Chelator Iminodisuccinic Acid Regulates Reactive Oxygen Species Accumulation and Improves Maize (Zea mays L.) Seed Germination under Pb Stress

Zhang, Yifei; Sun, Yishan; Li, Weiqing; Li, Jiayu; Xu, Rongqiong; Du, Jiarui; Li, Zesong; Li, Guibin; Yang, Kejun

doi:10.3390/plants11192487

Cited by 8 publications

(4 citation statements)

References 68 publications

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“…Indeed, EDTA unchelated with trace elements inhibits cell division, chlorophyll synthesis, alters cell biochemical processes and leads to necrotic leaf lesions [40,53,54]. In the study of Zhang et al [54], it was shown that with an increase in IDS concentration (from 5 to 100 mmol/L), corn seed germination was slowed down and the length of seedlings was reduced; in the presence of Pb IDS, conversely, the length of corn seedlings was 20-67% higher. The introduction of IDS as a fertilizer into the soil may lead to its entry not only into the soil but also into aquatic ecosystems.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation Rate of EDTA and IDS and Their Metal Complexes

Beltyukova,

Kuryntseva,

Galitskaya

et al. 2023

Horticulturae

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Ethylenediaminetetraacetic acid (EDTA), when used as a main chelator for complex plant microfertilizers, causes many negative environmental effects; therefore, new compounds or new use of the known compounds to replace EDTA have been widely studied. In the present study, biodegradation rate, plant (Raphanus sativus) growth stimulation and ecotoxicity towards Daphnia magna and Chlorella vulgaris of iminodisuccinic acid (IDS), considered as an alternative for EDTA in agriculture, has been investigated. It was demonstrated that IDS’ biodegradation rate over 28 days was 28.1%, which is 4.5 times higher than that of EDTA. Similar to EDTA, complexation with metals led to an increase in the degradation rate by 1.8-fold. The majority of compounds were degraded within first 7 days. The GI values for IDS implemented at concentrations of 100 mg/L (both in pure form and in combination with microelements) were 2.4–2.6 times higher than those of EDTA. The ecotoxicity index EC10 of IDS was estimated to be 2.0 g/L and 5.8 g/L towards D. magna and Ch. vulgaris which are 4.1- and 10-fold higher than those for EDTA, respectively. It can be concluded that IDS is a promising agent to chelate microelements used in plant nutrition.

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Section: Discussionmentioning

confidence: 99%

Biodegradation Rate of EDTA and IDS and Their Metal Complexes

Beltyukova,

Kuryntseva,

Galitskaya

et al. 2023

Horticulturae

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“…The accumulation of Pb inhibits the germination and growth of plants [14]. Pb stress induces deterioration of seed germination, as reported in many plants, such as Brassica juncea [15], Phaseolus vulgaris [16], and Zea mays [17]. When plants are exposed to Pb stress, the development of roots and aerial parts is attenuated, especially in roots, probably due to increased Pb accumulation [18,19].…”

Section: Introductionmentioning

confidence: 99%

Flavonoid metabolism plays an important role in response to Pb stress in maize at seedling stage

Han,

Zheng,

Zhang

et al. 2024

Preprint

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Pb stress, a toxic abiotic stress, critically affects maize production and food security. Although some progress has been made in understanding the damage caused by Pb stress and plant response strategies, the regulatory mechanisms and resistance genes involved in the response to lead stress in crops are largely unknown. In this study, the response mechanism of maize to Pb stress, the expression of Pb tolerance genes, physiological and biochemical indexes, the transcriptome, and the metabolome under different concentrations of Pb stress were combined for comprehensive analysis. As a result, the antioxidant system was significantly inhibited under Pb stress, especially under relatively high Pb concentrations. Transcriptome analysis revealed 3559 co-DEGs under the four Pb concentration treatments, which were enriched mainly in the GO terms related to DNA-binding transcription factor activity, response to stress, response to reactive oxygen species, cell death, the plasma membrane and root epidermal cell differentiation. Metabolome analysis revealed 72 and 107 DEMs under T500 and T2000, respectively, and 36 co-DEMs. KEGG analysis of the DEMs and DEGs revealed a common metabolic pathway, namely, flavonoid biosynthesis. An association study between the flavonoid biosynthesis-related DEMs and DEGs revealed 20 genes associated with flavonoid-related metabolites, including 3 for genistin and 17 for calycosin. This study not only provides genetic resources for the genetic improvement of maize Pb tolerance but also enriches the theoretical basis of the maize Pb stress response.

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“…ROS production and microglial cell activation are obviously inhibited by pretreatment with DMTU [38].Notably, DMTU is considered the least toxic of alkylthioureas and is widely used in in vivo studies because of its low toxicity to biological systems [39,40]. In plant systems, DMTU was often used in previous studies to determine ROS homeostasis mechanisms during plant growth and development in adversity [41][42][43]. Furthermore, DMTU treatment can alleviate the effects of exogenous spermidine (Spd) on maize seedling growth to some extent [44].…”

Section: Introductionmentioning

confidence: 99%

Effect of Reactive Oxygen Scavenger N,N′-Dimethylthiourea (DMTU) on Seed Germination and Radicle Elongation of Maize

Li,

Zhang

et al. 2023

IJMS

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Reactive oxygen species (ROS) are an important part of adaptation to biotic and abiotic stresses and regulate seed germination through positive or negative signaling. Seed adaptation to abiotic stress may be mediated by hydrogen peroxide (H2O2). The effects of the ROS scavenger N,N′-dimethylthiourea (DMTU) on maize seed germination through endogenous H2O2 regulation is unclear. In this study, we investigated the effects of different doses of DMTU on seed endogenous H2O2 and radicle development parameters using two maize varieties (ZD958 and DMY1). The inhibitory effect of DMTU on the germination rate and radicle growth was dose-dependent. The inhibitory effect of DMTU on radicle growth ceased after transferring maize seeds from DMTU to a water medium. Histochemical analyses showed that DMTU eliminated stable H2O2 accumulation in the radicle sheaths and radicles. The activity of antioxidant enzyme and the expression of antioxidant enzyme-related genes (ZmAPX2 and ZmCAT2) were reduced in maize seeds cultured with DMTU compared with normal culture conditions (0 mmol·dm−3 DMTU). We suggest the use of 200 mmol·dm−3 DMTU as an H2O2 scavenger to study the ROS equilibrium mechanisms during the germination of maize seeds, assisting in the future with the efficient development of plant growth regulators to enhance the seed germination performance of test maize varieties under abiotic stress.

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Chelator Iminodisuccinic Acid Regulates Reactive Oxygen Species Accumulation and Improves Maize (Zea mays L.) Seed Germination under Pb Stress

Cited by 8 publications

References 68 publications

Biodegradation Rate of EDTA and IDS and Their Metal Complexes

Biodegradation Rate of EDTA and IDS and Their Metal Complexes

Flavonoid metabolism plays an important role in response to Pb stress in maize at seedling stage

Effect of Reactive Oxygen Scavenger N,N′-Dimethylthiourea (DMTU) on Seed Germination and Radicle Elongation of Maize

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