Charge transport properties of SARS-CoV-2 Delta variant (B.1.617.2)

He, L. L.; Xie, Zhiyang; Long, Xing; Zhang, Chaopeng; He, Chengyun; Zhao, Boyang; Qi, Fei; Zhang, Nan

doi:10.1016/j.procbio.2022.08.010

Cited by 4 publications

(3 citation statements)

References 23 publications

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“…These results show that the screening method used in this study can distinguish between variants of SARS-CoV-2, which is essential for early screening and diagnosis. Our findings are consistent with recent theoretical approaches that propose COVID detection using conductance variation caused by single nucleotide differences between SARS-CoV-2 and its five variants of concern (Alpha, Beta, Gamma, Delta and Omicron) (He et al, 2022(He et al, , 2023.…”

Section: Bioinformatics Analysis and Dna Probe Designsupporting

confidence: 92%

“…(Arachchillage et al, 2023;Chandra et al, 2022;Li et al, 2016aLi et al, ,b, 2018) Also, recent theoretical approaches propose that the molecular conductance of nucleic acids from different SARS-CoV-2 variants should be in the 10 −7 G 0 -10 −4 G 0 range, in agreement with our findings. (He et al, 2022(He et al, , 2023 Small faradaic (non-tunneling) currents and the noise of the current amplifier result in a tip lekeage feature in the histogram, representing the lower conductance range typical in these measurements in buffer. (Artés et al, 2015)…”

Section: Proof Of Concept Covid-19 Single Molecule Electrical Biosensormentioning

confidence: 97%

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A single-molecule RNA electrical biosensor for COVID-19

Arachchillage

Chandra

Williams

et al. 2023

Preprint

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The COVID-19 pandemic shows a critical need for rapid, inexpensive, and ultrasensitive early detection methods based on biomarker analysis to reduce mortality rates by containing the spread of epidemics. This can be achieved through electrical detection of nucleic acids at the single-molecule level. In particular, the scanning tunneling microscopic-assisted break junction (STM-BJ) method can be utilized to detect individual nucleic acid molecules with high specificity and sensitivity in liquid samples. Herein, we demonstrate single-molecule electrical detection of RNA coronavirus biomarkers, including those of SARS-CoV-2 as well as those of different variants and subvariants. Our target sequences include a conserved sequence in the human coronavirus family, a conserved target specific for the SARS-CoV-2 family, and specific targets at the variant and subvariant levels. Our results demonstrate that it is possible to distinguish between different variants of the COVID-19 virus using electrical conductance signals, as recently suggested by theoretical approaches. Furthermore, we propose a strategy to detect new variants by analyzing electrical fingerprints from multiple sequences. This could allow for a rapid response early in new outbreaks. These results pave the way for future miniaturized single-molecule electrical biosensors that could be game changers for the COVID-19 pandemic, other infectious diseases, and several other public health applications.

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Section: Bioinformatics Analysis and Dna Probe Designsupporting

confidence: 92%

Section: Proof Of Concept Covid-19 Single Molecule Electrical Biosensormentioning

confidence: 97%

A single-molecule RNA electrical biosensor for COVID-19

Arachchillage

Chandra

Williams

et al. 2023

Preprint

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“…This capability is essential for early diagnosis and screening. These findings align with recent theoretical approaches that suggest using variations in conductance resulting from single nucleotide differences to detect COVID-19 and its variants of concern (Alpha, Beta, Gamma, Delta, and Omicron). , These results also pave the way for future possibilities such as highly automatized and miniaturized electrical biosensors to monitor these RNA sequences, if obvious roadblocks in miniaturization and microfabrication of nanoelectrodes can be solved. In a scenario where these sensors can be miniaturized and parallelized into several single-molecule electrical detection channels, future strategies can be proposed for the electrical fingerprinting of COVID-19 samples (Figure (d)).…”

Section: A Brief Introduction To Biosensorssupporting

confidence: 86%

Biosensors for Public Health and Environmental Monitoring: The Case for Sustainable Biosensing

Williams,

Aguilar,

Pattiya Arachchillage

et al. 2024

ACS Sustainable Chem. Eng.

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Climate change is a profound crisis that affects every aspect of life, including public health. Changes in environmental conditions can promote the spread of pathogens and the development of new mutants and strains. Early detection is essential in managing and controlling this spread and improving overall health outcomes. This perspective article introduces basic biosensing concepts and various biosensors, including electrochemical, optical, mass-based, nano biosensors, and single-molecule biosensors, as important sustainability and public health preventive tools. The discussion also includes how the sustainability of a biosensor is crucial to minimizing environmental impacts and ensuring the long-term availability of vital technologies and resources for healthcare, environmental monitoring, and beyond. One promising avenue for pathogen screening could be the electrical detection of biomolecules at the single-molecule level, and some recent developments based on single-molecule bioelectronics using the Scanning Tunneling Microscopy-assisted break junctions (STM-BJ) technique are shown here. Using this technique, biomolecules can be detected with high sensitivity, eliminating the need for amplification and cell culture steps, thereby enhancing speed and efficiency. Furthermore, the STM-BJ technique demonstrates exceptional specificity, accurately detects single-base mismatches, and exhibits a detection limit essentially at the level of individual biomolecules. Finally, a case is made here for sustainable biosensors, how they can help, the paradigm shift needed to achieve them, and some potential applications.

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