We compute the leptonic decay constants f D + , f Ds , and f K + , and the quark-mass ratios m c /m s and m s /m l in unquenched lattice QCD using the experimentally determined value of f π + for normalization. We use the MILC Highly Improved Staggered Quark ( |V cs | = 1.010(5)(18)(6), where the errors are from this calculation of the decay constants, the uncertainty in the experimental decay rates, structure-dependent electromagnetic corrections, and, in the case of |V us |, the uncertainty in |V ud |, respectively.