Charged nanoparticles delivery to the eye using hydrogel iontophoresis

Eljarrat-Binstock, Esther; Orucov, Faik; Aldouby, Yanir; Frucht‐Pery, Joseph; Domb, Abraham J.

doi:10.1016/j.jconrel.2007.11.016

Cited by 69 publications

(50 citation statements)

References 32 publications

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“…For that reason, in vitro experiments are undoubtedly an important step Despite this need, in vitro ocular iontophoresis is very poorly evaluated compared to the work that has been done in the transdermal iontophoretic field. Ocular drug absorption is usually studied in vivo using rabbits as the animal model [5,6] . Due to the low volume of aqueous humour in rabbits (less than 200 l) and analytical method limitations, at least 3 animals must be killed per time point to generate a drug concentration profile [7,8] .…”

Section: Introductionmentioning

confidence: 99%

Excised Porcine Cornea Integrity Evaluation in an in vitro Model of Iontophoretic Ocular Research

Gratieri¹,

Gelfuso²,

Thomazini

et al. 2010

Ophthalmic Res

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Background/Aims: It is a challenge to adapt traditional in vitro diffusion experiments to ocular tissue. Thus, the aim of this work was to present experimental evidence on the integrity of the porcine cornea, barrier function and maintenance of electrical properties for 6 h of experiment when the tissue is mounted on an inexpensive and easy-to-use in vitro model for ocular iontophoresis. Methods: A modified Franz diffusion cell containing two ports for the insertion of the electrodes and a receiving compartment that does not need gassing with carbogen was used in the studies. Corneal electron transmission microscopy images were obtained, and diffusion experiments with fluorescent markers were performed to examine the integrity of the barrier function. The preservation of the negatively charged corneal epithelium was verified by the determination of the electro-osmotic flow of a hydrophilic and non-ionized molecule. Results: The diffusion cell was able to maintain the temperature, homogenization, porcine epithelial corneal structure integrity, barrier function and electrical characteristics throughout the 6 h of permeation experiment, without requiring CO₂ gassing when the receiving chamber was filled with 25 mM of HEPES buffer solution. Conclusion: The system described here is inexpensive, easy to handle and reliable as an in vitro model for iontophoretic ocular delivery studies.

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Section: Introductionmentioning

confidence: 99%

Excised Porcine Cornea Integrity Evaluation in an in vitro Model of Iontophoretic Ocular Research

Gratieri¹,

Gelfuso²,

Thomazini

et al. 2010

Ophthalmic Res

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“…Recently iontophoresis has been combined with nanoparticle delivery systems to improve the ocular penetration of nanoparticulate drugs. 84,85 Nanotechnology (nano from the Greek for dwarf) is a term used for technologies that use the properties of nanoscale substances to develop new functions, and it is being used for many applications in the medical field. In the field of drug-delivery systems, nanotechnology is being used in three ways: to enhance absorption and penetration of drugs across membranes; to allow controlled release of the drug; and to target drugs at distinct tissues.…”

Section: Means Of Enhancing Drug Delivery To the Retinamentioning

confidence: 99%

Recent Advances in Topical Therapeutics for Vitreoretinal Diseases

Gibson¹,

McGinnigle

2015

US Ophthalmic Review

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Amongst other attributes, the ideal drug for retinal disorders should be easy to administer, safe, have a long duration of action, and be costeffective. Needless to say, to date, no such drug exists as far as we are aware, but how close does topical delivery come to meeting these aspirations? Compared with intravitreal drug delivery, topical delivery has some obvious advantages and disadvantages and these are summarized in Table 1.Maurice wrote a seminal review of this subject in 2002, in which he was particularly interested in the passage of drugs delivered by eye AbstractEye drops are convenient for patients, but achieving therapeutic doses and maintaining sustained drug release without frequent re-application to treat diseases of the retina has been largely unsuccessful. Topical administration of drugs is hindered by the anatomy, physiology, and biochemistry of the eye and its highly effective defence mechanisms. Advances in nanotechnology have led to the experimental use of topical permeation-enhancing liposomes, emulsions, and microspheres to enhance absorption and penetration of drugs across membranes; allow controlled release of the drug; and to target drugs at distinct tissues to allow sufficient local bioavailability. In the near future it is hoped that improved technologies may provide means of sustained topical drug delivery for retinal therapy, with improved side-effect profiles and reduced cost compared with currently available clinical treatments.

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“…Drug-containing hydrogels, rather than drug solutions, facilitate drug handling, minimize tissue hydration, and allow drug release rate control by changing the characteristics of the hydrogel (118). Recent publications on drug-loaded hydrogels for ocular iontophoresis reported effectiveness in transferring high drug amounts into various eye tissues (95,102,103,106,(119)(120)(121)(122)(123)(124)(125)(126)(127)(128). OcuPhor (Iomed, Inc., Salt Lake City, Utah, USA) is a custom-manufactured hydrogel composed of polyacetal sponge for transscleral iontophoresis (119)(120)(121)(122).…”

Section: The Ocular Iontophoretic Devicementioning

confidence: 99%

“…As described by the authors, iontophoretic delivery of small nanoparticles using 1.5 mA for 5 min revealed strong fluorescent evidence, indicating nanoparticle penetration into ocular tissues, with preference to the positively charged particle. Particle distribution profile revealed a rapid electrical repulsion into the outer ocular tissues (conjunctiva, sclera and cornea) within the first 30 min of treatment, followed by particle migration into the inner tissues (retina and choroid) up to 12 h from treatment (124). Transscleral iontophoresis may be used also for delivering oligonucleotides and genes to posterior eye segments, without losing their physical integrity or biological function.…”

Section: Animal Studiesmentioning

confidence: 99%

New Techniques for Drug Delivery to the Posterior Eye Segment

2010

Self Cite

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Ocular drug delivery has become an increasingly important field of research especially when treating posterior segment diseases of the eye, such as age-related macular degeneration, diabetic retinopathy, posterior uveitis and retinitis. These diseases are the leading causes of vision loss in developed countries which require repeated long-term administration of therapeutic agents. New drugs for the medication of the posterior ocular segment have emerged, but most drugs are delivered by repeated intravitreal injections associated with ocular complications. Advances in ocular drug delivery system research are expected to provide new tools for the treatment of the posterior segment diseases, providing improved drug penetration, prolonged action, higher efficacy, improved safety and less invasive administration, resulting in higher patient compliance. This review provides an insight into the recent progress and trends in ocular drug delivery systems for treating posterior eye segment diseases, with an emphasis on transscleral iontophoresis.

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Charged nanoparticles delivery to the eye using hydrogel iontophoresis

Cited by 69 publications

References 32 publications

Excised Porcine Cornea Integrity Evaluation in an in vitro Model of Iontophoretic Ocular Research

Excised Porcine Cornea Integrity Evaluation in an in vitro Model of Iontophoretic Ocular Research

Recent Advances in Topical Therapeutics for Vitreoretinal Diseases

New Techniques for Drug Delivery to the Posterior Eye Segment

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