CHD4 orchestrates the symphony of T and B lymphocytes development and a good mediator in preventing from autoimmune disease

Jia, Miaomiao; Zou, Xueqin; Yin, Shuying; Tian, Weihong; Zhao, Yangjing; Wang, Hui; Xu, Guoying; Cai, Wei; Shao, Qixiang

doi:10.1002/iid3.644

Cited by 4 publications

(1 citation statement)

References 46 publications

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“…The ATP-dependent chromatin remodeler proteins are implicated in many developmental disorders, including hearing loss [ 2 , 3 ]. Normal CHD4 activity was found to be essential for maintaining stem cell pluripotency [ 4 ], differentiation capacity towards neuronal lineage [ 5 ], T cell development and hematopoietic stem cell [ 6 , 7 ], cardiac development [ 8 , 9 ], renal progenitor cell self-renewal, and differentiation [ 10 ]. It has also been shown that CHD4 can regulate gene expression for maintaining a distinct cellular state and pledge for accurate cell fate decisions upon developmental/external cues [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Generation of a Well-Characterized Homozygous Chromodomain-Helicase-DNA-Binding Protein 4G1003D Mutant hESC Line Using CRISPR/eCas9 (ULIEGEe001-A-1)

Chohra¹,

Giri²,

Malgrange³

2023

IJMS

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The chromatin remodeler Chromodomain-helicase-DNA-binding protein 4 (CHD4) is crucial for the development of multiple organ systems. Functional mutations of CHD4 have recently been described in a developmental disorder, namely Siffrim-Hitz-Weiss syndrome (SIHIWES). Herein, we have generated a homozygous CHD4G1003D hESC line (WAe025-A-1) using CRISPR/eCas9-based gene editing in the WA-25 hESC line. The edited hESC line maintains normal karyotype, pluripotency, and ability to differentiate into three germ layers. This cell line will be a valuable resource for studying the functional role of CHD4 during the development and disease modeling of SIHIWES in vitro.

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Section: Introductionmentioning

confidence: 99%

Generation of a Well-Characterized Homozygous Chromodomain-Helicase-DNA-Binding Protein 4G1003D Mutant hESC Line Using CRISPR/eCas9 (ULIEGEe001-A-1)

Chohra¹,

Giri²,

Malgrange³

2023

IJMS

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Dysregulation of epigenetic modifications in inborn errors of immunity

Xiao,

He,

Zhao

et al. 2024

Epigenomics

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Distinct deregulation trends of transcriptional protein complexes in aging naive T cells

Kökrek,

Pir

2024

Journal of Leukocyte Biology

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The impact of aging on T cell subsets, specifically CD4+ and CD8+ T cells, leading to immune system dysfunction has been the focus of scientific investigation due to its potential to reverse age-associated deterioration. Transcriptomic and epigenomic studies have identified the primary regulators in T cell aging. However, comprehending the underlying dynamic mechanisms requires studying these proteins with their interactors. Here, we integrated single-cell RNA sequencing data of naive CD4+ and CD8+ T cells obtained from 3 different age groups with protein-protein and domain-domain interaction networks to predict and compare the transcriptional protein complexes and identify their capacity to explain age-associated variances. Our novel approach revealed significant effects of aging on the repertoire of complexes, which remains unchanged in naive CD4+ T cells, while in naive CD8+ T cells, it diminishes. In both cell types, there was major deregulation of complexes with the same composition, involving a range of transcription factors. This aging-associated deregulation is characterized by a specific set of protein complexes in naive CD4+ T cells, but this pattern is not observed in naive CD8+ T cells. SMAD3 and BCL11A complexes emerge as key markers in defining a trajectory in aging naive CD4+ T cells. These complexes can accurately distinguish between 3 different age groups, indicating their potential as targets. The direct link between SMAD3 and FOS complexes whose regulatory role has been previously implicated in aging and MBD3 as the novel key link between SMAD3 and BCL11A complexes implicates a coordinated mechanism in age-associated deregulation.

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CHD4 orchestrates the symphony of T and B lymphocytes development and a good mediator in preventing from autoimmune disease

Cited by 4 publications

References 46 publications

Generation of a Well-Characterized Homozygous Chromodomain-Helicase-DNA-Binding Protein 4G1003D Mutant hESC Line Using CRISPR/eCas9 (ULIEGEe001-A-1)

Generation of a Well-Characterized Homozygous Chromodomain-Helicase-DNA-Binding Protein 4G1003D Mutant hESC Line Using CRISPR/eCas9 (ULIEGEe001-A-1)

Dysregulation of epigenetic modifications in inborn errors of immunity

Distinct deregulation trends of transcriptional protein complexes in aging naive T cells

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