Charge reversal by salt-induced aggregation in aqueous lactoferrin solutions

Mela, Ioanna; Aumaitre, Elodie; Williamson, Ann-Marie; Yakubov, Gleb E.

doi:10.1016/j.colsurfb.2010.02.011

Cited by 30 publications

(29 citation statements)

References 28 publications

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“…In the case of LF, the effect of the electrolyte concentration on the zeta potential was more significant, with values isoelectric point ranging from 9.3 (0.01 M) to 5.7 (0.1 M). This is consistent with the large range of isoelectric points that have been reported so far for this protein (8.0 9.0 for 0.01 M NaCl [24]; 7.2 for 0.025 M KCl [25]; 5.6 for 0.15 M NaCl [12]). It is well known that when the electrolyte concentration is increased, the surface charge is compensated at a lower distance from the particle surface and thus, the surface potential drops faster and the diffuse layer is thinner.…”

Section: Zeta Potential Measurementssupporting

confidence: 76%

Accurate determination of key surface properties that determine the efficient separation of bovine milk BSA and LF proteins

Valiño

Román

Ibáñez

et al. 2014

Separation and Purification Technology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. KeywordsDynamic light scattering, Zeta Potential, Bovine Serum Proteins, Bioprocess Design. AbstractThe aim of this work is to accurately measure fundamental surface properties, i.e., zeta potential, isoelectric point and protein size that determine the optimal separation conditions of Bovine serum albumin and lactoferrin, two high added value food proteins whose similarity in weight makes their separation a scientific and technical challenge.

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Section: Zeta Potential Measurementssupporting

confidence: 76%

Accurate determination of key surface properties that determine the efficient separation of bovine milk BSA and LF proteins

Valiño

Román

Ibáñez

et al. 2014

Separation and Purification Technology

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“…Thus, we have investigated the adsorption of one layer of BSA, the most abundant plasma protein in mammals, and to lactoferrin (Lf) onto LysMBs surface. Lf is an iron-glycoprotein belonging to the innate immune system which plays a relevant role in the iron metabolism (Bellamy et al 1993;Muller et al 1999;Wakabayashi et al 2002;Superti et al 1997;Swart et al 1998;Semba et al 1998;Ikeda et al 2000;Inoue et al 2001), and has several anti-inflammatory properties (Elrod et al 1997); in fact, the human Lf has also been shown to be protective in inflammation induced by allergic asthma (Mela et al 2010).…”

Section: Interaction Of the Lysmbs With Other Proteinsmentioning

confidence: 98%

Molecular properties of lysozyme-microbubbles: towards the protein and nucleic acid delivery

et al. 2011

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Microbubbles (MBs) have specific acoustic properties that make them useful as contrast agents in ultrasound imaging. The use of the MBs in clinical practice led to the development of more sensitive imaging techniques both in cardiology and radiology. Protein-MBs are typically obtained by dispersing a gas phase in the protein solution and the protein deposited/cross-linked on the gas-liquid interface stabilizes the gas core. Innovative applications of protein-MBs prompt the investigation on the properties of MBs obtained using different proteins that are able to confer them specific properties and functionality. Recently, we have synthesized stable air-filled lysozyme-MBs (LysMBs) using high-intensity ultrasound-induced emulsification of a partly reduced lysozyme in aqueous solutions. The stability of LysMBs suspension allows for post-synthetic modification of MBs surface. In the present work, the protein folded state and the biodegradability property of LysMBs were investigated by limited proteolysis. Moreover, LysMBs were coated and functionalized with a number of biomacromolecules (proteins, polysaccharides, nucleic acids). Remarkably, LysMBs show a high DNA-binding ability and protective effects of the nucleic acids from nucleases and, further, the ability to transform the bacteria cells. These results highlight on the possibility of using LysMBs for delivery of proteins and nucleic acids in prophylactic and therapeutic applications.

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“…LF exhibits ionic strength-dependent self-assembly behavior [44][45][46]. At neutral pH and low ionic strength, LF is positively charged and mainly monomer but forms neutral [44] or negatively charged assemblies (pI6) [45] when ionic strength increased.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Heteroprotein complex coacervation: A generic process

Croguennec

Tavares

Bouhallab

2017

Advances in Colloid and Interface Science

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Proteins exhibit a rich diversity of functional, physico-chemical and biodegradable properties which makes them appealing for various applications in the food and non-food sectors. Such properties are attributed to their ability to interact and assemble into a diversity of supramolecular structures. The present review addresses the updated research progress in the recent field of complex coacervation made from mixtures of oppositely charged proteins (i.e. heteroprotein systems). First, we describe briefly the main proteins used for heteroprotein coacervation. Then, through some selected examples, we illustrate the particularity and specificity of each heteroprotein system and the requirements that drive optimal assembly into coacervates. Finally, possible and promising applications of heteroprotein coacervates are mentioned.

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Charge reversal by salt-induced aggregation in aqueous lactoferrin solutions

Cited by 30 publications

References 28 publications

Accurate determination of key surface properties that determine the efficient separation of bovine milk BSA and LF proteins

Accurate determination of key surface properties that determine the efficient separation of bovine milk BSA and LF proteins

Molecular properties of lysozyme-microbubbles: towards the protein and nucleic acid delivery

Heteroprotein complex coacervation: A generic process

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