Charged MVB protein 5 is involved in T-cell receptor signaling

Wi, Sae Mi; Min, Young Ki; Lee, Ki-Young

doi:10.1038/emm.2015.102

Cited by 5 publications

(4 citation statements)

References 31 publications

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“…Additionally, CHMP5 prevents Bcl-2, a widely recognized apoptosis suppressor gene that intrinsically regulates apoptosis, from deleterious oxidation by reactive oxygen species (ROS) formation ( Adoro et al, 2017 ). Furthermore, it has been shown that CHMP5 has a key role in T-cell receptor signaling and its deficiency affects T-cell receptor expression on the cell surface ( Wi et al, 2016 ). However, the role of CHMP5 in IS has not been studied to date.…”

Section: Discussionmentioning

confidence: 99%

Expression pattern and clinical value of Key RNA methylation modification regulators in ischemic stroke

et al. 2022

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Ischemic stroke (IS) is one of the major causes of death and disability worldwide, and effective diagnosis and treatment methods are lacking. RNA methylation, a common epigenetic modification, plays an important role in disease progression. However, little is known about the role of RNA methylation modification in the regulation of IS. The aim of this study was to investigate RNA methylation modification patterns and immune infiltration characteristics in IS through bioinformatics analysis. We downloaded gene expression profiles of control and IS model rat brain tissues from the Gene Expression Omnibus database. IS profiles were divided into two subtypes based on RNA methylation regulators, and functional enrichment analyses were conducted to determine the differentially expressed genes (DEGs) between the subtypes. Weighted gene co-expression network analysis was used to explore co-expression modules and genes based on DEGs. The IS clinical diagnosis model was successfully constructed and four IS characteristic genes (GFAP, GPNMB, FKBP9, and CHMP5) were identified, which were significantly upregulated in IS samples. Characteristic genes were verified by receiver operating characteristic curve and real-time quantitative PCR analyses. The correlation between characteristic genes and infiltrating immune cells was determined by correlation analysis. Furthermore, GPNMB was screened using the protein-protein interaction network, and its regulatory network and the potential therapeutic drug chloroquine were predicted. Our finding describes the expression pattern and clinical value of key RNA methylation modification regulators in IS and novel diagnostic and therapeutic targets of IS from a new perspective.

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Section: Discussionmentioning

confidence: 99%

Expression pattern and clinical value of Key RNA methylation modification regulators in ischemic stroke

et al. 2022

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“…To generate control (Ctrl) THP-1 and AMPKα1KD THP-1 cells, lentivirus containing small hairpin RNA (shRNA) targeting human AMPKαl (Santa Cruz Biotechnology, sc-29673-V, Santa Cruz, CA, USA) and control shRNA lentivirus (Santa Cruz Biotechnology, Santa Cruz, sc-108080, Santa Cruz, CA, USA) were used to infect THP-1 cells. Ctrl THP-1 and AMPKα1KD THP-1 cells were selected as previously described [8,[23][24][25][26].…”

Section: Generation Of Ampkα1-knockdown (Ampkα1kd) Thp-1 Cellsmentioning

confidence: 99%

“…Western blotting and IP assays were performed as previously described [13,15,16,[23][24][25][26][27][28][29][30][31]. Briefly, mock vector as control vector, MYC-TRAF6, FLAG-AMPKαl, and MYC-BECN1 were transfected into HEK293T cells using Lipofectamine 2000 for 38-48 h. Cell lysates were immunoprecipitated with anti-FLAG or anti-MYC antibody.…”

Section: Western Blotting Analysis and Immunoprecipitation (Ip) Assaysmentioning

confidence: 99%

AMPKα1 Regulates Lung and Breast Cancer Progression by Regulating TLR4-Mediated TRAF6-BECN1 Signaling Axis

