Chemical and histochemical characterization of mucopolysaccharides in a jaw myxoma

Hodson, J. J.; Prout, R.E.S.

doi:10.1136/jcp.21.5.582

Cited by 33 publications

(18 citation statements)

References 27 publications

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“…In two of the myxomas in the present investigation, in the umbilical cords, and in the myxoma studied by Hodson and Prout (1968) incubation in saline alone appeared to cause a loss of acidic mucosubstance from the sections. One feature common to all these tissues appears to be the presence of hyaluronic acid, and possibly some of this is lost during the saline incubation.…”

Section: Discussionsupporting

confidence: 37%

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Odontogenic myxoma: Ultrastructural and histochemical studies

Harrison

1973

J Clin Pathol

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SYNOPSIS An odontogenic myxoma of a maxilla has been examined. Histochemistry of the mucosubstance indicated that hyaluronic acid and chondroitin sulphate were present. On ultrastructural examination many ofthe cells in the myxomatous tissue were seen with prominent rough endoplasmic reticulum, suggesting a secretory function, and possibly the myxomatous ground substance was produced by these cells. Cells containing collagen fibrils were found. Epithelial islands with intercellular spaces producing an arrangement similar to the stellate reticulum of the enamel organ were found, and possibly had developed from odontogenic epithelial rests. These islands were surrounded by a clear zone, outside which were cells with an increased prominence of adenosine-triphosphatase, nucleoside-diphosphatase, thiamine-pyrophosphatase, and often arylamidase reaction products. This probably represents a reaction of the mesodermal tissue to the epithelial islands. Possibly the epithelium exerts an inductive effect on the mesodermal tissue, and the myxomatous appearance may be the result of an aberrant development of mesodermal cells into 'myxoblasts' which secrete the myxomatous ground substance.A considerable number of odontogenic myxomas have been studied by means of routine histological techniques (Zimmerman and Dahlin, 1958;Barros, Dominguez, and Cabrini, 1969;Gorlin, 1970;Pindborg, 1970;Whitman, Stewart, Stoopack, and Jerrold, 1971;Cawson, 1972). Two more specialized studies have been a thorough study of the chemistry and histochemistry of the mucosubstance by Hodson and Prout (1968) and a histochemical study by Sedano and Gorlin (1965), both these studies being primarily of single cases.In the present investigation a maxillary tumour which was found on preliminary biopsy to be an odontogenic myxoma has been studied after removal by means of routine histological techniques, enzyme and mucosubstance histochemistry, and electron microscopy. Materials and MethodsThe tumour occupied the right maxilla of an otherwise fit woman of 21 years who had noticed swelling of the right face for at least three years. The case history will be reported elsewhere (Harrison and Eggleston, 1973). Upon removal of the tumour a Received for publication 31 May 1973. pyramid of tissue extending to the centre of the tumour was removed. The cut surface was white and the tissue was of a rubbery consistency. The apical part of this pyramid was preserved in several ways. MUCOSUBSTANCE HISTOCHEMISTRY AND ROUTINE HISTOLOGYA piece of tissue was fixed overnight at 04' in 10% formalin with 09 9% saline and 2% calcium acetate and was subsequently wax-embedded. Sections were cut at 5 ,u.Mucosubstances containing vic-glycols were demonstrated by the periodic-acid Schiff technique (Stoward, 1967, but with Schiff's reagent from G. T. Gurr). Glycogen was detected by the disappearance of its staining with the periodic-acid Schiff technique after incubating sections for one hour at 370 in an aqueous solution of 0-1 % malt diastase (Sigma).Acidic mucosubstances were de...

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Section: Discussionsupporting

confidence: 37%

“…Two more specialized studies have been a thorough study of the chemistry and histochemistry of the mucosubstance by Hodson and Prout (1968) and a histochemical study by Sedano and Gorlin (1965), both these studies being primarily of single cases.…”

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confidence: 99%

Odontogenic myxoma: Ultrastructural and histochemical studies

Harrison

1973

J Clin Pathol

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“…24 Radiographically, odontogenic myxomas present as unilocular or multilocular radiolucent lesions sometimes described as having a "soap bubble", "honeycomb", "mottled", "cob web" or "tennis racket" appearance with fine bony trabeculae. 29,30 Occasional binucleated cells, intense cytoplasmic eosinophilia, mild pleomorphism, as well as a few mitotic figures may also be seen in these lesions 31 but lesions with necrosis, and abundant and abnormal mitotic structures should be evaluated carefully to exclude myxoid malignancies like a lowgrade myxoid fibrosarcoma. Bony expansion of the affected bone, root resorption, and tooth displacement may be visualized on radiographic examination.…”

