Charge distribution in the surface region of human erythrocytes as estimated from electrophoretic mobility data

Electrophoretic Mobility of Mouse Leukocytes Evaluated Using Microcapillary Electrophoresis Chips

“…Bubenikowa et al reported that EPM of mouse primary myeloma cells is −1.2×10 −4 cm 2 V -1 s -1 (ionic strength 0.14) [25]. Cell EPM is hardly affected by ionic strength of buffer solution when the ionic strength is over 0.05 [26,27]. Hence, EPMs obtained in this study are comparable and agree relatively well with previously reported data.…”

The effect of motility and cell-surface polymers on bacterial attachment

“…However, the observations for various kinds of biological cells (van Loosdrecht et al, 1987 ;Kawahata et al, 1990 ;Rijnaarts et al, 1995 ;Sonohara et al, 1995 ;Takashima & Morisaki, 1997) hardly obey this prediction. Although it has been pointed out that surface conductance on bacterial cells may have a considerable effect on the electrophoretic mobility of the cells at a low ionic strength (van der Wal et al, 1997), the changing pattern of EPM as shown in Fig.…”

The electrophoretic softness of the surface of Staphylococcus epidermidis cells grown in a liquid medium and on a solid agar

“…The polyelectrolyte layer around microbial cell surfaces allows electrophoretic fluid flow, and the ease by which fluid can flow through the soft layer is expressed as a so-called electrophoretic softness, 1\λ. The theory of Ohshima enables quantitative determination of the volumetric charge density and the softness (1\λ) of this polyelectrolyte layer from the dependence of the electrophoretic mobility upon ionic strength, and it has been successfully applied to describe the surfaces of human erythrocytes (Kawahata et al, 1990) and rat lymphocytes (Morita et al, 1991). Recently, we studied the softness of two oral streptococcal strains, Streptococcus salivarius HB and HBC12, by particulate microelectrophoresis in KCl solutions of varying ionic strengths (Bos et al, 1998).…”