Chemical characterization of<i>Cassia fistula</i>polysaccharide (CFP) and its potential application as a prebiotic in synbiotic preparation

Dawood, Dawood H.; Darwish, Mohamed Samir; El-Awady, Asmaa A.; Mohamed, Azza H.; Zaki, Ahmed A.; Taher, Mohamed A.

doi:10.1039/d1ra00380a

Cited by 16 publications

(14 citation statements)

References 71 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both strains were obtained from the bacterial strain collection in Food Microbiology Laboratory (Dairy department, Faculty of Agriculture, Mansoura University, Egypt). An overnight culture of both strains was activated in nutrient broth (NB) (BD Difco™, Bacton, Dickinson and Company Sparks, MD, USA) at 37 °C for 16–18 h. The culture was diluted on test days in buffered peptone water to a final concentration of 1.5 × 10 8 colony forming units (CFU)/mL cell counts were assessed by pour plate method, one mL of a suitable dilution was incubated on nutrient agar at 37 °C for 24 h. colonies were then counted and converted to CFU/mL ( Dawood et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…Prebiotic index (PI) was estimated as previously described ( Dawood et al, 2021 ). E. coli Nissle 1917 was used as the representative probiotic strain.…”

Section: Methodsmentioning

confidence: 99%

“…Prebiotic activity scores (P score ) were calculated as previously described ( Dawood et al, 2021 ):

where P score is prebiotic activity score; Log P is log of CFU/mL after 24 h (P 24 ) and zero time (P 0 ) of incubation at 37 °C. Log E is log of CFU/mL of K 12 at zero time (E 0 ) and 24 h (E 24 ) of culture on glucose and pure compounds.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Isolation and biological activities of compounds from Rumex vesicarius L. and their use as a component of a synbiotic preparation

et al. 2022

Self Cite

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

“…Prebiotic index (PI) was estimated as previously described ( Dawood et al, 2021 ). E. coli Nissle 1917 was used as the representative probiotic strain.…”

Section: Methodsmentioning

confidence: 99%

“…Prebiotic activity scores (P score ) were calculated as previously described ( Dawood et al, 2021 ):

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Isolation and biological activities of compounds from Rumex vesicarius L. and their use as a component of a synbiotic preparation

et al. 2022

Self Cite

View full text Add to dashboard Cite

“…MRS broths without glucose or with 2% of glucose and inulin were used as the negative control, positive control, and prebiotic standard, respectively. A preb was calculated as previously reported by Dawood, et al [ 28 ].

…”

Section: Methodsmentioning

confidence: 99%

In Vitro Antidiabetic, Antioxidant, and Prebiotic Activities of the Chemical Compounds Isolated from Guizotia abyssinica

et al. 2022

Self Cite

View full text Add to dashboard Cite

India and Ethiopia employ Guizotia abyssinica (niger plant) as a source of edible vegetable oil. Previous studies have documented the niger plant’s antioxidant properties and dietary benefits. Here, G. abyssinica extract was obtained and ten known bioactive components (1–10) were isolated. The antioxidant, antidiabetic, and prebiotic properties of whole extract and isolated components of niger and the plant’s ability to cooperate symbiotically with probiotic strains were examined. Compound 10, myricetin-3-O-L-rhamnoside, had the highest antioxidant capacity measured in the 2,2-diphenylpicrylhydrazyl (DPPH, 4629.76 ± 6.02 µmol Trolox equivalent/g compound) and ferric-reducing antioxidant power (FRAP, 2667.62 ± 7.5 mol Trolox equivalent/g compound) assays. The lowest α-amylase and glycogen phosphorylase activities and glucose diffusion were obtained with whole G. abyssinica extracts, whereas compounds 8–10 had moderate inhibitory effects. G. abyssinica extract also induced the highest glucose absorption by yeast cells in the presence of 5 mM of glucose. Moreover, Lactobacillus plantarum and L. rhamnosus incubated with β-sitosterol 3-O-D-glucoside (compound 7) showed the highest prebiotic activity score. The levels of L-(+)-lactic acid isomer in the probiotic strains were the highest in presence of the whole extract and decreased progressively in the presence of flavonoid glycosides (compounds 8–10) and β-sitosterol 3-O-D-glucoside. The enzymatic profile of the probiotic strains was unaffected by the niger extract and compounds 7–10. The findings revealed that the biological activities of G. abyssinica extract are mediated by the compounds 1–10, and it may be considered as a promising plant for the treatment of diabetes mellitus.

show abstract

“…This mode of action confers probiotics to significantly exert the composition of the colonic microbiota in the gastrointestinal tract, thus in turn enhancing host health (Lee et al, 2002 ; Vergara et al, 2010 ). A few articles (Yoo et al, 2012 ; Ajanth Praveen et al, 2019 ; Seong et al, 2019 ; Andreani et al, 2021 ; Dawood et al, 2021 ; Zhou et al, 2022 ) confirm that Lactobacillus also has some prebiotic properties. Therefore, how to maximize the value of the symbiotic relationship between prebiotics and probiotics is the focus of current research.…”

Section: Introductionmentioning

confidence: 99%

The prebiotic properties of polysaccharides obtained by differentiated deproteinization methods from Flos Sophorae Immaturus on Lactobacillus fermentum

et al. 2022

View full text Add to dashboard Cite

The polysaccharides derived from various deproteinization methods were prepared from Flos Sophorae Immaturus (FSI) to investigate the prebiotic efficacy of Lactobacillus fermentum (L.f ). The implications of polysaccharides from FSI (PFSI) gained after purification performed by non-deproteinization and different deproteinization processes (Savage method, papain method, and TCA method) via one-factor optimization were firstly investigated for the influences on the growth of L.f. The utilization of carbohydrate sources and the synthesis of protein and lactate during its growth were analyzed, as well as the variations of LDH, SOD, and GSH- Px enzyme dynamics. The results showed that the one-factor optimization of the deproteinization process with the protein removal rate and polysaccharide retention rate as the indexes led to the optimal methods of the Sevage method with 5 elution times, papain method with 80 U/mL concentration, and TCA method with 2.5 ratio, respectively. In addition, the PFSI obtained with or without deproteinization purification had a certain effect on promoting L.f proliferation. Moreover, the PFSI gained by the third deproteinization purification, at a concentration of 10 g/L, significantly elevated L.f biomass and growth rate compared with the blank control, and the utilization of reducing sugars and the synthesis of protein and lactic acid were higher than the control (P < 0.05); improved LDH, SOD, and GSH-Px activity in L.f (P < 0.05), and the TCA method could be effectively applied to eliminate the proteins affecting FSI in probiotics, and PFSI may be a potentially beneficial prebiotic and intestinal reinforcer.

show abstract

Chemical characterization ofCassia fistulapolysaccharide (CFP) and its potential application as a prebiotic in synbiotic preparation

Abstract: CFP may be a prebiotic due to its high resistance against α-amylase and acid. CFP provides two ways to adjust nitric oxide synthesis in macrophages. We show the use of CFP in the production of synbiotic yogurt with favorable physical and chemical characteristics.

Cited by 16 publications

References 71 publications

Isolation and biological activities of compounds from Rumex vesicarius L. and their use as a component of a synbiotic preparation

Isolation and biological activities of compounds from Rumex vesicarius L. and their use as a component of a synbiotic preparation

In Vitro Antidiabetic, Antioxidant, and Prebiotic Activities of the Chemical Compounds Isolated from Guizotia abyssinica

The prebiotic properties of polysaccharides obtained by differentiated deproteinization methods from Flos Sophorae Immaturus on Lactobacillus fermentum

Contact Info

Product

Resources

About