Kim

Min

Son

et al. 2020

Cancers

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TRAF6-BECN1 signaling axis is critical for autophagy induction and functionally implicated in cancer progression. Here, we report that AMP-activated protein kinase alpha 1 (AMPKα1, PRKAA1) is positively involved in autophagy induction and cancer progression by regulating TRAF6-BECN1 signaling axis. Mechanistically, AMPKα1 interacted with TRAF6 and BECN1. It also enhanced ubiquitination of BECN1 and autophagy induction. AMPKα1-knockout (AMPKα1KO) HEK293T or AMPKα1-knockdown (AMPKα1KD) THP-1 cells showed impaired autophagy induced by serum starvation or TLR4 (Toll-like receptor 4) stimulation. Additionally, AMPKα1KD THP-1 cells showed decreases of autophagy-related and autophagosome-related genes induced by TLR4. AMPKα1KO A549 cells exhibited attenuation of cancer migration and invasion induced by TLR4. Moreover, primary non-small cell lung cancers (NSCLCs, n = 6) with low AMPKαl levels showed markedly decreased expression of genes related to autophagy, cell migration and adhesion/metastasis, inflammation, and TLRs whereas these genes were significantly upregulated in NSCLCs (n = 5) with high AMPKαl levels. Consistently, attenuation of cancer migration and invasion could be observed in AMPKα1KO MDA-MB-231 and AMPKα1KO MCF-7 human breast cancer cells. These results suggest that AMPKα1 plays a pivotal role in cancer progression by regulating the TRAF6-BECN1 signaling axis for autophagy induction.

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“…It also regulates trafficking of many membrane proteins implicated in trans-membrane transport, such as receptors for low-density lipoprotein and transferrin, transporters for amino acids, uric acid, glutamate, glucose, phosphate and cholesterol, and water channels, to control nutrient supply and metabolite discharge (Takata et al, 2008;Gournas et al, 2010;Tachiyama et al, 2011;Koumanov et al, 2012;Sorrentino et al, 2013;Cardona-Lopez et al, 2015;Llinares et al, 2015). It can also regulate trafficking of immune molecules, such as macrophage colony stimulating factor-1, transforming growth factor, Toll/Toll-like and T-cell receptors, and major histocompatibility complex (MHC) class II antigen, to modulate innate and adaptive immunity (Lund and Delotto, 2011;ten Broeke et al, 2013;Lou et al, 2014;Balogh et al, 2015;Wi et al, 2016). Moreover, the MVB pathway can be merged with autophagy for degradation of damaged proteins or subcellular organelles to deal with various stresses (Muller et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Vacuolar protein sorting 4 is required for silkworm metamorphosis

Xia

Chen

Shao

et al. 2019

Insect Molecular Biology

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Vacuolar protein sorting 4 (Vps4) not only functions with its positive regulator vacuolar protein sorting 20‐associated 1 (Vta1) in the multivesicular body (MVB) pathway but also participates alone in MVB‐unrelated cellular processes. However, its physiological roles at the organism level remain rarely explored. We previously identified their respective homologues Bombyx mori Vps4 (BmVps4) and BmVta1 from the silkworm, a model organism for insect research. In this study, we performed fluorescence quantitative real‐time PCR and Western blot to globally characterize the transcription and protein expression profiles of BmVps4 and BmVta1 during silkworm development and in different silkworm tissues and organs. The results showed that they were significantly up‐regulated in metamorphosis, adulthood and embryogenesis relative to larval stages, and displayed a roughly similar tissue‐and‐organ specificity for transcriptions in silkworm larvae. Importantly, BmVps4 was down‐regulated during the early period of the fifth instar, reaching the lowest level of transcription on Day 6, then up‐regulated from Day 7 to the wandering, spinning and pupal stages, and down‐regulated again in adulthood. Moreover, knocking down BmVps4 by RNA interference significantly inhibited silk gland growth, shortened spinning time, prolonged pupation, reduced pupal size and weight, and increased moth wing defects. Together, our data demonstrate the critical and broad requirements for BmVps4 in silkworm metamorphosis.

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Charged MVB protein 5 is involved in T-cell receptor signaling

Cited by 5 publications

References 31 publications

Expression pattern and clinical value of Key RNA methylation modification regulators in ischemic stroke

Expression pattern and clinical value of Key RNA methylation modification regulators in ischemic stroke

AMPKα1 Regulates Lung and Breast Cancer Progression by Regulating TLR4-Mediated TRAF6-BECN1 Signaling Axis

Vacuolar protein sorting 4 is required for silkworm metamorphosis

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