Chemical activation of egg shell membrane for covalent immobilization of enzymes and its evaluation as inert support in urinary oxalate determination

Pundir, C.S.; Bhambi, Manu; Chauhan, Nar Singh

doi:10.1016/j.talanta.2008.10.004

Cited by 59 publications

(28 citation statements)

References 18 publications

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“…[6][7][8] Mainly, the methods are divided into 2 categories: covalent bonding and entrapping. While the possibility of loss of the immobilized enzyme exists, through leakage or dissolution, it is small for immobilization achieved by covalent bonding [9][10][11] and cross-linking, [12][13][14][15][16][17][18][19][20] and there remains fear of enzyme activity loss due to reagents used in the bonding reaction; there are means for prolonging the duration of enzyme stability, but the enzyme may be damaged during such immobilization processes.…”

Section: Introductionmentioning

confidence: 99%

Preparation of a Cellulose-based Enzyme Membrane Using Ionic Liquid to Lengthen the Duration of Enzyme Stability

et al. 2012

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Section: Introductionmentioning

confidence: 99%

Preparation of a Cellulose-based Enzyme Membrane Using Ionic Liquid to Lengthen the Duration of Enzyme Stability

et al. 2012

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“…The stabilities of the immobilized lipases were obviously improved on these natural polymeric membranes. In recent studies, egg shell membranes, which are a novel, robust, microporous, cost effective, easily available organic support material, were also utilized for lipase immobilization (38). It was found that the immobilized enzymes were stable even after 180 days of storage.…”

Section: Enzyme Immobilization On Membranesmentioning

confidence: 99%

Polymer materials for enzyme immobilization and their application in bioreactors

Fang¹,

Huang²,

Chen³

et al. 2011

BMB Reports

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Enzymatic catalysis has been pursued extensively in a wide range of important chemical processes for their unparalleled selectivity and mild reaction conditions. However, enzymes are usually costly and easy to inactivate in their free forms. Immobilization is the key to optimizing the in-service performance of an enzyme in industrial processes, particularly in the field of non-aqueous phase catalysis. Since the immobilization process for enzymes will inevitably result in some loss of activity, improving the activity retention of the immobilized enzyme is critical. To some extent, the performance of an immobilized enzyme is mainly governed by the supports used for immobilization, thus it is important to fully understand the properties of supporting materials and immobilization processes. In recent years, there has been growing concern in using polymeric materials as supports for their good mechanical and easily adjustable properties. Furthermore, a great many work has been done in order to improve the activity retention and stabilities of immobilized enzymes. Some introduce a spacer arm onto the support surface to improve the enzyme mobility. The support surface is also modified towards biocompatibility to reduce non-biospecific interactions between the enzyme and support. Besides, natural materials can be used directly as supporting materials owning to their inert and biocompatible properties. This review is focused on recent advances in using polymeric materials as hosts for lipase immobilization by two different methods, surface attachment and encapsulation. Polymeric materials of different forms, such as particles, membranes and nanofibers, are discussed in detail. The prospective applications of immobilized enzymes, especially the enzyme-immobilized membrane bioreactors (EMBR) are also discussed. [BMB reports 2011; 44(2): 87-95]

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“…It is important to note that eggshells are abundant and environmental friendly [25]. In this paper, our objective is to assess the effectiveness of activated carbon derived from eggshell waste as an adsorbent of dissolved H 2 S from waste water.…”

Section: Introductionmentioning

confidence: 99%

Isothermal Modelling Based Experimental Study of Dissolved Hydrogen Sulfide Adsorption From Waste Water Using Eggshell Based Activated Carbon

Habeeb¹,

Ramesh²,

Ali³

et al. 2017

MJAS

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This paper reports on the experimental work using batch process conducted to determine the adsorption capacity of dissolved hydrogen sulfide in the synthetic wastewater onto the activated carbon which is derived from the eggshell. Fourier Transform Infrared Spectroscopy (FTIR), Energy-Dispersive X-ray Spectroscopy (EDX), Scanning Electron Microscopy (SEM), and particle size distribution have been used to characterize the prepared material. The raw materials of chicken eggshell are adopted to retrieve the carbon content which is then activated using KOH as the activation agent. The examined concentration of dissolved hydrogen sulfide is ranging from 100 to 500 ppm. The maximum adsorption capacity of the dissolved H 2 S onto the activated carbon is 289.3 mg/g and the equilibrium time is 6 hours. The examined pH value in this study is ranging from 4.5 to 5.5. The two well-known equilibrium adsorption isotherm models, i.e. the Langmuir and the Freundlich models, are employed. It is found that the adsorption isotherm capacity agrees very well to the Freundlich isotherm model. This paper attempts to show the difficulties of converting CaCO 3 to carbon due to the fact that the raw material contains higher calcium (Ca) content instead of carbon. It is concluded that the carbon derived from the chickens' eggshells is very beneficial for treatment of dissolved H 2 S in waste water. Keywords: adsorption, hydrogen sulfide, chicken eggshells, activated carbon, isotherm AbstrakKajian ini melaporkan mengenai kerja eksperimen menggunakan proses kelompok yang dijalankan untuk menentukan kapasiti penjerapan hidrogen sulfida terlarut dalam air sisa sintetik ke dalam karbon yang diaktifkan dihasilkan daripada kulit telur. Spektroskopi Inframerah Fourier (FTIR), Spektroskopi X-ray Tenaga Serakan (EDX), Mikroskop Imbasan Elektron (SEM), dan taburan saiz zarah telah digunakan untuk mencirikan bahan yang disediakan. Kulit telur ayam sebagai bahan mentah telah digunakan untuk mendapatkan semula kandungan karbon yang kemudiannya diaktifkan dengan menggunakan KOH sebagai agen pengaktifan. Julat kepekatan hidrogen sulfida yang diperiksa adalah di antara 100 hingga 500 ppm. Kapasiti penjerapan maksimum H 2 S terlarut ke dalam karbon diaktifkan adalah 289.3 mg/g dan masa keseimbangan selama 6 jam. Julat nilai pH yang diperiksa dalam kajian ini di antara 4.5 hingga 5.5. Kedua-dua model penjerapan isoterma keseimbangan terkenal seperti model Freundlich dan Langmuir telah dilaksanakan. Kapasiti penjerapan isoterma didapati selari dengan model isoterma Langmuir. Kajian ini cuba menunjukkan kesukaran untuk menukar CaCO 3 kepada karbon disebabkan oleh bahan mentah

show abstract

Chemical activation of egg shell membrane for covalent immobilization of enzymes and its evaluation as inert support in urinary oxalate determination

Cited by 59 publications

References 18 publications

Preparation of a Cellulose-based Enzyme Membrane Using Ionic Liquid to Lengthen the Duration of Enzyme Stability

Preparation of a Cellulose-based Enzyme Membrane Using Ionic Liquid to Lengthen the Duration of Enzyme Stability

Polymer materials for enzyme immobilization and their application in bioreactors

Isothermal Modelling Based Experimental Study of Dissolved Hydrogen Sulfide Adsorption From Waste Water Using Eggshell Based Activated Carbon

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