Late-glacial lake sediments containing the Laacher See Tephra (LST, 11 000 yr BP) have been analysed for pollen and diatoms at two sites (Hirschenmoor, Rotmeer) in the montane belt of the Black Forest (southern Germany) in order to detect the possible impact of this major volcanic eruption on terrestrial and aquatic biota.The pollen assemblages at both sites show a minor increase in grasses and sedges following the tephra deposition, whereas the pollen of aquatic macrophytes do not show any consistent pattern. Partial redundancy analysis and Monte Carlo permutation tests suggest, however, that Journal of Quaternary Science the LST had no statistically significant effect either on the terrestrial or aquatic pollen assemblages at either site when the effects of time and climatic change are allowed for statistically.The diatom assemblages at both sites changed after the deposition of the LST and diatominferred pH estimates suggest a short but non-significant excursion towards lower values. Multivariate analyses of the diatom data indicate a statistically significant impact of the deposition of the LST and associated changes in sediment lithology on the diatom assemblages when the effects of climatic change and time are allowed for statistically. However, owing to the interaction between tephra and lithology it is not possible to conclude if the diatom changes were caused by the deposition of the LST or by lithological changes.