Chemical Characterization and Wound Healing Property of Jacaranda decurrens Cham. (Bignoniaceae): An Experimental Study Based on Molecular Mechanisms

Abstract

Background. Jacaranda decurrens Cham., known as carobinha, is prevalent in the Cerrado biome and presents popular use in treatment of dermatological diseases. The present study aimed to investigate the healing action of topical formulation of Jacaranda decurrens Cham. (FtEHJ) in mice cutaneous lesions. Methods. Phytochemical analysis of J. decurrens hydroalcoholic extract was carried out by using HPLC-PDA-ESI-MS and FIA-ESI-IT-MSn. Swiss mice were treated topically with formulation base (FtB) or Fibrinase® or ointment FtEHJ (15 mg/g; 50 mg/Kg). At the end of treatment periods, the inflammatory cytokines (TNF-α, IL-1β, and IL-6) in the lesions were measured by using ELISA and gene expression of TGF-β, Collagen I, and Collagen III was demonstrated by RTqPCR method and histological evaluation. Results. Ten compounds were identified in the extract, distributed among the classes of flavonoids and triterpenes. Treatment with FtEHJ increased the wound contraction in 24 hours, such as reduction of TNF-α, IL-1β, and IL-6 (pg/mL) cytokines in the lesion. The TGF-β and collagen gene expression was increased and the wound closure accelerated to nine days, with discrete inflammation, collagenization, and accented reepithelialization. Conclusions. The results obtained suggest chemical compounds present in the FtEHJ accelerates wound healing by being a gene expression modulator, and protein content of different molecules are involved in tissue repair.

The wound healing effect of botanicals and pure natural substances used in in vivo models

“…Regarding Jacaranda decurrens Cham., metabolic profiling was done to find out ten compounds in the extract of flavonoidal and triterpenoidal nature. It was concluded that these compounds improved the healing of wounds in this study (Serra et al 2020). Hydroethanolic extract of leaves of Lafoensia pacari A. St.-Hil.…”

Antimicrobial Activity of Extracts from the Humiria balsamifera (Aubl)

“…The samples were then filtered using a 0.22-µm PTFE filter and dried. The extract was diluted to 10 µg/mL in the HPLC solvent, and then, aliquots of 20 µL were injected directly into the LC-ESI-IT/MS [ 56 ].…”

“…The sample was injected into the HPLC system, where it was analyzed online by ESI-MS in the negative ion mode with a UV detector. Mass spectrometry was performed in an LCQ Fleet Ion Trap LC/MSⁿ—Thermo Scientific ® (Waltham, MA, EUA) [ 56 ].…”

“…The product ions were subjected to further fragmentation under the same conditions, until no more fragments were observed. The identification of different compounds in the chromatographic profile of the hydroalcoholic extract was carried out by comparing their retention times and UV spectra with the literature data [ 56 ].…”

Análise das atividades farmacológicas da Handroanthus serratifolius (Vahl) S.Grose

“…Espécies de Tecoma, em estudos in vitro, evidenciaram atividade contra o Zika vírus (Reis et al, Research, Society and Development, v. 11, n. 2, e5611222891, 2022 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v11i2.22891 3 2020). A Jacaranda decurrens Cham exibiu atividade cicatrizante de feridas cutâneas (Serra et al, 2020). Enquanto a Cecropia pachystachya apresentou atividade antibacteriana a partir de extratos aquosos e etanólicos contra cepas de Staphylococcus aureus (Costa & Hoscheid, 2018).…”