Charge location on gas phase peptides

Hill, Herbert H.; Hill, Chandler; Asbury, G. Reid; Wu, Ching; Matz, Laura M.; Ichiye, Toshiko

doi:10.1016/s1387-3806(02)00557-2

Cited by 49 publications

(59 citation statements)

References 33 publications

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“…An RIP is commonly observed with ionisation sources such as 63 Ni and corona discharge, and some researchers have argued that using the RIP is the most convenient and efficient way of calibrating their IMS systems. Such an approach was exemplified by Tabrizchi [11] who proposed using [(H 2 O) 2 H] + , as an internal reference standard.…”

Section: Current Status Of Standardisation In Imsmentioning

confidence: 99%

Chemical standards for ion mobility spectrometry: a review

Kaur-Atwal

O’Connor

Aksenov

et al. 2009

Int. J. Ion Mobil. Spec.

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Ion mobility spectrometry (IMS), using standalone instrumentation and hyphenated with mass spectrometry (IM-MS), has recently undergone significant expansion in the numbers of users and applications, particularly in sectors outside its established user base; predominantly military and security applications. Although several IMS reference standards have been proposed, there are no currently universally recognised reference standards for the calibration and evaluation of mobility spectrometers. This review describes current practices and the literature on chemical standards for validating IMS systems in positive and negative ion modes. The key qualities and requirements an 'ideal' reference standard must possess are defined, together with the instrumental and environmental factors such as temperature, electric field, humidity and drift gas composition that may need to be considered. Important challenges that have yet to be resolved are also identified and proposals for future development presented.

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Section: Current Status Of Standardisation In Imsmentioning

confidence: 99%

Chemical standards for ion mobility spectrometry: a review

Kaur-Atwal

O’Connor

Aksenov

et al. 2009

Int. J. Ion Mobil. Spec.

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“…Ionized sample molecules were desolvated prior to ion gating using a counter-current flow (ϳ1 L/min) of heated nitrogen drift gas held at 200°C. With a larger center of mass and collision cross section, nitrogen allows for longer ion drift times, and consequently higher resolving powers, when compared to drift gases such as helium [27,33]. The ambient pressure in Pullman, WA was approximately 700 torr.…”

Section: Electrospray Ionization-atmospheric Pressure Ion Mobility-timentioning

confidence: 99%

“…Where z is the number of the charges on the ion; e the charge of one proton; N the number density of the drift gas at standard conditions, ϭ [mM/(m ϩ M)] the reduced mass of an ion (m) and the neutral drift gas (M), and k, Boltzmann's constant [33,37]. Using a variety of computational methods it is possible to gain insight into the structure of gas-phase ions by correlating experimentally determined collision cross sections with theoretically derived structures [29, 33, 34, 38 -40].…”

Section: Reduced Mobility Constants Resolving Power and Experimentamentioning

confidence: 99%

Separation of sodiated isobaric disaccharides and trisaccharides using electrospray ionization-atmospheric pressure ion mobility-time of flight mass spectrometry

Clowers

Dwivedi

Steiner

et al. 2005

J. Am. Soc. Mass Spectrom.

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158

167

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A series of isobaric disaccharide-alditols, four derived from O-linked glycoproteins, and select trisaccharides were rapidly resolved using tandem high resolution atmospheric pressure ion-mobility time-of-flight mass spectrometry. Electrospray ionization was used to create the gas-phase sodium adducts of each carbohydrate. Using this technique it was possible to separate up to three isobaric disaccharide alditols and three trisaccharides in the gas phase.Reduced mobility values and experimentally determined ion-neutral cross sections are reported for each sodium-carbohydrate complex. These studies demonstrated that ion mobility separations at atmospheric pressure can provide a high-resolution dimension for analysis of carbohydrate ions that is complementary to traditional mass spectral (m/z) ion analysis. Combining these independent principles for separation of ions provides a powerful new bioanalytical tool for the identification of isomeric carbohydrates. In order to fully characterize oligosaccharides derived from biological sources, a number distinct of challenges must be over come. These issues arise primarily from the existence of biological oligosaccharides as sets of isomers. A number of approaches to address isomeric carbohydrate structures using mass spectrometry have been reported. These methods include periodate oxidation/borohydride reduction, followed by hydroxyl methylation or peracetylation [9, 10], derivatization of monosaccharides or short oligosaccharides with amines such as diethylenetriamine at the reducing end followed by metal complexation [11,12], or prediction of possible fragmentation pathways after permethylation [13]. Although modern mass spectrometry is an exquisite tool in itself for the separation of molecules having different m/z values, it cannot rule out the possibility that mass spectra derived from selected precursor ions are not derived from an isobaric mixture. And to complicate matters even further, it is entirely possible that the fragment ions themselves are isobars. On a fundamental level, the stereochemistry of monosaccharides, as product ions derived from a larger molecule, cannot be unambiguously established from a fragmentation pattern. Aldohexoses, for example, come in sixteen different stereochemical variants, and fragmentation data that would uniquely differentiate each of them has yet to be convincingly furnished, particularly where they are derived as product ions from larger molecules. To address the stereochemical blindness of mass spectrometry, product ions must first be separated based upon a physical principle that is not dependent upon m/z prior to fragmentation.Traditional chromatographic methods, most notably liquid and gas, have been used to provide an additional

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“…Only few investigations were published on other applications such as biological samples [15][16][17][18]. At ISAS-Institute for Analytical Sciences, Dortmund, Germany, ion mobility spectrometry with fast pre-separation techniques was applied for the sensitive detection (lower ppb v down to ppt v range) of metabolites in human breath [19,20] but also for process control and food quality and safety [21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Exemplar application of multi-capillary column ion mobility spectrometry for biological and medical purpose

Vautz

Baumbach

2008

Int. J. Ion Mobil. Spec.

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In recent years, ion mobility spectrometry is increasingly in demand for new applications especially on biological samples (cells, bacteria, fungi), in medicine (diagnosis, therapy and medication control e.g. from breath analyses), for food quality control, safety monitoring and characterisation or process control in chemical and pharmaceutical industry. For this purpose instruments based on gas phase separation of ions in weak electric fields were developed at ISAS-Institute for Analytical Sciences, focussing on the particular challenges such as humid and rather complex samples, specific sampling procedures adapted to the application, fast pre-separation techniques like multi-capillary columns and suitable data processing including data bases for relevant analytes and automatic characterisation of IMS-chromatograms. Feasibility studies were carried out successfully for biological and medical purpose at ISAS, including the detection of bacteria, fungi and metabolites of cells and in human breath. For all those samples characteristic pattern of analytes were found and could be used for the identification of cell lines, fungi and bacteria as well as of numerous diseases. Furthermore, the quantification of those analytes could be used to obtain information about the state of the process or person (e.g. growth of cultures, development of diseases, level of medication, grade of cancer). Those examples shall demonstrate the potential of ion mobility spectrometry for the selected applications. However, a general and reliable data bases of reference analytes is required in the near future to enable an exploitation of the metabolic pathways and to confirm the relevance of the detected signals for the investigated topic.

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Charge location on gas phase peptides

Cited by 49 publications

References 33 publications

Chemical standards for ion mobility spectrometry: a review

Chemical standards for ion mobility spectrometry: a review

Separation of sodiated isobaric disaccharides and trisaccharides using electrospray ionization-atmospheric pressure ion mobility-time of flight mass spectrometry

Exemplar application of multi-capillary column ion mobility spectrometry for biological and medical purpose

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