This paper presents a new control strategy for vehicle stability based on brake-by-wire. However, there are few studies in the literature that compare the stability of a vehicle by systematic experimentation with or without controllers. In this paper, the complete experimental procedure is designed, and the experimental results are analyzed in detail. Firstly, the hydraulic model of the brake-by-wire is established based on its structure and working principles, and the yaw moment control method is proposed for the vehicle’s stability. The deviation between the desired values and actual values of the yaw rate and sideslip angle is taken as the input, and the fuzzy controller calculates the additional yaw moment for the vehicle stability. Next, the simulation under different conditions which contain the steering wheel step input, double lane change and turning is conducted, and the yaw rates and sideslip angles with and without stability control are compared, and the effectiveness of the control method is verified. Finally, the turning test is conducted based on brake-by-wire chassis to verify the proposed method. The experimental results show that the yaw rate decreased by 14% and the sideslip angle decreased by 25% when the brake control was applied. Furthermore, the proposed method performed well in improving the stability of the brake-by-wire chassis.