Chemical and biological oxidative effects of carbon black nanoparticles

Koike, Eiko; Kobayashi, Takuro

doi:10.1016/j.chemosphere.2006.03.078

Cited by 118 publications

(64 citation statements)

References 27 publications

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“…Nanoparticles can induce oxidative stress in alveolar epithelial cells due to their interactions with cellular components. Such interactions can induce oxidative stress, although not all nanomaterials with various electronic configurations and diverse surface properties will induce spontaneous generation of reactive oxygen species (ROS) [17,18]. Oxidative stress has been implicated in the pathogenesis of neurodegenerative diseases such as Parkinson's and Alzheimer's diseases [19].…”

Section: Discussionmentioning

confidence: 99%

Differential cytotoxicity of metal oxide nanoparticles

Chen

Zhu

Cho

et al. 2008

Journal of Experimental Nanoscience

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Concerns about the potential health hazards of nanomaterials are growing. To determine the potential toxicity of metal oxide nanoparticles, human SH-SY5Y neuroblastoma and H4 neuroglioma cells were exposed to ) at a concentration range of 0.01-100 mM for 48 h, under the cell culture conditions: 95% O 2 , 5% CO 2 , 85% humidity, 37C. Their ensemble cell viability was determined by MTS cell proliferation assay. A live/dead cell assay was also performed, and cellular images were acquired by a high-content fluorescence microscope and quantified by a novel computerised image analysis protocol. Our data indicated that exposure of these nanoparticles induced differential toxic effects in both SH-SY5Y and H4 cells, and the cells had dose-dependent toxic responses to the CuO nanoparticle insult. In conclusion, the toxic responses of the nanoparticles are complex, and they warrant further in vivo studies. However, it remains to be determined if these nanopartilces have synergistically enhancing or cancelling toxic effects upon both SH-SY5Y and H4 cells.

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Section: Discussionmentioning

confidence: 99%

Differential cytotoxicity of metal oxide nanoparticles

Chen

Zhu

Cho

et al. 2008

Journal of Experimental Nanoscience

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“…Previous studies have suggested particle size, transition metal levels and elemental carbon levels to be betPublished by Copernicus Publications on behalf of the European Geosciences Union. 4892 F. P. H. Wragg et al: An automated online instrument to quantify aerosol-bound ROS ter indicators than simple particle mass concentration (Godri et al, 2010;Kelly and Fussell, 2012;Koike and Kobayashi, 2006;Oberdorster et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

An automated online instrument to quantify aerosol-bound reactive oxygen species (ROS) for ambient measurement and health-relevant aerosol studies

Wragg¹,

Fuller²,

Freshwater³

et al. 2016

Atmos. Meas. Tech.

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Abstract. The adverse health effects associated with ambient aerosol particles have been well documented, but it is still unclear which aerosol properties are most important for their negative health impact. Some studies suggest the oxidative effects of particle-bound reactive oxygen species (ROS) are potential major contributors to the toxicity of particles. Traditional ROS measurement techniques are labour-intensive, give poor temporal resolution and generally have significant delays between aerosol sampling and ROS analysis. However, many oxidising particle components are reactive and thus potentially short-lived. Thus, a technique to quantify particle-bound ROS online would be beneficial to quantify also the short-lived ROS components.We introduce a new portable instrument to allow online, continuous measurement of particle-bound ROS using a chemical assay of 2 7 -dichlorofluorescein (DCFH) with horseradish peroxidase (HRP), via fluorescence spectroscopy. All components of the new instrument are attached to a containing shell, resulting in a compact system capable of automated continuous field deployment over many hours or days.From laboratory measurements, the instrument was found to have a detection limit of ∼ 4 nmol [H 2 O 2 ] equivalents per cubic metre (m 3 ) air, a dynamic range up to at least ∼ 2000 nmol [H 2 O 2 ] equivalents per m 3 air and a time resolution of ≤ 12 min. The instrument allows for ∼ 16 h automated measurement if unattended and shows a fast response to changes in concentrations of laboratory-generated oxidised organic aerosol. The instrument was deployed at an urban site in London, and particulate ROS levels of up to 24 nmol [H 2 O 2 ] equivalents per m 3 air were detected with PM 2.5 concentrations up to 28 µg m −3 .The new and portable Online Particle-bound ROS Instrument (OPROSI) allows fast-response quantification; this is important due to the potentially short-lived nature of particlebound ROS as well as fast-changing atmospheric conditions, especially in urban environments. The instrument design allows for automated operation and extended field operation with twice-daily presence of an operator. As well as having sensitivity suitable for ambient level measurement, the instrument is also suitable at concentrations such as those required for laboratory and chamber toxicological studies.

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“…In the assay, a buffered aqueous extract of a PM sample is mixed with DTT, which contains two electron-rich thiol groups. The catalytic capacity of DTT to transfer these electrons to molecular oxygen can be observed by measuring the DTT rate of decay, which has been correlated to oxidative stress markers in cellular systems (Li et al, 2003b;Koike and Kobayashi, 2006). Constituents of PM that have been shown as active redox cycling catalysts include black carbon from diesel particles (Shinyashiki et al, 2009) transition metals (Netto and Stadtman, 1996;Charrier and Anastasio, 2012), humic-like substances (Lin and Yu, 2011;Verma et al, 2012), and quinones (Kumagai et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Naphthalene SOA: redox activity and naphthoquinone gas–particle partitioning

2013

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Abstract. Chamber secondary organic aerosol (SOA) from low-NO x photooxidation of naphthalene by hydroxyl radical was examined with respect to its redox cycling behaviour using the dithiothreitol (DTT) assay. Naphthalene SOA was highly redox-active, consuming DTT at an average rate of 118 ± 14 pmol per minute per µg of SOA material. Measured particle-phase masses of the major previously identified redox active products, 1,2-and 1,4-naphthoquinone, accounted for only 21 ± 3 % of the observed redox cycling activity. The redox-active 5-hydroxy-1,4-naphthoquinone was identified as a new minor product of naphthalene oxidation, and including this species in redox activity predictions increased the predicted DTT reactivity to 30 ± 5 % of observations. These results suggest that there are substantial unidentified redox-active SOA constituents beyond the small quinones that may be important toxic components of these particles. A gas-to-SOA particle partitioning coefficient was calculated to be (7.0 ± 2.5) × 10 −4 m 3 µg −1 for 1,4-naphthoquinone at 25 • C. This value suggests that under typical warm conditions, 1,4-naphthoquinone is unlikely to contribute strongly to redox behaviour of ambient particles, although further work is needed to determine the potential impact under conditions such as low temperatures where partitioning to the particle is more favourable. Also, higher order oxidation products that likely account for a substantial fraction of the redox cycling capability of the naphthalene SOA are likely to partition much more strongly to the particle phase.

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Chemical and biological oxidative effects of carbon black nanoparticles

Cited by 118 publications

References 27 publications

Differential cytotoxicity of metal oxide nanoparticles

Differential cytotoxicity of metal oxide nanoparticles

An automated online instrument to quantify aerosol-bound reactive oxygen species (ROS) for ambient measurement and health-relevant aerosol studies

Naphthalene SOA: redox activity and naphthoquinone gas–particle partitioning

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