Chemical and Biological insights of Ouratea hexasperma (A. St.-Hil.) Baill.: a source of bioactive compounds with multifunctional properties

Abstract

The study aimed to evaluate in vitro antioxidant, anticholinesterase and antidiabetic properties of Ouratea hexasperma (A. St.-Hil.) Baill. The inflorescence methanol extract and the ethyl acetate fraction of leaves and stems reported the highest Relative Antioxidant Capacity Index (RACI), whereas the dichloromethane fraction of leaves was the best inhibitor of α-amylase and α-glucosidase. Trans-3-O-methyl-resveratrol-2-C-β-glucoside, lithospermoside, 2,5-dimethoxy-p-benzoquinone, lup-20(30)-ene-3β,28-diol, 7-O-methylgenistein, apigenin and luteolin and amentoflavone were isolated from O. hexasperma. Resveratrol derivative was isolated for the first time in Ochnaceae family. Luteolin, followed by apigenin, reported the highest Relative Antioxidant Capacity Index and they were also the best inhibitors of α-glucosidase enzyme.

Chemical analysis and antioxidant properties of orange‐tree (Citrus sinensis L.) biomass extracts obtained via different extraction techniques

“…The reduction is monitored by measuring the change of absorbance at 593 nm. Increased absorbance of the reaction mixture indicates an increase of reduction capability . As reported by Todaro et al ., the FRAP reagent was prepared daily with a 300 mmol L acetate buffer (pH 3.6), 20 mmol L –1 ferric chloride, and 10 mmol L –1 TPTZ (in 40 mmol L –1 hydrochloric acid) at a ratio of 10 : 1 : 1.…”

Antioxidant Activity and Phytochemical Characterization of Senecio clivicolus Wedd.

“…Lipid peroxidation inhibition was evaluated by the β -carotene bleaching method (BCB) [ 57 ]. The β -Carotene emulsion (950.00 µL) was mixed with 50.00 µL of sample at 1.00 mg/mL and BHT was used as positive control, then the mixture (250.00 µL) was transferred into a 96-well plate.…”

Phytochemicals of Minthostachys diffusa Epling and Their Health-Promoting Bioactivities

“…The α-amylase enzyme from hog pancreas was mixed with different concentrations of each sample and starch used as substrates [23]. Briefly, the aromatic yellow-orange 3,5-dinitrosalicylic acid reagent was added that reacts with reducing sugars released from starch hydrolysis and other reducing molecules to form 3-amino-5-nitrosalicylic acid, which was subsequently monitored at 540 nm.…”

“…The inhibition of α-glucosidase enzyme was performed as previously described [23]. The α-glucosidase enzyme, the substrate 4-p-nitrophenyl-α-d-glucopyranoside, and different concentrations of each sample were mixed, and the reaction was spectrophotometrically monitored at 405 nm by the release of yellow p-nitrophenol.…”