Charge-biased nanofibrous membranes with uniform charge distribution and hemocompatibility for enhanced selective adsorption of endotoxin from plasma

Dou, Weidong; Qi, Feng; Li, Yangyang; Wei, Fangfang; Hu, Qitao; Yao, Zhikan; Wang, Jing; Zhang, Lin; Tang, Zhen

doi:10.1016/j.memsci.2022.121134

Cited by 14 publications

(9 citation statements)

References 56 publications

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“…However, due to limitations in experimental methods, obtaining information about the orientation, conformation and interaction of biological molecules such as proteins during their adsorption process is challenging [11,12]. It is not possible to visually show the adsorption process of proteins at the molecular level [13][14][15]. This paper utilizes Molecular Dynamics (MD) method to analyze protein adsorption on membrane surfaces from a molecular perspective [16,17].…”

Section: Introductionmentioning

confidence: 99%

Simulation of interaction between modified polysulfone membrane surface and blood protein interface

Yan,

Qiu

2024

Preprint

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In this study, the adsorption mechanism of protein on functionalized modified polysulfone membrane was investigated by molecular dynamics method (MD). The interaction between different functionalized membrane surfaces and protein adsorption was revealed. The modified polysulfone membranes with different end-group chemical functional groups were prepared by chemical synthesis method. The orientation and spatial conformation changes of protein adsorption at molecular level were investigated by theoretical analysis, basic experiment and MD simulation. The results showed that BSA exhibited different variability and adsorption properties on the membrane modified with different functional groups. On the positively charged surface, BSA exhibits the most unstable configurational stability and is prone to non-specific structural changes. In addition, the surface charge effect leads to electrostatic repulsion between BSA, reducing the number of protein adsorption sites, which is consistent with the simulation and experimental characterization results. These MD simulation results are consistent with the experimental results, and provide a new design idea and support for the modification of blood compatible membrane materials.

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Section: Introductionmentioning

confidence: 99%

Simulation of interaction between modified polysulfone membrane surface and blood protein interface

Yan,

Qiu

2024

Preprint

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“…However, due to limitations in experimental methods, obtaining information about the orientations, conformations, and interactions of biological molecules such as proteins during their adsorption process is challenging [ 9 ]. It is impossible to intuitively demonstrate the process of protein adsorption at a molecular level [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Interfacial Interaction between Functionalization of Polysulfone Membrane Materials and Protein Adsorption

Yan,

Qiu

2024

Polymers

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This study that modified polysulfone membranes with different end-group chemical functionalities were prepared using chemical synthesis methods and experimentally characterized. The molecular dynamics (MD) method were used to discuss the adsorption mechanism of proteins on functionalized modified polysulfone membrane materials from a molecular perspective, revealing the interactions between different functionalized membrane surfaces and protein adsorption. Theoretical analysis combined with basic experiments and MD simulations were used to explore the orientation and spatial conformational changes of protein adsorption at the molecular level. The results show that BSA exhibits different variability and adsorption characteristics on membranes with different functional group modifications. On hydrophobic membrane surfaces, BSA shows the least stable configuration stability, making it prone to nonspecific structural changes. In addition, surface charge effects lead to electrostatic repulsion for BSA and reduce the protein adsorption sites. These MD simulation results are consistent with experimental findings, providing new design ideas and support for modifying blood-compatible membrane materials.

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“…5 Endotoxin is a known causative factor in the occurrence and progression of sepsis, and blood purification is one of the main techniques to reduce endotoxin levels and improve the survival rate of patients with sepsis. 6 So far, several extracorporeal blood purification materials, such as polymyxin B-immobilized polystyrene fiber column, 7 polyethylenimine (PEI) coating, and heparin pre-grafted AN69 membrane, 8 charge-biased PSF electrospinning nanofiber membrane, 9 and chitosan hydrogel 6 have been used in removing endotoxins from blood. However, these materials also have problems such as low adsorption efficiency, small adsorption capacity, no antibacterial properties, and poor blood compatibility.…”

Section: Introductionmentioning

confidence: 99%

“…The ways in which people try to modify hemodialysis membranes mainly include blending [19][20][21] and surface coating, 22 surface chemical modification, [23][24][25] and mixed charge modification. 9,[26][27][28] In mixed charge modification strategies, charged polymers play an important role. PEI is a traditional cationic polymer with antibacterial activity.…”

Section: Introductionmentioning

confidence: 99%

Mixed‐charge cellulose nanocrystal modified cellulose acetate membrane with endotoxin scavenging ability and antibacterial properties

Zhou,

Zhang,

Xia

et al. 2024

J of Applied Polymer Sci

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Blending modification is used to improve the hemocompatibility of cellulose acetate (CA) dialysis membranes while endowing the CA membrane with endotoxin scavenging and antibacterial capabilities. First, polydopamine (PDA) and polyethylenimine (PEI) are used as negatively charged and positively charged moieties, respectively, together with cellulose nanocrystals (CNCs) to construct mixed charge CNCs (PEI‐PDA@CNCs). Then PEI‐PDA@CNCs is blended with the CA casting solution, and the modified CA dialysis membrane is prepared through a phase inversion process. Through biocompatibility, adsorption, antibacterial, and filtration experiments, various properties of PEI‐PDA@CNCs modified CA membrane (PDCA) are systematically tested. The results show that the PDCA membrane exhibited good blood compatibility and noncytotoxicity. At the same time, the PDCA membrane has antibacterial properties and excellent endotoxin adsorption capacity. Compared with the CA membrane, the clearance rates of PDCA membrane for Staphylococcus aureus and Escherichia coli reach 54.0% ± 1.5% and 52.9% ± 5.9% respectively. In the dynamic adsorption experiment, the adsorption capacity of PDCA membrane for endotoxin increases to 2322.1 ± 45.9 EU/g. The research results indicate that PEI‐PDA@CNC‐modified multifunctional CA membrane with endotoxin adsorption and antibacterial properties has potential application prospects in the fields of hemodialysis and blood purification.

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Charge-biased nanofibrous membranes with uniform charge distribution and hemocompatibility for enhanced selective adsorption of endotoxin from plasma

Cited by 14 publications

References 56 publications

Simulation of interaction between modified polysulfone membrane surface and blood protein interface

Simulation of interaction between modified polysulfone membrane surface and blood protein interface

Interfacial Interaction between Functionalization of Polysulfone Membrane Materials and Protein Adsorption

Mixed‐charge cellulose nanocrystal modified cellulose acetate membrane with endotoxin scavenging ability and antibacterial properties

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