Chemical and Genetic Differentiation of <i>Ligularia tsangchanensis</i> in Yunnan and Sichuan Provinces of China

Torihata, Atsushi; Hanai, Ryo; Gong, Xun; Shen, Yingbin; Kuroda, Chiaki

doi:10.1002/cbdv.200790042

Cited by 22 publications

(15 citation statements)

References 32 publications

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“…Cacalol has been isolated from various medicinal plants including Cacalia delphiniifolia, which is a popular edible wild plant in Asian countries [20,21]. Cacalol was also identified as major anti-hyperglycemic and anti-inflammatory sesquiterpene in the aqueous extract from the roots of Psacalium decompositum, which has been used as a traditional medicine in Mexico and the Southwestern United States for the treatment of rheumatism, fever, skin infections and ulcers, diabetes and tumors [22,23].…”

Section: Discussionmentioning

confidence: 99%

Cacalol, a natural sesquiterpene, induces apoptosis in breast cancer cells by modulating Akt-SREBP-FAS signaling pathway

Furuta

Shindo

et al. 2010

Breast Cancer Res Treat

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We previously isolated cacalol as a free radical-scavenging compound from Cacalia delphiniifolia which is a traditional Asian herbal plant and is believed to have medicinal effects on cancer. In this report, we demonstrated that cacalol has strong anti-proliferation effect on breast cancer cells and induces apoptosis by activating a pro-apoptotic pathway. We also found that a combination of cacalol and other chemotherapeutic drugs (Taxol and cyclophosphamide) synergistically induced apoptosis and partially overcame chemo-resistance. To further gain a mechanistic insight, we tested a potential inhibitory effect of cacalol on fatty acid synthase gene (FAS) in breast cancer cells, and found that cacalol significantly modulated the expression of the FAS gene, which resulted in apoptosis through activation of DAPK2 and caspase 3. We have also shown that cacalol significantly suppressed the Akt-sterol regulatory element-binding proteins (SREBP) signaling pathway and concomitant transcriptional activation of FAS. In a xenograft model of nude mouse, when cacalol was administered intraperitoneally, tumor growth was significantly suppressed. Importantly, oral administration of cacalol before implanting tumors showed significant preventive effect on tumor growth in the same animal model. Furthermore, the treatment of mice with a combination of low dose of Taxol and cacalol significantly suppressed the tumor growth. Taken together, our results indicate that cacalol induces apoptosis in breast cancer cells and impairs mammary tumor growth in vivo by blocking the expression of the FAS gene through modulation of Akt-SREBP pathway, suggesting that cacalol has potential utility as a chemopreventive and chemotherapeutic agent for breast cancer.

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Section: Discussionmentioning

confidence: 99%

Cacalol, a natural sesquiterpene, induces apoptosis in breast cancer cells by modulating Akt-SREBP-FAS signaling pathway

Furuta

Shindo

et al. 2010

Breast Cancer Res Treat

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“…Cacalol has been isolated from various medicinal plants including Chinese herbs Ligularia tsangchanensis and Roldana angulifolia as well as Psacalium. decompositum, which is widely used as a traditional medicine in Mexico and the Southwestern United States for the treatment of rheumatism, fever and ulcers [110][111][112][113][114]. A sesquiterpene derivative has also been patented recently which has anti-tumor effects [115].…”

Section: Cacalolmentioning

confidence: 99%

Anti-Cancer Drugs Targeting Fatty Acid Synthase (FAS)

Pandey¹,

Liu²,

Xing³

et al. 2012

PRA

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Fatty acid synthase (FAS) is a key enzyme of the fatty acid biosynthetic pathway which catalyzes de novo lipid synthesis. FAS expression in normal adult tissues is generally very low or undetectable as majority of fatty acids obtained are from dietary sources, whereas it is significantly upregulated in cancer cells despite adequate nutritional lipid supply. Activation of FAS provides rapidly proliferating tumor cells sufficient amount of lipids for membrane biogenesis and confers growth and survival advantage possibly acting as a metabolic oncogene. Importantly, inhibition of FAS in cancer cells using the pharmacological FAS inhibitors results in tumor cell death by apoptosis whereas normal cells are resistant. Due to this differential expression of FAS, the inhibitors of this enzyme are selectively toxic to tumor cells and therefore FAS is considered an attractive therapeutic target for cancer. Several FAS inhibitors are already patented and commercially available; however, the potential toxicity of these FAS inhibitors remains to be tested in clinical trials. In this review, we discuss some of the potent FAS inhibitors along with their patent information, the mechanism of anti-cancer effects and the development of more specific and potent FAS inhibitors with lower side effects that are expected to emerge as anti-cancer treatment in the near future.

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“…(Asteraceae) in the Hengduan Mountains area provides interesting samples for the study of plant diversity, since the genus is highly diversified and their genetic differentiation is considered to be still continuing in the area. The chemical and genetic diversity of some Ligularia species has been investigated by chemical constituents and DNA sequences for exploring its speciation and evolution (Nagano et al 2007(Nagano et al , 2006Kuroda et al 2007;Torihata et al 2007;Hanai et al 2005). In order to devise adequate genetic diversification, conservation, and management strategies for Ligularia species, we developed microsatellite markers (SSR) from Ligularia hodgsonii using the Fast Isolation by AFLP of Sequences Containing repeats protocol (FIASCO) (Zane et al 2002); at the same time, no nuclear microsatellite primers have been reported for Ligularia.…”

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confidence: 99%

Isolation and characterization of microsatellite markers for Ligularia hodgsonii Hook. (Asteraceae)

et al. 2009

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Ligularia hodgsonii is one of widely distributed Ligularia species in south China, central China, Far East area of Russia and Japan. In this study, we described the development of 14 microsatellite markers from the genome of L. hodgsonii using the FIASCO protocol. Polymorphism of each locus was assessed in 30 adult individuals of the Ligularia. The average allele number of the microsatellites was 3.0 per locus, ranging from 2 to 5. The observed (H O ) and expected (H E ) heterozygosities varied from 0.2000 to 0.7333 and from 0.3910 to 0.7598. The marker transferability of the 14 primer pairs was tested on other two congeneric species that also occur in China.

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Chemical and Genetic Differentiation of Ligularia tsangchanensis in Yunnan and Sichuan Provinces of China

Cited by 22 publications

References 32 publications

Cacalol, a natural sesquiterpene, induces apoptosis in breast cancer cells by modulating Akt-SREBP-FAS signaling pathway

Cacalol, a natural sesquiterpene, induces apoptosis in breast cancer cells by modulating Akt-SREBP-FAS signaling pathway

Anti-Cancer Drugs Targeting Fatty Acid Synthase (FAS)

Isolation and characterization of microsatellite markers for Ligularia hodgsonii Hook. (Asteraceae)

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