1989
DOI: 10.1007/bf02661805
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Chemical characterization and physicochemical behavior of biosurfactants

Abstract: Microbes have been isolated from soil and water samples after an enrichment culture process with kerosene and tested for biosurfactant production by measuring the surface and interfacial tensions and emulsification power of culture broths. The isolation and characterization of extracellular surface‐active agents from the culture broth of Pseudomonas aeruginosa 44T1 strain have been made. Preliminary structure identification with specific TLC reagents of the CHCl3MeOH (2:1) extracts showed two spots with a glyc… Show more

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Cited by 109 publications
(46 citation statements)
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“…Pseudomonas species is well known for its capability to produce rhamnolipid biosurfactant with potential surface active properties when grown on different carbon substrates (Parra et al, 1989;Koch et al, 1988;Mercade et al, 1993). Reports have been published on production of rhamnolipid biosurfactant from pure substrate (Santos er al., 1984;Mercade et ul., 1988;Santos et al, 1986), but kinetic studies on biosurfactant production from wastes are rarely published.…”
Section: Introductionmentioning
confidence: 99%
“…Pseudomonas species is well known for its capability to produce rhamnolipid biosurfactant with potential surface active properties when grown on different carbon substrates (Parra et al, 1989;Koch et al, 1988;Mercade et al, 1993). Reports have been published on production of rhamnolipid biosurfactant from pure substrate (Santos er al., 1984;Mercade et ul., 1988;Santos et al, 1986), but kinetic studies on biosurfactant production from wastes are rarely published.…”
Section: Introductionmentioning
confidence: 99%
“…7,10-DHOD prepared in the Pseudomonas sp. 42A2 system exhibited a surface tension of 30 mN/m at 50 7C in distilled water [8] and, like 7,10-DHOD prepared in our laboratory [5], did not display the unusual surface tension as shown by 7,10,12-THOD. A detailed investigation of the surface tension properties of hydroxy fatty acids is in progress and will be reported later.…”
Section: Thementioning
confidence: 98%
“…We therefore began to investigate a reactor process designed for 7,10-DHOD [5] to produce gram quantities of 7,10,12-THOD by P. aeruginosa strain NRRL B-23260. In the production of 7,10-DHOD, a new aeration mechanism was used to deal with the coexistence of a hydrophobic substrate (OA) and the surface-active property of 7,10-DHOD [7,8], which were prone to foam formation. Namely, the typical aeration through a bottom sparger in a reactor vessel was replaced by a new arrangement that combined a constant airflow from two ports on the headplate and, when needed, through a bottom sparger, in conjunction with the use of marine impellers for agitation.…”
Section: Production Of 71012-thod In a Bioreactormentioning
confidence: 99%
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“…Different bacteria produce different classes of biosurfactants which serve varied purposes in the producer strains . Rhamnolipids are glycolipids biosurfactants produced by Pseudomonas aeruginosa, which emulsify oil and reduce the surface tension of water from 72 mN m −1 to around 25-30 mN m −1 (Itoh et al, 1971;Parra et al, 1989). They find applications in tertiary petroleum recovery , decontamination of marine oil pollution, soil remediation (Banat, 1995;Lotfabad et al, 2009) and crop protection (Stanghellini and Miller, 1997).…”
Section: Future Prospectsmentioning
confidence: 99%