Chemical characterization and antifungal activity of essential oil of capitula from wild Indian Tagetes patula L.

Abstract

The essential oil extracted by steam distillation from the capitula of Indian Tagetes patula, Asteraceae, was evaluated for its antifungal properties and analyzed by gas chromatography and gas chromatography-mass spectrometry. Thirty compounds were identified, representing 89.1% of the total detected. The main components were piperitone (24.74%), piperitenone (22.93%), terpinolene (7.8%), dihydro tagetone (4.91%), cis-tagetone (4.62%), limonene (4.52%), and allo-ocimene (3.66%). The oil exerted a good antifungal activity against two phytopathogenic fungi, Botrytis cinerea and Penicillium digitatum, providing complete growth inhibition at 10 microl/ml and 1.25 microl/ml, respectively. The contribution of the two main compounds, piperitone and piperitenone, to the antifungal efficacy was also evaluated and ultrastructural modifications in mycelia were observed via electron microscopy, evidencing large alterations in hyphal morphology and a multisite mechanism of action.

Antifungal activity of thyme oil againstGeotrichum citri-aurantii in vitroandin vivo

“…This was probably different from fungi treated by essential oils. Large alterations in hyphal morphology, abnormal branching of hypha in the apical region and loss of linearity with the appearance of barrel-like formations were observed in B. cinerea via electron microscopy under the effect of 1AE85 lg ml )1 of Tagetes patula L. essential oil (Romagnoli et al 2005). In our study, G. citri-aurantii treated with thyme oil showed alteration in the morphology, which appeared collapsed in mycelium and arthroconidia structure.…”

Sesquiterpene Rich Volatile Seed Oil of Tagetes Patula L. From Northwest Iran

“…Considering the mono and sesquiterpenoidal profile of the studied volatile oil, there was significant qualitative and quantitative differences between the data from the present experiment and reports of other scientists from elsewhere (Tables 1, 2 and3) [4,11,12,[14][15][16]. Isoaromadendrene epoxide (1.2%) was a newly identified compound from this plant with notable amounts compared with most of the mono and sesquiterpene components (Table 2).…”

“…Identification of components in the volatile oil was based on the comparison of their mass spectra and retention time with those of the authentic compounds and by computer matching with NIST and WILEY library as well as by comparison of the fragmentation pattern of the mass spectral data with those reported in the literature [1,4,11,12,[14][15][16] RESULTS AND DISCUSSION Tables 2 & 3 respectively. In total, forty components were identified in the seed essential oil of T. patula L. comprising 94% of the total oil (Tables 2 and 3).…”

“…For therapeutic purposes, this plant and its preparations have been used as astringent, flatulent, diuretic, sedative, stomachic and to treat colics, diarrhea, vomit, fever, skin diseases and hepatic disorders [12,13,17]. Furthermore, divergent biological activities have been devoted to the active principles of this plant as antibacterial, antifungal, insecticidal, nematicidal and larvicidal [2,3,8,11,12,14]. Essential oils extracted from the above ground parts and roots as well as acetylenic thiophenes derived from roots are the most important secondary metabolites of French marigold [14].…”

“…α-Terthienyl rich root secretions of French marigold have shown a suppressive effect on the population of soil-born nematodes [8]. There is considerable research interest toward the compositional analysis of T. patula L. volatile oil and its organic solvent extracts [4,11,12,14,15,16]. Literature survey revealed that there is several reports concerning the profiling of volatile oil of different natural and in-vitro culture derived organs of Tagetes patula L. with their main constituents summarized in Table1…”