Chemical and Biological Investigations of Allium scorodoprasum L. Flower Extracts

Abstract

This study was designed to investigate the impact of different extraction solvent systems on the chemical composition and biological activities of Allium scorodoprasum L. (Amaryllidaceae)—the medicinal plant that was traditionally used as a remedy in the medieval period in the Balkans. Targeted chemical analysis of nine different extracts was performed by UHPLC(−)HESI–QqQ-MS/MS. Antimicrobial and antibiofilm activities of the extracts were investigated on sixteen clinical isolates of bacteria, yeasts and dermatomycetes, all isolated from infected human skin and corneal formations. Cytotoxicity and wound-healing properties were tested on human immortalized keratinocytes (HaCaT cell line). Antioxidant activity was assessed by six different assays, while beneficial potential against certain neurodegenerative diseases and type 2 diabetes was determined in selected enzyme inhibition assays coupled with molecular modeling. The results showed that the obtained extracts were rich in phenolic compounds, especially flavonoid glycosides such as rutin and kaempferol 3-O-glucoside. All of the extracts showed antimicrobial, wound-healing, antioxidant and anti-enzymatic properties. This study is the first of its kind, linking the medieval medicinal use of wild-growing flowers of A. scorodoprasum with contemporary in vitro scientific approaches.

Biochemical and Antioxidant Properties as well as Antimicrobial and Antibiofilm Activities of Allium scorodoprasum subsp. jajlae (Vved.) Stearn

“…jajlae in our study, as determined by the GC-MS analysis, identified linoleic acid, palmitic acid, and octadecanoic acid 2,3-dihydroxypropyl ester as the major compounds in the ethanol extract. In contrast, Ðordevski et al [ 44 ] found flavonoids, such as rutin and kaempferol 3-O-glucoside, to be the most abundant compounds in the extracts of A. scorodoprasum L. flowers.…”

Characterization, In Vitro Biological Activity and In Vivo Cardioprotective Properties of Trametes versicolor (L.:Fr.) Quél. Heteropolysaccharides in a Rat Model of Metabolic Syndrome

“…All the microoganisms were deposited at bacterial and fungal unit collection of the Mycological laboratory of the Institute for Biological Research “Siniša Stanković”, National Institute of the Republic of Serbia, University of Belgrade. Evaluated pathogenic microorganisms were clinical samples, isolated from the infected skin [ 49 ]. Antibacterial and anticandidal activities of TVH were evaluated using microdilution method in 96-well microtiter plates.…”

“…Antibacterial and anticandidal activities of TVH were evaluated using microdilution method in 96-well microtiter plates. The minimum inhibitory concentrations (MIC) and minimal bactericidal/fungicidal concentrations (MBC/MFC) of extracts were determined as described previously [ 49 ]. Streptomycin and ketoconazole were used as positive controls.…”

Solvent System-Guided Extraction of Centaurium spicatum (L.) Fritch Provides Optimized Conditions for the Biological and Chemical Characteristics of the Herbal Extracts

“…The cytotoxic effect of the extracts was determined using a spontaneously immortalized keratinocyte cell line (HaCaT, AddexBio T0020001) in the crystal violet assay, as previously described by Stojković et al [ 49 ] with some modifications. HaCaT cells were grown in high-glucose Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, Gibco, Life Technologies Ltd., U.K.), 2 mM L-glutamine, and 1% antibiotic-antimycotic (Gibco, Life Technologies Corporation, Carlsbad, CA, USA) at 37 °C in a 5% CO 2 incubator.…”

“…The assay was performed as described by Đorđevski et al [ 49 ] with some modifications. HaCaT cells were grown until reaching 85% confluence.…”