Chemical and electric transmission in the carotid body chemoreceptor complex

Eyzaguirre, C.

doi:10.4067/s0716-97602005000400005

Cited by 18 publications

(8 citation statements)

References 29 publications

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“…The subsequent question is what neurotransmitter(s) are responsible for translating the glomus cell activation into an electric signal in the afferent endings of the CSN. A remarkable feature of the CB is the presence of a broad diversity of endogenous neuroactive substances, including both the classical neurotransmitters and the neuromodulators (Eyzaguirre, 2005). It has been proposed that these substances play roles in the transmission of chemosensory information (Iturriaga & Alcayaga, 2004; Bairam & Carroll, 2005; Nurse, 2005).…”

Section: Discussionmentioning

confidence: 99%

IL‐1β inhibits I_K and increases [Ca²⁺]_i in the carotid body glomus cells and increases carotid sinus nerve firings in the rat

Shu

Wang

et al. 2007

Eur J of Neuroscience

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Increasing evidence indicates that there exists a reciprocal communication between the immune system and the brain. Interleukin 1beta (IL-1beta), a proinflammatory cytokine produced during immune challenge, is believed to be one of the mediators of immune-to-brain communication, but how it gets into the brain is unknown because of its large molecular weight and difficulty in crossing the blood-brain barrier. Our previous work has demonstrated that IL-1 receptor type I is strongly expressed in the glomus cells of rat carotid body (CB), a well characterized polymodal chemoreceptive organ which serves not only for the detection of hypoxia, hypercapnia and acidity, but also for low temperature and blood glucose. The present study was designed to test whether IL-1beta could stimulate the CB glomus cells and alter the discharge properties in the carotid sinus nerve, the afferent nerve innervating the organ. The results from whole-cell patch-clamp recordings and calcium imaging showed that extracellular application of IL-1beta significantly decreased the outward potassium current and triggered a transient rise in [Ca(2+)](i) in the cultured glomus cells of rat CB. Furthermore, by using extracellular recordings and pharmacological intervention, it was found that IL-1beta stimulation of the CB in the anaesthetized rat in vivo significantly increased the discharge rate in the carotid sinus nerve, most probably mediated by ATP release. This experiment provides evidence that the CB responds to cytokine stimulation and proposes the possibility that the CB might play a role in immune-to-brain communication.

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Section: Discussionmentioning

confidence: 99%

IL‐1β inhibits I_K and increases [Ca²⁺]_i in the carotid body glomus cells and increases carotid sinus nerve firings in the rat

Shu

Wang

et al. 2007

Eur J of Neuroscience

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“…In the case of enzymes, a process‐driven customisation is achievable through the combination of molecular evolution techniques and highly efficient expression systems . Furthermore, like the cellular post‐translational modifications that increase the functional diversity of the proteome (e.g., the covalent addition of functional groups, the formation of intra‐ or intermolecular bonds or a proteolytic cleavage), the functionality of recombinant enzymes can be refined using well‐established techniques of chemical modification of the enzyme surface . The literature is abundant with examples of proteins covalently modified with tags of all kinds for different purposes: purification, stabilisation, labelling, or even editing enzyme function .…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3] Furthermore,l ike the cellular post-translational modificationst hat increase the functional diversity of the proteome (e.g.,t he covalent addition of functional groups, the formation of intra-or intermolecular bonds or ap roteolytic cleavage), the functionality of recombinant enzymesc an be refined using well-established techniques of chemical modification of the enzymes urface. [4][5][6][7] The literature is abundant with examples of proteins covalently modified with tags of all kinds for different purposes:p urification, stabilisation, labelling, or even editing enzymef unction. [8][9][10][11] Therefore, the combination of surface-located modulators with robust enzymes offers opportunities for developing biomolecule-based catalysts with entirely new sets of functions.…”

