Characterizing the yielding processes in pluronic-hyaluronic acid thermoreversible gelling systems using oscillatory rheology

Ramya, Koduvayur A.; Kodavaty, Jagadeeshwar; Dorishetty, Pramod; Setti, Mahesh; Deshpande, Abhijit P.

doi:10.1122/1.5045073

Cited by 21 publications

(11 citation statements)

References 61 publications

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“…In all systems, the correlation coefficient exhibited fast exponential decay and a single-mode characteristic at a low temperature of 20 °C ( Figure 2 a, sol state), while slow multi-mode relaxation appeared at a corresponding gel temperature of 35 °C ( Figure 2 b, inter-micellar aggregates and arrange to gel state). This result is in agreement with previous findings that PF127 is soluble in aqueous solution at low temperatures and forms a gel with increasing temperature [ 20 , 47 ].…”

Section: Resultssupporting

confidence: 94%

“…Extensive research has been conducted on diverse types of pluronic hydrogels that could serve as a base owing to their thermosensitivity. To overcome their limitations of weak mechanical properties and swift erosion under physiological conditions, researchers have used chemical crosslinking or the formation of physical mixtures by blending in proteins, polysaccharides, and semi-interpenetrating polymeric networks (SIPNs) [ 19 , 20 ]. Thus, hydrogels consisting of a physical mixture of pluronic and hyaluronic acid (HA) [ 21 , 22 ], HA chemically grafted onto pluronic [ 23 ], a combination of alginate, pluronic, and HA [ 24 ], or chemically grafted or physically blended alginate and pluronic [ 25 , 26 ] could exhibit gelation behavior and serve as vehicles for long-term delivery of drugs or cells.…”

Section: Introductionmentioning

confidence: 99%

“…The objective of this study was to develop a novel, smart, reversible-response IPN hydrogel as a functional wound dressing with pro-angiogenic activity to accelerate wound healing. Briefly, from the pluronic series of polymers, nonionic PF127 was selected as the basis of the hydrogel system because of its simple phase behavior and low gelation concentration [ 20 ]. We used both alginate (because of its biocompatibility) and PF127 to develop an IPN (PF127/SA) hydrogel platform that would release its drug payload for wound treatment.…”

Section: Introductionmentioning

confidence: 99%

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Design of an Interpenetrating Polymeric Network Hydrogel Made of Calcium-Alginate from a Thermos-Sensitive Pluronic Template as a Thermal-Ionic Reversible Wound Dressing

et al. 2020

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Polymer-based hydrogels demonstrate superior performance when used as wound dressing. An ideal dressing should possess an active healing function, absorb wound exudates, and provide a moist interface on the wound for rapid injury repair and the prevention of pain and injury during replacement of the dressing. Thus, the aim of this study was to develop a novel, reversible, smart, interpenetrating polymeric network (IPN) by utilizing the thermosensitive network of pluronic F127 (PF127) as a template to regulate the conformation of calcium-ion-crosslinked alginate. We found that the IPN hydrogels formed soft and elastic thermosensitive networks, retaining their form even after absorbing a large amount of wound exudate. The exterior of the hydrogels was made up of a rigid calcium alginate network that supported the entire hydrogel, promoting the stability of the vascular endothelial growth factor (VEGF) payload and controlling its release when the hydrogel was applied topically to wounds. Raman spectroscopy confirmed the layered structure of the hydrogel, which was found to easily disintegrate even after moderate rinsing of the wound with cold phosphate-buffered saline. Taken together, these results show that the IPN hydrogel developed in this study could be a promising delivery platform for growth factors to accelerate wound healing.

