Chemical and Physical Properties of Lipopolysaccharide of
            <i>Yersinia pestis</i>

Hartley, James L.; Adams, G. A.; Tornabene, T. G.

doi:10.1128/jb.118.3.848-854.1974

Cited by 40 publications

(18 citation statements)

References 36 publications

(32 reference statements)

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“…The KDO levels were 0.46 and 0.57% of the LPS (C-M) and (H-Ph). Although these values are less than those found in the LPS of many gram-negative bacteria (18,25), they resemble those described for Fusobacterium necrophorum (17) and Neisseria gonorrhoeae (29) type T4. No sialic acid was detectable.…”

Section: Resultssupporting

confidence: 54%

“…The major components are isomyristic, palmitic, and ,/-hydroxymyristic acids. The LPS contained 7% fatty acids, and, although the amount is similar to that of Yersinia pestis (18), it is well known that the fatty acids of the LPS of different species and genera vary (24). There were large relative amounts of other fatty acids (Table 4), 2.9 C14 (myristic) 1.1 anteiso-C15 (sarcinic) 33.1 iso-C18 (isopalmitic) 3.4 C1i (palmitic) 9.9 anteiso-C,7 (14-methyl hexadecanoic) 34 notably, myristic, stearic, and pentadecanoic acids.…”

Section: Resultsmentioning

confidence: 91%

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Further characterization of a lipopolysaccharide from Coxiella burneti

Chan¹,

McChesney²,

Paretsky³

1976

Infect Immun

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The lipopolysaccharide previously isolated from the rickettsial agent of Q fever, Coxiella burneti, phase I, has been further characterized. The sugar residues ribose, mannose, glucose, D-glycero-D-mannoheptose, and L-glycero-Dmannoheptose are present. Two sugars remain unidentified, one of which is a minor and the other a major constituent. Isomyristic, palmitic, and /3-hydroxymyristic acids are the major fatty acid residues of the 15 identified. The nature and content of other lipopolysaccharide constituents are presented.Toxins have been identified in several rickettsial species, but until recently no endotoxin had been described for Coxiella burneti, the rickettsial agent of Q fever. Baca and Paretsky prepared a lipopolysaccharide (LPS) from C. burneti, phase I, which has many pharmacological properties characteristic of the endotoxins of gram-negative bacteria (3). We now describe a more extensive chemical characterization of the LPS of C. burneti.MATERIALS AND METHODS C. burneti, Nine Mile strain, phase I, was cultivated in embryonated eggs and subsequently purified from infected yolk sacs (33). The LPS was prepared by a modified hot phenol-water method (4, 36). Purified rickettsiae (2 to 4 g, wet weight) were suspended in 20 ml of 0.02 M phosphate buffer-0.15 M KCl, pH 7.4 (PK), and 20 ml of 90% redistilled phenol (wt/wt), 68 C, was immediately added. The suspension was vigorously shaken for 20 min in a water bath at 68 C. The emulsion was centrifuged at 3,000 x g for 15 min, and the aqueous phase was collected. The residual interphase and phenolic phase were extracted twice more with an equal volume of PK, and the aqueous phases were collected, pooled, and dialyzed against running tap water for 3 days. The residual opalescent solution was centrifuged at 10,000 x g for 20 min at 4 C. To the supernatant were added enough 0.5 M tris(hydroxymethyl)aminomethane buffer and 0.5 M MgCl2 to give a final concentration of 0.025 M tris(hydroxymethyl)aminomethane, pH 7.5, and 0.01 M MgCl2, together with 100 gg of ribonuclease (Sigma type

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Section: Resultssupporting

confidence: 54%

Section: Resultsmentioning

confidence: 91%

Further characterization of a lipopolysaccharide from Coxiella burneti

Chan¹,

McChesney²,

Paretsky³

1976

Infect Immun

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“…In the Y. pestis lipid A spectrum, the major peak at m/z 1824 was followed by a minor one at m/z 1840, suggesting that a small portion of the hexaacyl lipid A has an additional hydroxyl group. Any differences between the quantities of non‐polar fatty acids (including C 16:1 ) found by us and those found by others [8,22,23] are most likely due to different analytical methods, a difference in strain (in one case), differences in culture conditions, or simply the kind of variability that can occur in different preparations obtained under identical conditions. It should be noted that when the molecular ion at m/z 1770 is dominant in the other Yersinia , there is also an usually small m/z 1798 peak.…”

Section: Discussionmentioning

confidence: 51%

Novel variation of lipid A structures in strains of different Yersinia species¹

et al. 1999

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The Yersinia genus includes human and animal pathogens (plague, enterocolitis). The fine structures of the endotoxin lipids A of seven strains of Yersinia enterocolitica, Yersinia ruckeri and Yersinia pestis were determined and compared using mass spectrometry. These lipids differed in secondary acylation at C-2P P: this was dodecanoic acid (C 12 ) for two strains of Y. enterocolitica and Y. ruckeri, tetradecanoic acid (C 14 ) in two other Y. enterocolitica and hexadecenoic acid (C 16:1 ) in Y. pestis. The enterocolitica lipids having a mass identical to that of Escherichia coli were found to be structurally different. The results supported the idea of a relation between membrane fluidity and environmental adaptability in Yersinia.z 2000 Federation of European Biochemical Societies.

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“…In contrast to Y. pseudotuberculosis, strains of Y. pestis are non-motile, do not produce urease and do not produce acid from rhamnose and melibiose, and identifying strains of Y. pestis based on a series of negative attributes is unsatisfactory. The report that strains of Y. pestis do not produce long-chain lipopolysaccharide (LPS) (Hartley et al 1974;Darveau et al 1983) initiated the present study to examine the expression of LPS by strains of Y. pestis and Y. pseudotuberculosts, with the aim of developing a method for discriminating between these two species of Yersinia.…”

Section: Introductionmentioning

confidence: 99%