The longitudinal ultrasonic velocity (V ), as well as resistivity and magnetization has been measured in polycrystalline R 1/3 Sr 2/3 FeO3 (R = La, Pr, Gd). When the R-site ion is changed from La to smaller-size R ion, the charge ordering (CO) transition shifts to lower temperature and finally disappears for R = Gd. Dramatic anomalies in V for the R = La, Pr compounds are observed near the CO transition temperature, which implies the electron-phonon coupling arising from the Jahn-Teller effect of Fe 4+ . And the theory calculation result shows that this coupling is weakened for Pr, which may lead to the shift of TCO. In the case of Gd 1/3 Sr 2/3 FeO3 with no CO transition, the anomaly in V is small, and may be attributed to the antiferromagnetic spin fluctuations. Below TCO, another unexpected softening in V is discerned in La 1/3 Sr 2/3 FeO3, while only a slope change is observed in Pr 1/3 Sr 2/3 FeO3. This feature is attributed to the breathing-type distortion of Fe-O octahedron via the charge disproportionation, which is weakened in Pr 1/3 Sr 2/3 FeO3.