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Section: Resultssupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Design of an Interpenetrating Polymeric Network Hydrogel Made of Calcium-Alginate from a Thermos-Sensitive Pluronic Template as a Thermal-Ionic Reversible Wound Dressing

et al. 2020

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“…In the past decade, LAOS has become a canonical technique for quantifying the rheological characteristics of polymers, soft solids, gels, emulsions and various other complex fluids and materials 48,54 . The techniques have also been successfully applied to understand the intracycle strain dependent hardening/softening or thickening/ thinning of biological materials, examples of which include: mucus of the gastropod 55 , hagfish slime 56 , fibrobalst cells 57 , fibrin/collagen gels 58 , vocal fold tissues 59 , pluronic/hyaluronic acid 60,61 , Xanthan gum 62 and blood 63 . For biofilms, LAOS provides us with the ability to determine the nonlinear material response of biofilms when subjected to large shear.…”

Section: Introductionmentioning

confidence: 99%

Nonlinear rheological characteristics of single species bacterial biofilms

Jana

Charlton

Eland

et al. 2020

npj Biofilms Microbiomes

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Bacterial biofilms in natural and artificial environments perform a wide array of beneficial or detrimental functions and exhibit resistance to physical as well as chemical perturbations. In dynamic environments, where periodic or aperiodic flows over surfaces are involved, biofilms can be subjected to large shear forces. The ability to withstand these forces, which is often attributed to the resilience of the extracellular matrix. This attribute of the extracellular matrix is referred to as viscoelasticity and is a result of selfassembly and cross-linking of multiple polymeric components that are secreted by the microbes. We aim to understand the viscoelastic characteristic of biofilms subjected to large shear forces by performing Large Amplitude Oscillatory Shear (LAOS) experiments on four species of bacterial biofilms: Bacillus subtilis, Comamonas denitrificans, Pseudomonas fluorescens and Pseudomonas aeruginosa. We find that nonlinear viscoelastic measures such as intracycle strain stiffening and intracycle shear thickening for each of the tested species, exhibit subtle or distinct differences in the plot of strain amplitude versus frequency (Pipkin diagram). The biofilms also exhibit variability in the onset of nonlinear behaviour and energy dissipation characteristics, which could be a result of heterogeneity of the extracellular matrix constituents of the different biofilms. The results provide insight into the nonlinear rheological behaviour of biofilms as they are subjected to large strains or strain rates; a situation that is commonly encountered in nature, but rarely investigated.

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“…At stage i, close to the strain extremes, we observed isotropic scattering patterns with negligible alignment that is characteristic of the quiescent structure. The phenomenology that linear viscoelasticity and quiescent structure occur at the strain extreme, has been reported by several groups studying a range of soft materials [43,55,56,57,58,59]. This behavior has recently been rationalized [28] by invoking the concept of recoverable strain.…”

Section: Resultsmentioning

confidence: 97%

Unveiling Temporal Nonlinear Structure–Rheology Relationships under Dynamic Shearing

2019

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Understanding how microscopic rearrangements manifest in macroscopic flow responses is one of the central goals of nonlinear rheological studies. Using the sequence-of-physical-processes framework, we present a natural 3D structure–rheology space that temporally correlates the structural and nonlinear viscoelastic parameters. Exploiting the rheo-small-angle neutron scattering (rheo-SANS) techniques, we demonstrate the use of the framework with a model system of polymer-like micelles (PLMs), where we unveil a sequence of microscopic events that micelles experience under dynamic shearing across a range of frequencies. The least-aligned state of the PLMs is observed to migrate from the total strain extreme toward zero strain with increasing frequency. Our proposed 3D space is generic, and can be equally applied to other soft materials under any sort of deformation, such as startup shear or uniaxial extension. This work therefore provides a natural approach for researchers to study complex out-of-equilibrium structure–rheology relationships of soft materials.

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Characterizing the yielding processes in pluronic-hyaluronic acid thermoreversible gelling systems using oscillatory rheology

Cited by 21 publications

References 61 publications

Design of an Interpenetrating Polymeric Network Hydrogel Made of Calcium-Alginate from a Thermos-Sensitive Pluronic Template as a Thermal-Ionic Reversible Wound Dressing

Design of an Interpenetrating Polymeric Network Hydrogel Made of Calcium-Alginate from a Thermos-Sensitive Pluronic Template as a Thermal-Ionic Reversible Wound Dressing

Nonlinear rheological characteristics of single species bacterial biofilms

Unveiling Temporal Nonlinear Structure–Rheology Relationships under Dynamic Shearing

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