Charting the Lipopeptidome of Nonpathogenic Pseudomonas

Abstract

Pseudomonas species are prominent producers of lipopeptides that support proliferation in a multitude of environments and foster varied lifestyles. By genome mining of biosynthetic gene clusters (BGCs) with lipopeptide-specific organization, we mapped the global Pseudomonas lipopeptidome and linked its staggering diversity to taxonomy of the producers, belonging to different groups within the major Pseudomonas fluorescens lineage.

Nonribosomal peptides protect Pseudomonas nunensis 4A2e from amoebal and nematodal predation

“…More detailed analyses of nonribosomal lipopeptide families have recently been published elsewhere. 10,11,46…”

“…More detailed analyses of nonribosomal lipopeptide families have recently been published elsewhere. 10,11,46 Structure elucidation of nunapeptin B and C A comparable approach was applied for structure elucidation of peptides 2 and 3. Extensive MS 2 analysis allowed to determine most of the amino acid sequence of both compounds (Fig.…”

Production and characterization of lipopeptide biosurfactant from a new strain ofPseudomonas antarctica28E using crude glycerol as a carbon source

“…14 In fact, the extensively studied group of viscosins has so far included 17 characterized compounds, represented by viscosin, viscosinamide, WLIP (white line-inducing principle) pseudodesmin, massetolide and pseudophomins. 15 These lipopeptides are composed of a peptide chain of nine amino acids, typically attached to 3-hydroxydecanoic acid (3-HDA). 15 The structure is cyclized by the ester bound, which connects the C-terminus of the peptide chain with the alcohol function of the third threonine side chain.…”

“…15 These lipopeptides are composed of a peptide chain of nine amino acids, typically attached to 3-hydroxydecanoic acid (3-HDA). 15 The structure is cyclized by the ester bound, which connects the C-terminus of the peptide chain with the alcohol function of the third threonine side chain. Viscosin-like lipopeptides are varied in terms of the hydrophobic amino acid located at positions 4 and 9, where Leu, Ile or Val may be present.…”

“…In addition, the fatty acid tail may include from 10 to 12 carbon atoms. 15,16 To date, viscosin production has been demonstrated for a limited number of Pseudomonas species. The rst report of Groupé et al 17 described the isolation of the antibiotic compound, and its activity against pathogenic and saprophytic mycobacteria.…”