Chemical characterization of an aqueous extract and the essential oil of Tithonia diversifolia and their biocontrol activity against seed-borne pathogens of rice

Dongmo, Albert Nanfack; Julienne, Nguefack; Dongmo, J.B. Lekagne; Fouelefack, Francois Romain; Azah, Rene Udom; Nkengfack, Ephrem Augustin; Stefani, Emilio

doi:10.1007/s41348-021-00439-w

Cited by 10 publications

(4 citation statements)

References 33 publications

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“…The efficacy of the STLs-rich extracts against AAT-causing animal trypanosomiasis complements several other pharmacological properties of T. diversifolia towards discovering potent phyto-molecules. [7][8][9][10][11][12][13][14][15][16][17][18][19]21,23,28 This study identified the abundance of STLs in the extract of T. diversifolia and examined their potential as a promising source of anti-trypanosomal leads.…”

Section: Resultsmentioning

confidence: 99%

“…Gray (Asteraceae) has maintained its vital roles in phytomedicine due to its medicinal, agricultural, and ethnopharmacological relevance. [7][8][9][10][11][12][13][14][15][16][17][18][19] In folk medicine, it is claimed that the weed serves as forage for livestock and medicine for nagana in North-Central and South Western Nigeria. 20 This claim has been scientifically validated in different reports.…”

Section: Introductionmentioning

confidence: 99%

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Sesquiterpene Lactone-Rich Extract of Tithonia diversifolia (Hemsley) A. Gray (Asteraceae) suppresses Trypanosoma brucei brucei in both In Vivo and In Vitro Experimental Models

2022

TJNPR

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Tithonia diversifolia has continued to play vital roles in phytomedicine due to its ethnopharmacological relevance, medicinal properties and agricultural applications. Its richness in sesquiterpene lactones has also continued to generate interest due to its diverse pharmacological activities, structure-activity relationships, and pharmacokinetics. The study evaluated the anti-trypanosomal and cytotoxic activities of the sesquiterpene lactone (STL) constituents of Tithonia diversifolia using in vivo mouse and in vitro experimental models. The T. diversifolia methanol extract was fractionated by solvent-solvent partitioning in n-hexane, EtOAc, and n-butanol and further separated by the vacuum liquid chromatographic (VLC) method. The in vitro anti-trypanosomal and cytotoxic activities were evaluated using resazurinbased cell viability assays. The in vivo anti-trypanosomal activity was determined using a hematocrit-based packed cell volume analysis and rapid matching counting of Trypanosoma brucei brucei inhibition methods in a mouse model. The two STL-enriched sub-fractions (VLC-3 and VLC-4) obtained from the VLC separation displayed significant (p < 0.05) in vitro activity against Trypanosoma brucei with IC50 values of 0.88 and 0.52 µg/mL respectively and SI of 44 and 165 respectively. In the in vivo mouse model, a 400 mg/kg dose of VLC-3 and VLC-4 elicited a 100% clearance of the parasites within 16 and 14 days post-induction of parasitemia compared with a similar effect of the positive control drug, diminazene aceturate on day 12. The strong in vivo/in vitro anti-trypanosomal activity of T. diversifolia STL represents a promising starting point for discovering potent trypanocidal agents against T.b. brucei, the major cause of animal African trypanosomiasis.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Sesquiterpene Lactone-Rich Extract of Tithonia diversifolia (Hemsley) A. Gray (Asteraceae) suppresses Trypanosoma brucei brucei in both In Vivo and In Vitro Experimental Models

2022

TJNPR

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“…This biopesticidal activity of the essential oil was also detected in bacteria (Xanthomonas oryzae pv. oryzae and Pseudomonas fuscovaginae) at a concentration of 125 µg/mL and fungi (Bipolaris oryzae and Fusarium moniliforme) at 5000 µg/mL, which are agricultural pests that affect rice seeds in storage, demonstrating that the essential oil of T. diversifolia can be used as an alternative to synthetic fumigants (Dongmo et al 2021). Arantes et al 2024 also showed the pesticidal activity of the main components of T. diversifolia essential oil (α-pinene and β-pinene) in controlling the cattle tick Rhipicephalus (Boophilus) microplus.…”

Section: Discussionmentioning

confidence: 99%

Chemical Composition and Acaricidal and Insecticidal Activity of the Essential Oil of Tithonia Diversifolia (Asteracea) (Hemsl.) a. Gray