Section: Discussionmentioning

confidence: 99%

Diagnosis and Management of Odontogenic Myxoma in a Dog

2007

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A three-year-old Jack Russell terrier dog was presented with a large gingival mass of the right mandible extending from the fourth premolar to the first molar teeth. Radiographic examination of the expansile mass revealed moth-eaten, honeycomb-like lyses of the mandible and extended into the mandibular alveolar canal based on computed tomography. The histopathological diagnosis of the biopsy was odontogenic fibromyxoma. Mandibulectomy with resection of the associated soft tissues was performed. Surgical management was curative with no clinical signs of disease 2-years after treatment. These neoplasms are slow growing, locally destructive tumors of odontogenic origin that have been described in the jaw of only one dog. In this paper, the clinico-radiological and pathologic features, diagnostic modalities as well as the factors that might influence treatment outcome of odontogenic myxomas are discussed. These odontogenic tumors are currently excluded from the WHO classification of odontogenic tumors in domestic animals and inclusion in future classifications systems is proposed.

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“…All sections of tumours were stained with haematoxylin and eosin (HE) together with the following stains for mucin (table I): periodic acid Schiff (PAS) (McManus, 1946) with and without diastase; Alcian Blue and PAS (AB/PAS) (Mowry, 1956); aldehyde fuchsin and AB (AF/AB) (Spicer and Myer, 1960); Alcian Yellow and AB (AY/AB) (Ravetto, 1964); phenylhydrazine-PAS (Phe PAS) (Spicer, 1961); Alcian Blue methylated (AB-meth) (Fisher and Lillie, 1954) when the mild methylation procedure was used; Alcian Bluemethylated followed by saponification (AB-meth-sap) (Spicer and Lillie, 1959) when the strong methylation procedure was employed followed by saponification with potassium hydroxide; metachromatic stains (Hodson and Prout, 1968); Azure A was employed at pH 1-5 and sulphated mucins were metachromatically stained at this pH; high iron diamine method (HID) (Lev and Spicer, 1965) in which sections were exposed for 16 hr to the diamine mixture. SialidaseAB/PAS (Spicer and Warren, 1960) for which sialidase enzyme from Vibrio cholerae, 500 units per ml at pH 5*5, was obtained from Koch Light Laboratories.…”

Section: Histochemical Studiesmentioning

confidence: 99%

Mucin histochemistry of mucous carcinomas of breast and colon and non-neoplastic breast epithelium

Cooper

1974

J Clin Pathol

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Although mucous carcinomas of the breast represent only 2 % of all mammary carcinomas, these were the first of the histological types to be recognized. Otto in 1816 (quoted by Gaabe, 1908) was probably the first to describe it. These tumours have been credited with a more favourable prognosis (Gaabe, 1908;Geschickter, 1938;Silverberg, Kay, Chitale, and Levitt, 1971).Although it has long been established that mucous carcinomas of the gastrointestinal tract secrete epithelial mucin (Stinson, 1928), there has been controversy as to the type of the mucin secreted by mammary mucous carcinomas, and it is not widely recognized that mucin is secreted by non-neoplastic epithelial tissue. There is also controversy as to the exact site of origin of the mucous carcinomas of the breast.The purpose of this study is to confirm the epithelial origin of the mucin produced by mucous carcinoma of the breast, using mucous carcinomas of the colon as a comparison, and to demonstrate that epithelial mucin is produced by non-malignant breast epithelia using sections of normal breast and 'Present address.

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Chemical and histochemical characterization of mucopolysaccharides in a jaw myxoma

Cited by 33 publications

References 27 publications

Odontogenic myxoma: Ultrastructural and histochemical studies

Odontogenic myxoma: Ultrastructural and histochemical studies

Diagnosis and Management of Odontogenic Myxoma in a Dog

Mucin histochemistry of mucous carcinomas of breast and colon and non-neoplastic breast epithelium

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