Chemical characterization and biological abilities of Anthocleista djalonensis collected from two locations of Ivory Coast

Abstract

In this study, the total phenolic and flavonoid contents, HPLC‐DAD detected phytochemicals, antioxidant and enzyme inhibitory potential of methanolic and aqueous (as infusion) extracts of the medicinal plant, Anthocleista djalonensis (leaf and stem bark) collected from two locations, Mafiblé and Prikro, in Ivory Coast, were investigated. The ranges of total phenolic and flavonoid contents obtained were 14.17–46.95 mg gallic acid equivalent (GAE)/g and 2.96–34.76 mg rutin equivalent (RE)/g, respectively. Antioxidant abilities in terms of radical scavenging, reducing and metal chelating activity of the extracts in different assays were as follows: DPPH (4.90–48.82 mg trolox equivalent [TE]/g), ABTS (21.05–81.89 mg TE/g), CUPRAC (29.54–122.33 mg TE/g), FRAP (17.53–94.06 mg TE/g) and metal chelating (10.09–28.49 mg EDTAE/g. The extracts of A. djalonensis collected from Mafiblé, especially those of stem bark, contained higher level of total bioactive contents compared to Prikro extracts, detected by high‐performance liquid chromatography with photodiode‐array detection (HPLC‐DAD). Only the methanolic extracts irrespective of plant parts/location, showed inhibition against acetylcholinesterase (1.42–2.12 mg galantamine equivalent (GALAE)/g), while only the stem bark methanolic extract of A. djalonensis from Mafiblé was found to inhibit butyrylcholinesterase (0.65 mg GALAE/g). Thus, findings from this study could be useful for better application of the medicinal benefits from this plant.

Uncovering chemical profiles, biological potentials, and protection effect against ECM destruction in H2O2‐treated HDF cells of the extracts of Stachys tundjeliensis

“…[ 107 ] Docking grid files were generated using the cocrystal ligand binding coordinates with the aid of MGLTools 1.5.6 software, which merged all hydrogen atoms and added gasteiger partial charges to all atoms. Docking was performed using AutoDock 4.2.6 (https://autodock.scripts.edu), [ 108 ] with a docking protocol described in Sinan et al [ 109 ] Docking binding energy scores in kcal/mol were computed and Biovia DS Visualizer v4.5 (BIOVIA) was used to examine protein‐ligand interaction.…”

Exploring Chemical Composition, Antioxidant, Enzyme Inhibitory and Cytotoxic Properties of Glaucium acutidentatum Hausskn. & Bornm. from Turkey Flora: A Novel Source of Bioactive Agents to Design Functional Applications

“…As a result of this procedure, every hydrogen atom was united and given a Gasteiger partial charge. To dock, AutoDock 4.2.6 (https://autodock.scripts.edu/ accessed on 2 February 2024) was used while implementing a previously described docking method [42]. Using Biovia DS Visualizer v4.5 (BIOVIA, San Diego, CA, USA), protein-ligand interaction was investigated, and docking binding energy scores in kcal/mol were computed.…”

“…Glycosides of quercetin, isorhamnetin, and kaempferol were characterized by both fragmentation ions at m/z 301, 314, and 285, respectively, derived from aglycone core, after neutral loss of sugar (−162 Da, −132 Da, −146 Da) moiety and diagnostic RDA fragments [46]. Among them six compounds identified as isorhamnetin 3-O-rutinoside (37), isoquercitrin (38), kaempferol-7-O-hexoside (39), kaempferol-3-O-hexoside-pentoside (40), quercetin-3-O-rhamnoside isomer (41), isorhamnetin-3-O-hexoside (42) were found exclusively in methanolic extracts. Rutoside (36) was the only one identified in all five samples.…”