Chemical Characterization, Crossfeeding and Uptake Studies on Hydroxamate Siderophore of Alcaligenes faecalis

Sayyed, R. Z.; Chincholkar, S. B.; Meyer, Jean Marie; Kale, S. P.

doi:10.1007/s12088-011-0129-y

Cited by 9 publications

(4 citation statements)

References 19 publications

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“…Hydroxamic acids, for example, are widely found as functional groups of bacterial antibiotics ( 64 ) and siderophores ( 65 ). A. faecalis has also been reported to produce hydroxamate-containing siderophores ( 66 ). However, the biosynthesis of siderophores seems unlikely in our experiments, considering that A. faecalis was cultured with an excess of iron.…”

Section: Discussionmentioning

confidence: 99%

Hydroxylamine production by Alcaligenes faecalis challenges the paradigm of heterotrophic nitrification

Lenferink,

Bakken,

Jetten

et al. 2024

Sci. Adv.

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Heterotrophic nitrifiers continue to be a hiatus in our understanding of the nitrogen cycle. Despite their discovery over 50 years ago, the physiology and environmental role of this enigmatic group remain elusive. The current theory is that heterotrophic nitrifiers are capable of converting ammonia to hydroxylamine, nitrite, nitric oxide, nitrous oxide, and dinitrogen gas via the subsequent actions of nitrification and denitrification. In addition, it was recently suggested that dinitrogen gas may be formed directly from ammonium. Here, we combine complementary high-resolution gas profiles, 15 N isotope labeling studies, and transcriptomics data to show that hydroxylamine is the major product of nitrification in Alcaligenes faecalis . We demonstrated that denitrification and direct ammonium oxidation to dinitrogen gas did not occur under the conditions tested. Our results indicate that A. faecalis is capable of hydroxylamine production from an organic intermediate. These results fundamentally change our understanding of heterotrophic nitrification and have important implications for its biotechnological application.

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Section: Discussionmentioning

confidence: 99%

Hydroxylamine production by Alcaligenes faecalis challenges the paradigm of heterotrophic nitrification

Lenferink,

Bakken,

Jetten

et al. 2024

Sci. Adv.

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“…Production of catecholate and hydroxamate types of siderophore has been reported by Sayyed and Chincholkar (2006) . Sayyed and Chincholkar (2011) and Valdez-Nuñez and Rivera-Jacinto (2022) reported the bioremediation of heavy metal-contaminated soil using siderophore-producing microorganisms. Akhtar et al (2021) reported the potential of B. cereus to improve plant growth under chromium-contaminated soil.…”

Section: Discussionmentioning

confidence: 99%

“…Siderophores are small molecular weight ferric-specific ligands produced by various microorganisms for their iron nutrition ( Sayyed and Chincholkar, 2011 ). Although siderophores are ferric-specific ligands, they can also bind multiple other metal ions in the environment ( Schwyn and Neiland, 1987 ; Payne, 1994 ).…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of bioemulsifier by Parapedobacter indicus

Devale

Sawant²,

Pardesi³

et al. 2023

Front. Microbiol.

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The current study evaluated Parapedobacter indicus MCC 2546 for its potential to produce a bioemulsifier (BE). Screening methods performed for BE production by P. indicus MCC 2546 showed good lipase activity, positive drop collapse test, and oil-spreading activity. Furthermore, it showed maximum emulsification activity (225 EU/ml) and emulsification index (E24 50%) at 37°C in Luria Bertani broth at 72 h with olive oil as a substrate. The optimal pH and NaCl concentration for maximum emulsification activity were 7 and 1%, respectively. P. indicus MCC 2546 lowered the surface tension of the culture medium from 59.65 to 50.42 ± 0.78 mN/m. BE produced was composed of 70% protein and 30% carbohydrate, which showed the protein–polysaccharide nature of the BE. Furthermore, Fourier transform infrared spectroscopy analysis confirmed the same. P. indicus MCC 2546 showed a catecholate type of siderophore production. This is the first report on BE and siderophore production by the genus Parapedobacter.

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“…Strains lacking fhuE were more sensitive to the chelator suggesting FhuE may be important for uptake of siderophores produced by F. columnare . FhuA, FecA, and the other identified outer membrane receptors may assist with the uptake of F. columnare siderophores or they could be used to take up siderophores produced by other bacteria as is seen in other pathogens ( Magarinos et al., 1994 ; Sayyed et al., 2011 ). FhuA, FecA, and the 12 other predicted TonB-dependent iron receptors ( Table 1 ) may take up iron complexed with other (non-siderophore) molecules.…”

Section: Discussionmentioning

confidence: 99%

Flavobacterium columnare ferric iron uptake systems are required for virulence

Conrad

Evenhuis

Lipscomb

et al. 2022

Front. Cell. Infect. Microbiol.

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Flavobacterium columnare, which causes columnaris disease, is one of the costliest pathogens in the freshwater fish-farming industry. The virulence mechanisms of F. columnare are not well understood and current methods to control columnaris outbreaks are inadequate. Iron is an essential nutrient needed for metabolic processes and is often required for bacterial virulence. F. columnare produces siderophores that bind ferric iron for transport into the cell. The genes needed for siderophore production have been identified, but other components involved in F. columnare iron uptake have not been studied in detail. We identified the genes encoding the predicted secreted heme-binding protein HmuY, the outer membrane iron receptors FhuA, FhuE, and FecA, and components of an ATP binding cassette (ABC) transporter predicted to transport ferric iron across the cytoplasmic membrane. Deletion mutants were constructed and examined for growth defects under iron-limited conditions and for virulence against zebrafish and rainbow trout. Mutants with deletions in genes encoding outer membrane receptors, and ABC transporter components exhibited growth defects under iron-limited conditions. Mutants lacking multiple outer membrane receptors, the ABC transporter, or HmuY retained virulence against zebrafish and rainbow trout mirroring that exhibited by the wild type. Some mutants predicted to be deficient in multiple steps of iron uptake exhibited decreased virulence. Survivors of exposure to such mutants were partially protected against later infection by wild-type F. columnare.

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Chemical Characterization, Crossfeeding and Uptake Studies on Hydroxamate Siderophore of Alcaligenes faecalis

Cited by 9 publications

References 19 publications

Hydroxylamine production by Alcaligenes faecalis challenges the paradigm of heterotrophic nitrification

Hydroxylamine production by Alcaligenes faecalis challenges the paradigm of heterotrophic nitrification

Production and characterization of bioemulsifier by Parapedobacter indicus

Flavobacterium columnare ferric iron uptake systems are required for virulence

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