Junior,

Fragoso,

Paraguassu

et al. 2024

Preprint

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Tithonia diversifoliais a plant from the Asteraceae family that, due to the large quantity of hydrogenated monoterpenes present in its essential oil, can be used to prepare a product capable of combating agricultural pests. Therefore, the aim of this study was to carry out a phytochemical screening of the components of the essential oil produced by hydrodistillation of the leaves of Tithonia diversifoliaand to verify its possible acaricidal applications in the control of the spider mite Tetranychus urticae (Koch) (Acari: Tetranychidae) and insecticidal applications in the control of the corn weevil Sitophilus zeamais (Motsch) (Coleoptera: Curculionidae), both via fumigation. Gas chromatography coupled with mass spectrometry revealed the presence of 32.67% β-pinene (6,6-dimethyl-2-methylenebicycle, heptane), 24.74% α-pinene (2,6,6-trimethylbicycle, hept-2-ene) and 22.69% limonene (1-methyl-4-(1-methyleneyl)-cyclohexene), among other components, in the essential oil of T. diversifolia. Fumigation tests revealed 52% mortality of spider mites at a concentration of 20 µL/L air and 96% at 80 µL/L air. The insecticidal activity tests on the corn weevil showed no mortality, but the repellency tests on this same insect showed promising results close to 100% at a concentration of 10 µL/L air, suggesting further studies to implement its use in greenhouses to control spider mites and in silos to repel corn weevil due to its volatility.

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“…Lantana camara has been reported to contain more complex polymers and thus possibly requires more microbial categories to break down into agriculturally useful material. For example, the phytochemical composition analysis of L. camara showed 23.3% crude fiber, 26% cellulose, 16.2% lignin, and 21% hemicellulose, while T. diversifolia contains 11.2%, 17%, 7%, and 16% of these elements respectively [15][16][17]. Furthermore, the different complexities of the composting material could require assorted composting times to produce a biogeochemically stable product.…”

Section: Introductionmentioning

confidence: 99%

Evolution of fungal and non-fungal eukaryotic communities in response to thermophilic co-composting of various nitrogen-rich green feedstocks

Matheri,

Kambura,

Mwangi

et al. 2023

PLoS ONE

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Thermophilic composting is a promising soil and waste management approach involving diverse micro and macro-organisms, including eukaryotes. Due to sub-optimal amounts of nutrients in manure, supplemental feedstock materials such as Lantana camara, and Tithonia diversifolia twigs are used in composting. These materials have, however, been reported to have antimicrobial activity in in-vitro experiments. Furthermore, the phytochemical analysis has shown differences in their complexities, thus possibly requiring various periods to break down. Therefore, it is necessary to understand these materials’ influence on the biological and physical-chemical stability of compost. Most compost microbiome studies have been bacterial-centric, leaving out eukaryotes despite their critical role in the environment. Here, the influence of different green feedstock on the fungal and non-fungal eukaryotic community structure in a thermophilic compost environment was examined. Total community fungal and non-fungal eukaryotic DNA was recovered from triplicate compost samples of four experimental regimes. Sequencing for fungal ITS and non-fungal eukaryotes; 18S rDNA was done under the Illumina Miseq platform, and bioinformatics analysis was done using Divisive Amplicon Denoising Algorithm version 2 workflow in R version 4.1. Samples of mixed compost and composting day 84 recorded significantly (P<0.05) higher overall fungal populations, while Lantana-based compost and composting day 84 revealed the highest fungal community diversity. Non-fungal eukaryotic richness was significantly (P< 0.05) more abundant in Tithonia-based compost and composting day 21. The most diverse non-fungal eukaryotic biome was in the Tithonia-based compost and composting day 84. Sordariomycetes and Holozoa were the most contributors to the fungal and non-fungal community interactions in the compost environment, respectively. The findings of this study unravel the inherent influence of diverse composting materials and days on the eukaryotic community structure and compost’s biological and chemical stability.

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Chemical characterization of an aqueous extract and the essential oil of Tithonia diversifolia and their biocontrol activity against seed-borne pathogens of rice

Abstract: Che mical characte rization of an aqueous extract and the essential oil of Tithonia diversifolia and their biocontrol activity against seed-borne pathogens of rice

Cited by 10 publications

References 33 publications

Sesquiterpene Lactone-Rich Extract of Tithonia diversifolia (Hemsley) A. Gray (Asteraceae) suppresses Trypanosoma brucei brucei in both In Vivo and In Vitro Experimental Models

Sesquiterpene Lactone-Rich Extract of Tithonia diversifolia (Hemsley) A. Gray (Asteraceae) suppresses Trypanosoma brucei brucei in both In Vivo and In Vitro Experimental Models

Chemical Composition and Acaricidal and Insecticidal Activity of the Essential Oil of Tithonia Diversifolia (Asteracea) (Hemsl.) a. Gray

Evolution of fungal and non-fungal eukaryotic communities in response to thermophilic co-composting of various nitrogen-rich green feedstocks

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