The effect of green and brown seaweeds supplementation on laying performance, egg quality, serum lipid profile and antioxidant capacity was evaluated in Japanese quails. Five groups of Japanese quails (total = 180; 120 hens + 60 males) of 10 wks old were fed on diets without or with green (GS) and brown (BS) seaweeds, each at 1.5 and 3% for 14 weeks. Results indicate that GS and BS supplementation significantly improved egg-laying rate and increase egg number, egg weight and egg mass and decrease feed conversion ratio (FCR). However, feed consumption (FC) was not significantly affected by experimental treatments. The GS and BS supplemented groups led to improve the eggshell thickness, also helped to improve the egg yolk weight, index and color compared to the control. Supplementing dried GS and BS significantly decreased the value of Haugh unit, total lipids and total cholesterol content of egg yolk as well as displayed higher fertility and hatchability percentages than those of the control group. Quails received dried GS and BS treatments had a higher dressing percentage and a lesser percentage of abdominal fat compared to the control group. Serum total lipids, triglycerides, total cholesterol, HDL and LDL values were significantly decreased compared to the control. There were significant effects on enzymatic antioxidant activity of blood serum (malondialdehyde, total antioxidant capacity, catalase, glutathione peroxidase and superoxide dismutase). It is concluded that green and brown seaweeds supplementation can improve egg-laying performance and had a positive effect on fertility and hatchability values and resulted in significant reduction in total lipids and total cholesterol in the serum and yolk while enhancing total antioxidant capacity in blood serum as well as positively affecting economic parameters. Thus, these results suggested that green and brown seaweeds can be used safely as a feed additive in diets for laying Japanese quail.