“…Based on the morphologic and genetic pattern observed in the present study, the specimens with a black blotch on dorsal fin obtained from Chalakkudy, Periyar and Pamba river systems (CHD, PER(c) and PMB samples, respectively), could be considered as P. chalakkudiensis. Thus, the distribution of P. chalakkudiensis should be extended to Periyar and Pamba River systems, in addition to the previously reported type locality, the Chalakkudy River [1] and Achankovil [48]. The rest of the haplotypes of P. denisonii, without a black blotch on dorsal fin, obtained from four different river systems of north Kerala (CDR, KGD, VLP and CHR samples) and from Periyar river [PER(d)], were further separated into four distinct well separated monophyletic clades (C1, C2, C3 and C4), with high level of genetic ( Figure 5; Table 7), and morphologic divergence (Figure 4b; Table 3), indicating the presence of four independent evolutionary lineages within the species.…”