Chelating agents improve enzymatic solubilization of pectinaceous co-processing streams

Ravn, Helle Christine; Meyer, Anne S.

doi:10.1016/j.procbio.2013.11.010

Cited by 17 publications

(10 citation statements)

References 43 publications

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“…Monosaccharide composition analysis was done as described by Ravn and Meyer (37). In brief, samples were hydrolyzed by trifluoroacetic acid (soluble carbohydrate) or sulfuric acid (potato pulp), followed by quantification on a high-pH anion-exchange chromatography (HPAEC)-pulsed amperometric detection (PAD) system using a Dionex CarboPac PA1 analytical column (2 mm by 250 mm) combined with a CarboPac PA1 precolumn (2 mm by 50 mm) and 0.25 to 500 mM NaOH (37). Rhamnose, arabinose, galactose, glucose, mannose, xylose, and galacturonic acid were included as standards.…”

Section: Methodsmentioning

confidence: 99%

In Situ Prebiotics for Weaning Piglets: In Vitro Production and Fermentation of Potato Galacto-Rhamnogalacturonan

Strube

Ravn

Ingerslev

et al. 2015

Appl Environ Microbiol

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Postweaning diarrhea (PWD) in pigs is a leading cause of economic loss in pork production worldwide. The current practice of using antibiotics and zinc to treat PWD is unsustainable due to the potential of antibiotic resistance and ecological disturbance, and novel methods are required. In this study, an in vitro model was used to test the possibility of producing prebiotic fiber in situ in the gastrointestinal (GI) tract of the piglet and the prebiotic activity of the resulting fiber in the terminal ileum. Soluble fiber was successfully produced from potato pulp, an industrial waste product, with the minimal enzyme dose in a simulated upper GI tract model extracting 26.9% of the initial dry matter. The fiber was rich in galactose and galacturonic acid and was fermented at 2.5, 5, or 10 g/liter in a glucose-free medium inoculated with the gut contents of piglet terminal ileum. Fermentations of 5 g/liter inulin or 5 g/liter of a purified potato fiber were used as controls. The fibers showed high fermentability, evident by a dose-dependent drop in pH and an increase in the organic acid content, with lactate in particular being increased. Deep sequencing showed a significant increase in the numbers of Lactobacillus and Veillonella organisms and an insignificant increase in the numbers of Clostridium organisms as well as a decrease in the numbers of Streptococcus organisms. Multivariate analysis showed clustering of the treatment groups, with the group treated with purified potato fiber being clearly separated from the other groups, as the microbiota composition was 60% Lactobacillus and almost free of Clostridium. For animal studies, a dosage corresponding to the 5-g/liter treatment is suggested.

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Section: Methodsmentioning

confidence: 99%

In Situ Prebiotics for Weaning Piglets: In Vitro Production and Fermentation of Potato Galacto-Rhamnogalacturonan

Strube

Ravn

Ingerslev

et al. 2015

Appl Environ Microbiol

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“…smaller molecules and salts. The retentate was freeze-dried and subjected to sulfuric acid hydrolysis as described previously [28] and monosaccharide composition was determined by HPAEC-PAD.…”

Section: Preparation Of Xyg From Citrus Peelmentioning

confidence: 99%

Substrate specificity and transfucosylation activity of GH29 α-l-fucosidases for enzymatic production of human milk oligosaccharides

et al. 2018

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Human milk oligosaccharides (HMOs) constitute a unique family of bioactive lactose-based molecules present in human breast milk. HMOs are of major importance for infant health and development but also virtually absent from bovine milk used for infant formula. Among the HMOs, the fucosylated species are the most abundant. Transfucosylation catalysed by retaining α-l-fucosidases is a new route for manufacturing biomimetic HMOs. Seven α-l-fucosidases from glycosyl hydrolase family 29 were expressed, characterized in terms of substrate specificity and thermal stability, and shown to be able to catalyse transfucosylation. The α-l-1,3/4-fucosidase CpAfc2 from Clostridium perfringens efficiently catalysed the formation of the more complex human milk oligosaccharide structure lacto-N-fucopentaose II (LNFP II) using 3-fucosyllactose as fucosyl donor and lacto-N-tetraose as acceptor with a 39% yield. α-l-Fucosidases FgFCO1 from Fusarium graminearum and Mfuc5 from a soil metagenome were able to catalyse transfucosylation of lactose using citrus xyloglucan as fucosyl donor. FgFCO1 catalysed formation of 2'-fucosyllactose, whereas Mfuc5 catalysis mainly produced an unidentified, non-HMO fucosyllactose, reaching molar yields based on the donor substrate of 14% and 18%, respectively.

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“…4 The extraction with chelators, on the other hand, liberates calcium-bound pectin by loosening the egg box structures formed by homogalacturonan and calcium complex. 5 A major challenge in pectin recovery from residues concerns the extraction process used for its isolation. Previous studies have indicated that the extraction process can cause a high degree of pectin degradation, resulting in a low yield and a loss of pectin functionality, that is, gelling property.…”

Section: Introductionmentioning

confidence: 99%

“…The principle involves the hydrolysis of the protopectin, which results in diffusion of pectin from the cell walls . The extraction with chelators, on the other hand, liberates calcium-bound pectin by loosening the egg box structures formed by homogalacturonan and calcium complex …”

Section: Introductionmentioning

confidence: 99%

Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues

Babbar

Roy

Wijnants

et al. 2015

J. Agric. Food Chem.

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The influence of different extraction methodologies was assessed on the composition of both neutral (arabinose, rhamnose, galactose) and acidic (galacturonic acid) pectic polysaccharides obtained from four agro-industrial residues, namely, berry pomace (BP), onion hulls (OH), pressed pumpkin (PP), and sugar beet pulp (SBP). For acidic pectic polysaccharides, the extraction efficiency was obtained as BP (nitric acid-assisted extraction, 2 h, 62.9%), PP (enzymatic-assisted extraction, 12 h, 75.0%), SBP (enzymatic-assisted extraction, 48 h, 89.8%; and nitric acid-assisted extraction, 4 h, 76.5%), and OH (sodium hexametaphosphate-assisted extraction, 0.5 h, 100%; and ammonium oxalate-assisted extraction, 0.5 h, 100%). For neutral pectic polysaccharides, the following results were achieved: BP (enzymatic-assisted extraction, 24 h, 85.9%), PP (nitric acid-assisted extraction, 6 h, 82.2%), and SBP (enzymatic assisted extraction, 48 h, 97.5%; and nitric acid-assisted extraction, 4 h, 83.2%). On the basis of the high recovery of pectic sugars, SBP and OH are interesting candidates for the further purification of pectin and production of pectin-derived products.

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Chelating agents improve enzymatic solubilization of pectinaceous co-processing streams

Cited by 17 publications

References 43 publications

In Situ Prebiotics for Weaning Piglets: In Vitro Production and Fermentation of Potato Galacto-Rhamnogalacturonan

In Situ Prebiotics for Weaning Piglets: In Vitro Production and Fermentation of Potato Galacto-Rhamnogalacturonan

Substrate specificity and transfucosylation activity of GH29 α-l-fucosidases for enzymatic production of human milk oligosaccharides

Effect of Extraction Conditions on the Saccharide (Neutral and Acidic) Composition of the Crude Pectic Extract from Various Agro-Industrial Residues

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