“…While free COU6 concentrated in the cytoplasm after 4 h incubation. This may be due to the high viscosity of AAP 38 , which causes it to tightly adhere to the cell membrane and prolongs its retention by targeted substrates, resulting in an increased drug accumulation in tumor cells. Meanwhile, the nanostructure of micelles also helps to increase the cell uptake.Figure 5Confocal microscopy images of MCF-7 cells incubated with three different concentrations of COU6 for 0.5 h, 1 h, 2 h or 4 h, blue: fluorescence of DAPI, green: fluorescence of COU6.
…”
The study was aimed to design a novel pH-sensitive carrier to deliver antitumor drugs to increase treatment efficiency. Histidine (His)was used to modify auricularia auricular polysaccharide (AAP) by esterification. Proton nuclear magnetic resonance spectrometry was developed to characterize the His-AAP carrier and the His-AAP Paclitaxel (PTX) micelles were prepared by self-assembled organic solvent evaporation. The formation of His-AAP PTX micelles was confirmed by dynamic light-scattering, transmission electron microscopy and high performance liquid chromatography. It was found that the His-AAP PTX micelles possessed a spherical morphology with an average diameter of 157.2 nm and an 80.3% PTX encapsulation efficiency. In vitro release at pH 7.4, 6.5, 5.0 reached 70%, 71%, and 88%, respectively. The cell viability assay and confocal laser scanning microscope were used to evaluate the cytotoxicity and cell uptake of the His-AAP PTX micelles. Compared with Taxol, the IC50 of the His-AAP PTX micelles were lower after incubating for 24 h, 48 h, or 72 h (0.216 versus 0.199, 0.065 versus 0.060, and 0.023 versus 0.005, respectively). In a test of tumor-bearing mice, the His-AAP PTX micelles significantly inhibited tumor growth. These results showed that His-AAP PTX micelles are a highly promising therapeutic system for anticancer therapy.
“…While free COU6 concentrated in the cytoplasm after 4 h incubation. This may be due to the high viscosity of AAP 38 , which causes it to tightly adhere to the cell membrane and prolongs its retention by targeted substrates, resulting in an increased drug accumulation in tumor cells. Meanwhile, the nanostructure of micelles also helps to increase the cell uptake.Figure 5Confocal microscopy images of MCF-7 cells incubated with three different concentrations of COU6 for 0.5 h, 1 h, 2 h or 4 h, blue: fluorescence of DAPI, green: fluorescence of COU6.
…”
The study was aimed to design a novel pH-sensitive carrier to deliver antitumor drugs to increase treatment efficiency. Histidine (His)was used to modify auricularia auricular polysaccharide (AAP) by esterification. Proton nuclear magnetic resonance spectrometry was developed to characterize the His-AAP carrier and the His-AAP Paclitaxel (PTX) micelles were prepared by self-assembled organic solvent evaporation. The formation of His-AAP PTX micelles was confirmed by dynamic light-scattering, transmission electron microscopy and high performance liquid chromatography. It was found that the His-AAP PTX micelles possessed a spherical morphology with an average diameter of 157.2 nm and an 80.3% PTX encapsulation efficiency. In vitro release at pH 7.4, 6.5, 5.0 reached 70%, 71%, and 88%, respectively. The cell viability assay and confocal laser scanning microscope were used to evaluate the cytotoxicity and cell uptake of the His-AAP PTX micelles. Compared with Taxol, the IC50 of the His-AAP PTX micelles were lower after incubating for 24 h, 48 h, or 72 h (0.216 versus 0.199, 0.065 versus 0.060, and 0.023 versus 0.005, respectively). In a test of tumor-bearing mice, the His-AAP PTX micelles significantly inhibited tumor growth. These results showed that His-AAP PTX micelles are a highly promising therapeutic system for anticancer therapy.
“…6)-D-glucosyl residues for every 3 main chain glucose residues, showing a comb-branched structure [12]. Previously, we also found that AP exhibited high viscosity, strong shear-thinning characteristics, and excellent thermal stability, indicating its great potential for applications in the food and pharmaceutical industries [13]. Starch is one of the most important food components in human diets and contributes to approximately 60-70% of the total energy consumed [14].…”
Section: Introductionmentioning
confidence: 87%
“…Therefore, the objective of this study was to systematically investigate the effects of the AP-blending ratio, temperature, pH, and ionic strength on the rheological properties of YS/AP combinations, based on our previous research on AP [13]. Both steady and dynamic shear rheological experiments were performed to characterize the rheological interactions, while scanning electron microscopy was used to evaluate the microstructures of composite gels.…”
Polysaccharides from - (AP) have unique functionalities. The influence of AP-blending ratios (0.1-0.8%), temperature, pH, and ionic strength on the rheological interactions of yam starch (YS)-AP blends (YA, 6%) was investigated. YA gels showed shear-thinning behavior with flow-behavior indices of 0.28-0.37 and greater pseudoplasticity in the presence of 0.8% AP. With incremental AP addition, the viscosity and consistency indices significantly increased, whereas the activation energy decreased from 7.55 to 5.12 kJ/mol ( < 0.05). YA gels exhibited excellent thickening and heat tolerance and behaved as weak gels over an AP concentration range of 0.1-0.4% but as true gels at higher concentrations (e.g. 0.8%). The viscosity/elasticity of the combinations weakened with NaCl addition or extreme pH values. A complex 3-dimensional network structure of the YA system was confirmed by scanning electron microscopy. These results indicated that AP has great potential for functional applications in the starchy food industry.
“…Resultados con un comportamiento similar se han encontrado anteriormente en los estudios de pulpa de ahuyama (Cucurbita moschata) (Quintana, Machacon, Marsiglia, Torregrosa & Garcia-Zapateiro, 2018), pulpa de papaya (Carica papaya) (Quintana et al, 2017) y de hongo comestible oreja de judas (Auricularia auricula-judae) (Honghui et al, 2016) y en purés de mango, papaya y melocotón (Guerrero & Alzamora, 1998).…”
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