Chemical and structural characterisation of iron cores of haemosiderins isolated from different sources

Ward, Roberta J.; Dickson, D.P.E.; Florence, Anne; Crichton, Robert R.; Mann, Stephen

doi:10.1111/j.1432-1033.1992.tb17356.x

Cited by 21 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…) . Iron‐dependent copper accumulation in hemosiderins and larger inclusions is consistent with the biochemical analysis of isolated hemosiderins, whereby the copper content was found to be the highest in hemosiderins obtained from patients with untreated HH . Cuproenzymes may be iron‐dependently induced to stabilize iron metabolism in iron‐rich cells.…”

Section: X‐ray Probe Microanalyses Of the Livers Of Patients With Gensupporting

confidence: 83%

The interactions between iron and copper in genetic iron overload syndromes and primary copper toxicoses in Japan

Tatsumi

Kato

et al. 2018

Hepatology Research

View full text Add to dashboard Cite

Iron and copper are trace elements essential for health, and iron metabolism is tightly regulated by cuproproteins. Clarification of the interactions between iron and copper may provide a better understanding of the pathophysiology and treatment strategy for hemochromatosis, Wilson disease, and related disorders. The hepcidin/ferroportin system was used to classify genetic iron overload syndromes in Japan, and ceruloplasmin and ATP7B were introduced for subtyping Wilson disease into the severe hepatic and classical forms. Interactions between iron and copper were reviewed in these genetic diseases. Iron overload syndromes were classified into pre-hepatic iron loading anemia and aceruloplasminemia, hepatic hemochromatosis, and post-hepatic ferroportin disease. The ATP7B-classical form with hypoceruloplasminemia has primary hepatopathy and late extra-hepatic complications, while the severe hepatic form is free from ATP7B mutation and hypoceruloplasminemia, and silently progresses to liver failure. A large amount of iron and trace copper co-exist in hepatocellular dense bodies of all iron overload syndromes. Cuproprotein induction to stabilize excess iron should be differentiated from copper retention in Wilson disease. The classical form of Wilson disease associated with suppressed hepacidin25 secretion may be double-loaded with copper and iron, and transformed to an iron disease after long-term copper chelation. Iron disease may not be complicated with the severe hepatic form with normal ferroxidase activity. Hepatocellular dense bodies of iron overload syndromes may be loaded with a large amount of iron and trace copper, while the classical Wilson disease may be double-loaded with copper and iron.

show abstract

Section: X‐ray Probe Microanalyses Of the Livers Of Patients With Gensupporting

confidence: 83%

The interactions between iron and copper in genetic iron overload syndromes and primary copper toxicoses in Japan

Tatsumi

Kato

et al. 2018

Hepatology Research

View full text Add to dashboard Cite

show abstract

“…We know that ferritin itself is degraded in mammalian cells to haemosiderin within the lysosomal compartment [39,40], which has an acidic pH of around 5, and that a continuum exists between ferritin, another ironcontaining protein designated prehaemosiderin and haemosiderin [40].…”

Section: Discussionmentioning

confidence: 99%

Optical and EPR spectroscopic studies of demetallation of hemin by L-chain apoferritins

Carette

Hagen

Bertrand

et al. 2006

Journal of Inorganic Biochemistry

Self Cite

View full text Add to dashboard Cite

“…In liver cells, ferritin molecules are organized either as individual cytosolic particles, or as clusters of variable size and shape, or they are found in complexes surrounded by a membrane (lysosomes). A process of digestion is believed to take place in these organelles, since biochemically water-insoluble iron components are found (haemosiderin) with some evidence of ferritin being the origin (O'Connell et al, 1986;Ward et al, 1992). The constitution of haemosiderin is ill defined, but it is known to have a high iron content.…”

Section: Characterization Of Iron Componentsmentioning

confidence: 99%

Quantitative electron spectroscopic imaging in bio‐medicine: evaluation and application

Beckers¹,

Gelsema²,

Bruijn³

et al. 1996

Journal of Microscopy

View full text Add to dashboard Cite

Electron spectroscopic imaging (ESI) with the energy-filtering transmission electron microscope enables the investigation of chemical elements in ultrathin biological sections. An analysis technique has been developed to calculate elemental maps and quantitative distributions from ESI sequences. Extensive experience has been obtained with a practical implementation of this technique. A procedure for more robust element detection has been investigated and optimized. With the use of Fe-loaded Chelex beads, the measurement system has been evaluated with respect to the linearity of the element concentration scale, the reproducibility of the measurements and the visual usage of image results. In liver specimens of a patient with an iron storage disease the detectability of iron was tested and we tried to characterize iron-containing components. The concentration measurement scale is approximately linear up to a relative section thickness of approximately equal to 0.5. Monitoring of this parameter is therefore considered to be important. The reproducibility was measured in an experiment with Fe-Chelex. The iron concentration differed by 6.4% between two serial measurements. Element distributions are in many applications interpreted visually. For this purpose the frequently used net-intensity distributions are regarded as unsuitable. For the quantification and visual interpretation of concentration differences mass thickness correction has to be performed. By contrast, for the detection of elements the signal-to-noise ratio is the appropriate criterion. Application of ESI analysis demonstrated the quantitative chemical capabilities of this technique in the investigation of iron storage diseases. Based on an assumed ferritin iron loading in vivo, different iron components can be discerned in liver parenchymal cells of an iron-overloaded patient.

show abstract

Chemical and structural characterisation of iron cores of haemosiderins isolated from different sources

Cited by 21 publications

References 15 publications

The interactions between iron and copper in genetic iron overload syndromes and primary copper toxicoses in Japan

The interactions between iron and copper in genetic iron overload syndromes and primary copper toxicoses in Japan

Optical and EPR spectroscopic studies of demetallation of hemin by L-chain apoferritins

Quantitative electron spectroscopic imaging in bio‐medicine: evaluation and application

Contact Info

Product

Resources

About