergence among some wheat (Triticum aestivum L.) genotypes using molecular and biochemical indicators under salinity stress

Detection of genetic divergence among some wheat (Triticum aestivum L.) genotypes using molecular and biochemical indicators under salinity stress

De novo transcriptome sequencing and analysis of salt-, alkali-, and drought-responsive genes in Sophora alopecuroides

Genome-wide Identification of WRKY transcription factor family members in sorghum (Sorghum bicolor (L.) moench)

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