Introduction: Cheiloscopy is as effective as dactyloscopy in identification methods because each person has a lip pattern distinguishable from others (unique, invariable, permanent and classifiable). The aim of this study is to identify the most frequent lip patterns in men and women using the Suzuki and Tsuchihashi classification, thus establishing a guide to verify the usefulness of this area in identification. Method: cross-sectional observational study (n=121), analysis of the lip pattern in the lower middle third of the lower third of the labia of the Suzuki and Tsuchihashi classification (I, I’, II, III, IV and V) with print on A4 paper and photographs Canon EOS 1300D (W) digital SLR camera with CMOS sensor with 18.0 megapixels. Double-blind and Chi squared statistical analysis was performed, with independent variable sex and dependent variable cheiloscopy path. Results: Regarding the total sample: women (n= 61; 50.41%) of type I; men (n= 28; 23.14%). Type I’ men (n=5; 6.05%), women (n=14; 16.94%). In type II we find in Type II and III in males the same proportion (n=2; 2.42%) and females in type II (3.63%) and type III (1.21%), Type V females (n=5; 6.05%), Males n= 0; in type IV there are no individuals with this pattern. There are no statistically significant differences between sex differentiation and cheiloscopic pattern, with a P>0.05. Conclusions: There is weak evidence to be able to use cheiloscopy as a method of sex-cheiloscopic pattern differentiation. The predominant pattern in women is I, I’ and V, in men I and I’. There is no type IV and type V only occurs in women.