Chatuphalatika, a Thai herbal formula, ameliorate obesity and dyslipidemia in high-fat diet fed mice

Abstract

Background Chatuphalatika is a Thai traditional health tonic composing of four different herbs namely Terminalia bellerica Linn., T. chebula Retz., T. arjuna Roxb. and Phyllanthus emblica Linn. The fact that phytoconstituents of Terminalia species have been reported to ameliorate obesity and symptoms of metabolic syndrome prompt us to investigate acute toxicity as well as a lipid lowering activity of orally given chatuphalatika aqueous extract (CPT) in animal models. Methods CPT was prepared by decoction method and the phytochemical contents were quantified by HPLC analysis. The acute oral toxicity study of CPT was performed in Wistar rats following the protocol of the Organisation for Economic Co-operation and Development (OECD) guidelines No. 420. The assessment of lipid-lowering effect of CPT was carried out in high-fat diet (HFD) fed C57BL/6 mice model. Results Gallic acid was the highest content (137.10 ± 5.42 mg/g) found in CPT followed by chebulinic acid (73.60 ± 2.35 mg/g), chebulagic acid (62.60 ± 4.17 mg/g) and ellagic acid (5.2 ± 0.40 mg/g). No lethality and no signs of toxicity were observed in either male or female rats orally treated with CPT at a single dose of 2,000 and 5,000 mg/kg. Daily oral administration of 250, 500 or 1,000 mg/kg of CPT for 6 weeks to the HFD-induced dyslipidemia mice markedly reduced low density lipoprotein cholesterol (LDL-C) levels and increased high density lipoprotein cholesterol (HDL-C) levels, whereas the significant reduction of total cholesterol (TC) levels was observed only in the high doses of 500 and 1,000 mg/kg of CPT. Moreover, dyslipidemia mice received 1,000 mg/kg CPT exclusively showed the decrease in body weight gain. Conclusions The CPT was safe and effective for relieving dyslipidemia and reducing weight gain in animal model supporting further development as functional food for dyslipidemia or overweight population.

Phytochemical Analysis, Antimalarial Properties, and Acute Toxicity of Aqueous Extracts of Trisamo and Jatu-Phala-Tiga Recipes

“…JPT is well known for its antioxidant activity, and it is used as an antipyretic, laxative, stomachic, colon cleanser, detoxifying agent, health promotion agent, and rejuvenator in Tai traditional medicine [13,14]. Scientifc evidence has revealed that JPT has antimutagenic, cardioprotective, radioprotective, hepatoprotective, anti-infammatory, and antiobesity properties [15,16]. In addition, the TSM and JPT recipes consist of numerous secondary metabolites such as favones, alkaloids, phenols, tannins, coumarin, terpenoids, glycosides, and saponins [17][18][19].…”