Charged hybrid block copolymer-lipid-cholesterol vesicles: pH, ionic environment, and composition dependence of phase transitions

Hamada, Naomi; Longo, Marjorie L.

doi:10.1016/j.bbamem.2022.184026

Cited by 2 publications

(1 citation statement)

References 27 publications

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“…This approach reveiled that alterations in both pH and salt concentration induced a shift in the transitional phase of sHVs composed of carboxylated PEG- b -PBd & egg sphingomyelin (egg-SM), while variations in molecular weight had no effect. 65 The application of multipeak analysis to gHVs consisting of the same components (PEG- b -PBd & egg-SM and cholesterol) provided insights into the mechanism governing phase separation during the cooling of gHVs. 63 The analysis suggested either spinodal decomposition or nucleation and growth as the underlying processes.…”

Section: Membrane Propertiesmentioning

confidence: 99%

Advances in block copolymer-phospholipid hybrid vesicles: from physical–chemical properties to applications

Brodszkij,

Städler

2024

Chem. Sci.

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Section: Membrane Propertiesmentioning

confidence: 99%

Advances in block copolymer-phospholipid hybrid vesicles: from physical–chemical properties to applications

Brodszkij,

Städler

2024

Chem. Sci.

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Poly(Sitosterol)‐Based Hydrophobic Blocks in Amphiphilic Block Copolymers for the Assembly of Hybrid Vesicles

Brodszkij,

Ryberg,

Lyons

et al. 2024

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Amphiphilic block copolymer and lipids can be assembled into hybrid vesicles (HVs), which are an alternative to liposomes and polymersomes. Block copolymers that have either poly(sitostryl methacrylate) or statistical copolymers of sitosteryl methacrylate and butyl methacrylate as the hydrophobic part and a poly(carboxyethyl acrylate) hydrophilic segment are synthesized and characterized. These block copolymers assemble into small HVs with soybean L‐α‐phosphatidylcholine (soyPC), confirmed by electron microscopy and small‐angle X‐ray scattering. The membrane's hybrid nature is illustrated by fluorescence resonance energy transfer between labeled building blocks. The membrane packing, derived from spectra when using Laurdan as an environmentally sensitive fluorescent probe, is comparable between small HVs and the corresponding liposomes with molecular sitosterol, although the former show indications of transmembrane asymmetry. Giant HVs with homogenous distribution of the block copolymers and soyPC in their membranes are assembled using the electroformation method. The lateral diffusion of both building blocks is slowed down in giant HVs with higher block copolymer content, but their permeability toward (6)‐carboxy‐X‐rhodamine is higher compared to giant vesicles made of soyPC and molecular sitosterol. This fundamental effort contributes to the rapidly expanding understanding of the integration of natural membrane constituents with designed synthetic compounds to form hybrid membranes.

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Charged hybrid block copolymer-lipid-cholesterol vesicles: pH, ionic environment, and composition dependence of phase transitions

Cited by 2 publications

References 27 publications

Advances in block copolymer-phospholipid hybrid vesicles: from physical–chemical properties to applications

Advances in block copolymer-phospholipid hybrid vesicles: from physical–chemical properties to applications

Poly(Sitosterol)‐Based Hydrophobic Blocks in Amphiphilic Block Copolymers for the Assembly of Hybrid Vesicles

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