Section: Introductionmentioning

confidence: 99%

Probing the Surface of a Laccase for Clues towards the Design of Chemo‐Enzymatic Catalysts

et al. 2017

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Systems featuring a multi-copper oxidase associated with transition-metal complexes can be used to perform oxidation reactions in mild conditions. Here, a strategy is presented for achieving a controlled orientation of a ruthenium–polypyridyl graft at the surface of a fungal laccase. Laccase variants are engineered with unique surface-accessible lysine residues. Distinct ruthenium–polypyridyl-modified laccases are obtained by the reductive alkylation of lysine residues precisely located relative to the T1 copper centre of the enzyme. In none of these hybrids does the presence of the graft compromise the catalytic efficiency of the enzyme on the substrate 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid). Furthermore, the efficiency of the hybrids in olefin oxidation coupled to the light-driven reduction of O2 is highly dependent on the location of the graft at the enzyme surface. Simulated RuII–CuII electron coupling values and distances fit well the observed reactivity and could be used to guide future hybrid designs.L.S. was the recipient of a MinistHre de l’Education Nationale fellowship.\ud This study was supported by grants from the Agence Nationale de la Recherche (ANR-09-BLANC-0176 and ANR-15-CE07-0021-01) and from the Ministerio de EconomÍa, Industria y\ud Competitividad (CTQ2016-79138-R). We thank Elise Courvoisier-Dezord from the Plateforme AVB (AMU): Analyse et Valorisation\ud de la Biodiversit8 and Yolande Charmasson for help in the production of the recombinant enzymes, as well as Pascal Mansuelle\ud and R8gine Lebrun from the Plateforme Prot8omique (CNRSAMU) for help in acquiring mass spectrometry data.Peer ReviewedPostprint (published version

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“… Simultaneous intracellular (sharp electrode) recordings of glomus cells and afferent nerve endings in a slice preparation of rodent carotid body failed to detect any excitatory coupling between the glomus cell and nerve ending – a result that Eyzaguirre characterized as “puzzling” (Eyzaguirre, 2005; Jiang and Eyzaguirre, 2006). …”

Section: Summary and Future Directionsmentioning

confidence: 99%

Voltage-gated Na+ channels in chemoreceptor afferent neurons—Potential roles and changes with development

Donnelly

2013

Respiratory Physiology & Neurobiology

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Carotid body chemoreceptors increase their action potential (AP) activity in response to a decrease in arterial oxygen tension and this response increases in the post-natal period. The initial transduction site is likely the glomus cell which responds to hypoxia with an increase in intracellular calcium and secretion of multiple neurotransmitters. Translation of this secretion to AP spiking levels is determined by the excitability of the afferent nerve terminals that is largely determined by the voltage-dependence of activation of Na+ channels. In this review, we examine the biophysical characteristics of Na+ channels present at the soma of chemoreceptor afferent neurons with the assumption that similar channels are present at nerve terminals. The voltage dependence of this current is consistent with a single Na+ channel isoform with activation around the resting potential and with about 60-70% of channels in the inactive state around the resting potential. Channel openings, due to transitions from inactive/open or closed/open states, may serve to amplify external depolarizing events or generate, by themselves, APs. Over the first two post-natal weeks, the Na+ channel activation voltage shifts to more negative potentials, thus enhancing the amplifying action of Na+ channels on depolarization events and increasing membrane noise generated by channel transitions. This may be a significant contributor to maturation of chemoreceptor activity in the post-natal period.

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Chemical and electric transmission in the carotid body chemoreceptor complex

Cited by 18 publications

References 29 publications

IL‐1β inhibits I_K and increases [Ca²⁺]_i in the carotid body glomus cells and increases carotid sinus nerve firings in the rat

IL‐1β inhibits I_K and increases [Ca²⁺]_i in the carotid body glomus cells and increases carotid sinus nerve firings in the rat

Probing the Surface of a Laccase for Clues towards the Design of Chemo‐Enzymatic Catalysts

Voltage-gated Na+ channels in chemoreceptor afferent neurons—Potential roles and changes with development

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Chemical and electric transmission in the carotid body chemoreceptor complex

Cited by 18 publications

References 29 publications

IL‐1β inhibits IK and increases [Ca2+]i in the carotid body glomus cells and increases carotid sinus nerve firings in the rat

IL‐1β inhibits IK and increases [Ca2+]i in the carotid body glomus cells and increases carotid sinus nerve firings in the rat

Probing the Surface of a Laccase for Clues towards the Design of Chemo‐Enzymatic Catalysts

Voltage-gated Na+ channels in chemoreceptor afferent neurons—Potential roles and changes with development

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Product

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About

IL‐1β inhibits I_K and increases [Ca²⁺]_i in the carotid body glomus cells and increases carotid sinus nerve firings in the rat

IL‐1β inhibits I_K and increases [Ca²⁺]_i in the carotid body glomus cells and increases carotid sinus nerve firings in the rat