Charge reversible calcium phosphate lipid hybrid nanoparticle for siRNA delivery

Cai, Rong-Qiao; Liu, Dao-Zhou; Han, Chunchun; Cheng, Ying; Liu, Miao; Zhang, Bang-Le; Mei, Qibing; Zhou, Siyuan

doi:10.18632/oncotarget.17484

Cited by 16 publications

(8 citation statements)

References 43 publications

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“…To make the system pH-triggered charge-reversible, citraconic as a derivative of a maleic amide has been conjugated with cholesterol-aminocaproic acid to coat calcium phosphate particles and the further surface has been tuned by depositing the siRNA. In the last, the positively charged lipid 1,2-dioleoyl-3-trimethylammonium-propanechloridesalt (DOTAP) and negatively charged lipid dioleoylphosphatydicacid (DOPA) has been added and the charge reversal was investigated with the reversal of zeta potential results, which successfully suppress the Bcl-2 without inducing any toxicity in lung cancer A549 cells [ 50 ]. In some cases, the carrier should be negatively charged to achieve efficient transfection whilst positively charged in an acidic endolysosome environment to inhibit its uptake by disrupting the endolysosomal membrane.…”

Section: Strategies In Bone Targetingmentioning

confidence: 99%

“…Moving ahead, the research has been proposed by Cai et al [ 50 ], wherein calcium phosphate–lipid hybrid nanoparticles are associated with the charge reversal property were synthesized for gene silencing purposes in lung cancer. SiRNA Bcl-2 gene via phosphate–lipid hybrid nanoparticles successfully reached the cytoplasm and expression of Bcl-2 in A549 cells.…”

Section: Strategies In Bone Targetingmentioning

confidence: 99%

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Lipid-Based Nano-Sized Cargos as a Promising Strategy in Bone Complications: A Review

et al. 2022

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Bone metastasis has been considered the fatal phase of cancers, which remains incurable and to be a challenge due to the non-availability of the ideal treatment strategy. Unlike bone cancer, bone metastasis involves the spreading of the tumor cells to the bones from different origins. Bone metastasis generally originates from breast and prostate cancers. The possibility of bone metastasis is highly attributable to its physiological milieu susceptible to tumor growth. The treatment of bone-related diseases has multiple complications, including bone breakage, reduced quality of life, spinal cord or nerve compression, and pain. However, anticancer active agents have failed to maintain desired therapeutic concentrations at the target site; hence, uptake of the drug takes place at a non-target site responsible for the toxicity at the cellular level. Interestingly, lipid-based drug delivery systems have become the center of interest for researchers, thanks to their biocompatible and bio-mimetic nature. These systems possess a great potential to improve precise bone targeting without affecting healthy tissues. The lipid nano-sized systems are not only limited to delivering active agents but also genes/peptide sequences/siRNA, bisphosphonates, etc. Additionally, lipid coating of inorganic nanomaterials such as calcium phosphate is an effective approach against uncontrollable rapid precipitation resulting in reduced colloidal stability and dispersity. This review summarizes the numerous aspects, including development, design, possible applications, challenges, and future perspective of lipid nano-transporters, namely liposomes, exosomes, solid lipid nanoparticles (SLN), nanostructured lipid carriers (NLC), and lipid nanoparticulate gels to treat bone metastasis and induce bone regeneration. Additionally, the economic suitability of these systems has been discussed and different alternatives have been discussed. All in all, through this review we will try to understand how far nanomedicine is from clinical and industrial applications in bone metastasis.

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Section: Strategies In Bone Targetingmentioning

confidence: 99%

Section: Strategies In Bone Targetingmentioning

confidence: 99%

Lipid-Based Nano-Sized Cargos as a Promising Strategy in Bone Complications: A Review

et al. 2022

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“…The impact of the nanomaterials' surface charge on their ability to penetrate spheroids has also been a studied subject [43,[76][77][78][79][80][81]. In this regard, Jiang group demonstrated that negatively charged polystyrene-based nanoparticles (ζ potential of -34.9 mV) have a better ability to penetrate HepG2 spheroids when compared with their positively charged equivalents (+9.2 mV) [43].…”

Section: Nanomedicines' Chargementioning

confidence: 99%

The Importance of Spheroids in Analyzing Nanomedicine Efficacy

Mó

Sabino

Melo‐Diogo

et al. 2020

Nanomedicine (Lond.)

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The use of nanomedicines for cancer treatment holds a great potential due to their improved efficacy and safety. During the nanomedicine preclinical in vitro evaluation stage, these are mainly tested on cell culture monolayers. However, these 2D models are an unrealistic representation of the in vivo tumors, leading to an inaccurate screening of the candidate formulations. To address this problem, spheroids are emerging as an additional tool to validate the efficacy of new therapeutics due to the ability of these 3D in vitro cancer models to mimic the key features displayed by in vivo solid tumors. In this review, the application of spheroids for the evaluation of nanomedicines’ physicochemical properties and therapeutic efficacy is discussed.

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“…The haemolysis effect of DSPE-DEX and DSPE-DEX-ALN was evaluated by using UV-Vis spectrophotometer [21].…”

Section: Cytotoxicity Of Dox@dbms-alnmentioning

confidence: 99%

Bone metastasis target redox-responsive micell for the treatment of lung cancer bone metastasis and anti-bone resorption

Zhao

Cheng

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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In order to inhibit the growth of lung cancer bone metastasis and reduce the bone resorption at bone metastasis sites, a bone metastasis target micelle DOX@DBMs-ALN was prepared. The size and the zeta potential of DOX@DBNs-ALN were about 60 nm and -15 mV, respectively. DOX@DBMs-ALN exhibited high binding affinity with hydroxyapatite and released DOX in redox-responsive manner. DOX@DBMs-ALN was effectively up taken by A549 cells and delivered DOX to the nucleus of A549 cells, which resulted in strong cytotoxicity on A549 cells. The in vivo experimental results indicated that DOX@DBMs-ALN specifically delivered DOX to bone metastasis site and obviously prolonged the retention time of DOX in bone metastasis site. Moreover, DOX@DBMs-ALN not only significantly inhibited the growth of bone metastasis tumour but also obviously reduced the bone resorption at bone metastasis sites without causing marked systemic toxicity. Thus, DOX@DBMs-ALN has great potential in the treatment of lung cancer bone metastasis.

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Charge reversible calcium phosphate lipid hybrid nanoparticle for siRNA delivery

Cited by 16 publications

References 43 publications

Lipid-Based Nano-Sized Cargos as a Promising Strategy in Bone Complications: A Review

Lipid-Based Nano-Sized Cargos as a Promising Strategy in Bone Complications: A Review

The Importance of Spheroids in Analyzing Nanomedicine Efficacy

Bone metastasis target redox-responsive micell for the treatment of lung cancer bone metastasis and anti-bone resorption

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